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The blastula formation whose embryos were from different sources was compared and the blastomere number, quality classification and the relationship between the parity of the blastomere number and the blastula formation at d 3 were studied.

比较不同来源胚胎囊胚形成情况和d3胚胎的卵裂球数、质量分级等;并利用废弃胚胎囊胚形成情况对体外受精妊娠结局进行预测。

Because current federal law already forbids the use of federal funds to create embryos (the earliest stage of human offspring ago birth) for research or to 清楚ingly endanger an embryo's life, NBAC will remain silent on embryo research.

因该现今的联邦国家律法已经禁止应用联邦基金克隆胚胎(人类后裔在出生前的最早阶段)用于研究或者有意地威胁胚胎的生命,NBAC在胚胎研究上将保持沉默。

Because current federal law aleasysty tobids the use of federal funds to create embryos (the earliest stage of people offsummer ago birth) to research or to 清楚ingly endanger an embryo's life, NBAC be to remain silent on embryo research.

因该现今的联邦国家律法已经禁止实际运用联邦基金克隆胚胎(人类后裔在出生前的最早阶段)用于研究或者有意地威胁胚胎的生命,NBAC在胚胎研究上将保持沉默。

Results:LIF improved the development rate of early embryo at the concentration of 0.1,1 and 10ng/ml(P.05).The enhanced effects of LIF were significant for morula and blastocyst stage,not for 1~8 cell stage of embryos.Conclusion:LIF may improve the development of early mouse embryos at the appropriate concentration.

结果:①0.1ng/ml、1ng/ml和10ng/ml浓度的LIF对小鼠早期胚胎的发育和胚泡的形成有促进作用,0.01ng/ml和100ng/ml浓度的LIF对胚胎的发育无明显影响;②LIF对小鼠早期胚胎发育的影响主要发生于桑椹胚和胚泡期,对1~8细胞期胚胎无明显影响。

Results coculture with endometrial epithelial cells could promote the formation of mouse morula (71.3% and 61.2%,p.05) and blastocyst (50.0% and 39.7%,p.05), and increase the blastocyst hatched rate (34.8% and 24.3%,p.05) and total cell number of blastocyst (44.1 and 35.6, p.05).conclusion coculture with endometrial epithelial cells can facilitate the development of early mouse embryo in vitro, which can optimize the condition of embryonic development in vitro.

结果:与无上皮细胞相比,共培养明显促进了小鼠桑椹胚率(71.3%和61.2%,p.05),囊胚率(50.0%和39.7%,p.05),囊胚孵化率(34.8%和24.3%,p.05)和胚胎总细胞数(44.1和35.6,p.05)。结论:子宫内膜上皮细胞能够促进小鼠胚胎的体外发育,对早期胚胎的体外发育环境具有优化作用。

With Mab 2A3, the first positive cells were detected at the limb bud stage of the embryos. Distinguishable positive haemocytes with a diameter of about 7μm appeared in embryonic-nauplius. At the following nauplius I stage, configuration of haemocytes became more integrated and clear. Their diameters were almost similar to those of haemocytes from adult shrimp. With Mab 1H11, the first positive granular haemocytes were identified at the stage of embryonic-nauplius which existed in clusters with positive punctate fluorescence of granules distributed around the periphery of granular haemocytes.

通过对包括囊胚期、原肠胚期、肢芽期、膜内无节幼体期和无节幼体Ⅰ期在内的中国对虾胚胎的间接免疫荧光检测,最早在肢芽期胚胎中检测到2A3阳性信号;在膜内无节幼体期,2A3阳性血细胞平均直径约7μm,聚集成簇;在无节幼体Ⅰ期,血细胞形态更加完整清晰,平均直径已与成体对虾血细胞相似。1H11阳性大颗粒细胞最早在膜内无节幼体期胚胎中出现,这些细胞同样成簇存在,在其细胞质外围可见点状荧光。

BACKGROUND: Compared with adult mesenchymal stem cells, yolk sac-derived MSCs of early embryo have stronger self-renewal capacity and plasticity. Moreover, they could differentiate into angioblast and hemopoietic stem cells during embryonic development and trigger embryonic angiogenesis and hemopoietic tissue development.

背景:与成体间充质干细胞相比,来源于早期胚胎的卵黄囊间充质干细胞有更强的自我更新能力和可塑性,且在胚胎发育过程中可分化为成血管细胞和造血干细胞,启动胚胎血管发生和造血组织发育。

Analysis of mitochondrial DNA composition of the panda-rabit cloned embryos found that mitochondria from both panda somatic cells and rabbit ooplasm co-existed in early blastocyst, but mitochondria from rabbit ooplasm decreased and those from panda donor cells dominated in early fetuses after implantation.

结果显示,应用卵裂球电融合方法可以制作一系列的多倍体胚胎;小鼠囊胚的形成与胚胎中的细胞数目无直接关系,卵裂球的电融合和染色体数目的增加不会改变胚胎的发育进程;而胚胎细胞中的核/质比可能是控制胚胎囊胚化的一个重要因素。

The cryopreservation of fishes embryos has not reached an applicational level.The main reason is the characteristics of fishes embyros, such as big physical volume, the bad permeability of vitelline membrane, etc, which casued that traditional techniques for the incorporation of cryoprotectants could not make the cyroprotectants come into the inner part of embryos sufficiently and cryoprotectants could not protect embryos effectively.

鱼类胚胎冷冻保存经过多年研究,尽管有所突破,但目前仍未达到应用水平,其主要原因是由于鱼类胚胎体积大、卵膜通透性差等特点,导致传统的平衡法,无法使抗冻剂足够、有效的进入胚胎内部起到保护胚胎的作用。

VSD2 suitable for the eryopreservation of sea perch embryos and PM1、PM2、PM3 and PM4 suitable for flounder embryos and PMP1 suitable for turbot embryos were chosen by adjusting the ratio of cryoprotectants in BS2 and equilibrating embryos in vitrification solutions.

在基础液BS2中对抗冻剂配比进行调整,经过胚胎平衡实验,筛选出了适合于牙鲆胚胎的玻璃化液PM1、PM2、PM3和PM4,适合于大菱鲆胚胎的玻璃化液PMP1。

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