胚胎的

To observ the toxicity of infected mice and establish mice reproductive toxicity model;②To observe the infected BALB/c female mice ovulation quantums and ovum chromosome;③To observe the oestrogenic hormone( FSH, LH, E2 )level of infected BALB/c female mice;④To observe zygote developmental condition before nidation;⑤To observe embryonic development condition of infected mid-gravidity mice to research the reproductive toxicity and development toxicity of toxoplasma gondii to the BALB/c female mice.

目的： 本实验采用弓形虫感染BALB/c雌鼠，①观察染虫雌鼠的毒性反应，建立生殖毒性的小鼠模型；②染虫雌鼠排卵情况及卵母细胞染色体的变化；③染虫雌鼠性激素(FSH、LH、E2)水平的变化；④着床前受精卵的发育情况；⑤孕中期鼠染虫后胚胎的发育情况，探讨弓形虫对雌鼠生殖功能及胚胎发育的毒性作用。

Protein folding, transportation orientation. When the body is stressed, the synthesis of Hsp60 can be markedly induced to stabilize and refold proteins. Hsp60 is benefited for the function of heart as it is expressed in the different time of embryo. 3. MAPKKK4 or MEKK4 is one of the members of the major systems participating in the cellular signal transduction of MAPK.

1、在小鼠胚胎的发育中，胚胎心脏的分化和功能的完善需要大量的蛋白质参与。2、Hsp60 是一个分子量为 60kDa 的蛋白质，普遍存在于原核细胞和真核细胞中，主要存在于细胞质和线粒体基质中，其一是起分子伴侣的作用，即对新生蛋白质进行折叠、转运和定位。

But in 2007, scientists made a revolutionary breakthrough. Three different groups simultaneously announced that they had converted unipotent, mature skin cells back into an undifferentiated state. In other words, they had created cells that behaved like embryonic stem cells without using any embryo derived materials.

可是在2007年时，科学家们取得了革命性的突破，三个不同的小组同时宣布他们能够把已分化、成熟的表皮细胞转会一种未分化的状态，换句话说，他们制造了一种类似胚胎干细胞的细胞，但没用到任何源于胚胎的原料。

Kansas Senator Sam Brownback is a sponsor of a bill that outlaws the cloning of human embryos for research, because he says it's a life form that is eventually destroyed to harvest stem cells, and in his view that makes it abortion.

布朗柏科是这一个立案的提案人，立案为禁止因研究为目的的人类胚胎的复制，原因是他认为人类胚胎已经是生命了，而且那最终就是对于有收获的干细胞的摧毁，按照他的观点，那就是如同堕胎。

Methods Primary human embryonic germ cells were obtained by tissue culturing of embryonic genital ridges and mesenteries from the embryos aged 8 weeks, and then were passaged on the 8th and 16th day using pick-up and digestive passage method. Three kinds of digestive juice were used.

取8周人胚胎的生殖腺嵴和肠背系膜，进行组织块体外培养人胚胎生殖细胞，选择原代培养第8天和第16天的细胞，采用挑取传代法和消化传代法，其中的消化传代法采用三种不同的消化液进行传代培养，记录各代培养的细胞集落数。

During the first 3 days of sequence culture, SOF and granulosa cell enhanced the development of porcine parthenogenetic embryos and the cleavage rate (P.05) and the number of beyond 4-cell embryos. During the following 3 days. NCSU-23 supplemented with FCS and POEC supported the embryo to morulae/blastocysts.

在序贯培养的前3d，SOF培养基和颗粒细胞对胚胎的发育有促进作用，分裂率(P.05)和突破4细胞阻滞的数目显著增加，在培养的后3d，添加胎牛血清的NCSU-23和输卵管上皮细胞能支持较多胚胎发育到桑囊胚，桑囊胚的发育率为(59.5±3.2)%(P.05)。

In addition, we studied the distribution of mitochondria in metaphase Ⅱ rabbit oocytes, zygotes and early embryos by two active mitochondria dyes and revealed the change of active mitochondria during early development of rabbit embryos. We also found that the distribution of microfilaments and microtubules have similar patterns to that of active mitochondria, which is an important basis for further development of animal cloning study using the recipient oocytes derived from rabbit.

此外，我们应用两种线粒体荧光染料研究了日本大耳白兔体内成熟卵母细胞、正常交配后收集的受精卵及早期体外发育胚胎的活性线粒体的位置变化，首次揭示了兔早期胚胎中活性线粒体的变化特征及其与微丝微管变化的相关性，为进一步开展以兔卵母细胞作为核移植受体的动物克隆研究打下了重要的基础。

With the development of embryo, Myf5 expression decreased gradually in somites in the anterior region, but remained strong in the newly formed somites; After 30 somites formed, MyoD expression decreased in the somites except the caudal somites. At the hatching stage, MyoD and Myf5 were expressed in head muscle cells and fin muscle cells. In the growing fish, Myf5 was expressed in the skeletal muscle and intestine, and in adult flounder, Myf5 was only expressed in muscle. In the growing fish and adult fish, MyoD was only expressed in muscle.

在胚胎发育早期，Myf5在近轴中胚层中表达，体节发生过程中，Myf5在体节中表达，MyoD基因最早在分节板的体节前细胞中表达，随后在近轴细胞、体节中表达；随着胚胎的发育，Myf5在成熟体节中表达量降低，在新生体节中表达较强；MyoD自30个体节时期后只在新生的尾部体节中表达，在成熟的体节中表达量降低；在孵化期，MyoD和Myf5在头部及鳍的肌肉、尾部的体节中表达；生长期的牙鲆中，Myf5在骨骼肌和肠中表达，成体牙鲆中，Myf5只在肌肉中表达；生长期的牙鲆及成体牙鲆中，MyoD只在肌肉组织中表达。

The results show that eggs dissected from the lateral oviducts may cleave in the culture medium composed of TC - 100, plasma of larvae of its host Ostriniafurnacalis and fetal serum.

通过在体外条件下培养腰带长体茧蜂侧输卵管处解剖得到的脱去滤泡细胞的成熟卵及从寄生早期幼虫血淋巴中获得的初期胚胎，对腰带长体茧蜂卵和胚胎的初期发育进行了初步的研究。

Cryopreservation of fish gametes and embryos plays an important role in the preservation of seed resource, cryobiology, genetics breeding and aquaculture.

鱼类配子和胚胎的冷冻保存对鱼类种质资源保护、低温生物学、遗传育种和水产养殖业都具有重要的意义。胚胎的冷冻保存是一项系统工作，一个环节处理不慎，都会导致整个冷冻保存实验的失败。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。