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Microinjection experimentation proved that both expression rate of marker gene and the survival rate of transgenic embryos are high.

显微注射实验证实:在青鳉受精卵的单细胞期进行显微注射,标记基因的表达率和胚胎的存活率均较高。

In this study,we introduced the use of sodium chloride step gradients in fractionating foreign DNA fragment for microinjection.

与传统的琼脂糖凝胶回收的方法相比较,用此方法制备的外源DNA片段对小鼠受精卵进行显微注射后,受卵体母鼠的胚胎存活率,以及子代小鼠的外源基因整合率均有明显的提高。

METHODS: Spinal cord injury model produced in 42 Wistar rats on T7 by use of modified Allen's impact method (10 g x 5 cm); 3 days after injury, 20 microliters FSCS with a density of 1 x 10(5)/microliter prepared from E14 rat were injected into the epicenter of the traumatized cavity.

方法对 4 2只 Wistar成年大鼠以改良 Allen法(10 g× 5 cm)打击脊髓,3天后将孕 14天(E14 ) 5只胚胎脊髓细胞悬液 2 0 μl植入损伤空腔,移植后 2、4、6、8、10和 12周,以组织学、免疫组织化学观察移植物成活、分化及其与宿主之间关系。

Inner cell mass were separated by micromanipulator with laser micro-dissection under the microscopy.

在显微镜下利用显微操作仪及胚胎专用雷射将胚囊之内细胞质分离。

Decidual tissue is a very complex and highly concordant tiny environment between mother and embryo during pregnancy, which is constituted of the variety cells such as extravi11ous trophoblast,endometrium cells, bone marrow cells, decidua l microphage,T cells and a few B cells and so on.

正常蜕膜组织是母-胎界面间一个非常复杂而又高度协调的微环境,它由多种细胞如侵袭至蜕膜的绒毛外滋养细胞(extravi11ous trophoblast,EVT)、子宫内膜源性细胞(如上皮细胞和血管内皮细胞)、骨髓源性细胞(如大颗粒淋巴细胞、蜕膜巨噬细胞、T细胞和少许B细胞)构成,这些细胞以及由这些细胞、母体和胎儿所分泌到蜕膜的细胞因子共同形成了母-胎间信息交流的平台,并对胚胎滋养细胞"有控性"侵袭、子宫内膜的免疫容受性、蜕膜化和胎盘的形成进行精细的调控。

The influence factors to microspore embryogenesis of non-heading Chinese cabbage were studied through seventeen genotypes.

以17个不同基因型的不结球白菜为供试材料,对影响小孢子胚胎发生频率的若干因素进行了研究。

Results The microtome section of embryonic with paramesonephric duct were obtained.

结果 获取含不同发育天数中肾旁管的小鼠胚胎切片。

ResultsThe microtome section of embryonic with paramesonephric duct were obtained.

结果获取含不同发育天数中肾旁管的小鼠胚胎切片。

Objective To explore the effect of formaldehyde on differentiation and proliferation of limb bud and midbrain cells in mouse embryos.

目的 探讨甲醛对小鼠胚胎肢芽和中脑细胞分化、增殖的影响。

Objective To investigate the effects of sodium-selenite on midbrain neurons apoptosis.

目的 研究亚硒酸钠对大鼠胚胎中脑神经细胞凋亡的影响,并探讨其中可能的机制。

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He and Nina moved to California and lived at 2005 Ivar Street, Apt.

他和Nina搬到加州,并在2005年伊瓦尔街,公寓生活。

Droperidol ( potently inhibits transfected HERGchannels and this is the probable mechanism for QT prolongation.

氟哌利多有效地抑制了转染的 HERG 钾通道,可能是 QT 间期延长的机制。

The Nazi's cruel treatment of the Jews during the Second World War is beneath contempt .

二战期间纳粹对犹太人的暴行极其可鄙。