聚合酶

In which the buffer's pH was adjusted, pfu Taq DNA polymerase and Klenow fragment were combined, the organic reagent methylamine was added to uncoil the secondary structure.

本研究通过调整反应体系缓冲液pH值、采用高保真pfu DNA聚合酶和Klenow酶组合、加缓解二级结构的有机试剂甲胺、换引物多步扩增而成功实现了重复区片段R02一R11的扩增。

Intimal areas were measured using morphometric analysis of perfusion-fixed vein graft specimens, and intimal thickness was calculated using circumferential measurements. The SMC proliferation was studied by the immunohistochemical detection of proliferating cell nuclear antigen. Expression of MMP-2、MMP-9 mRNA in vein grafts and unoperated control Vein grafts was detected by reverse transcription polymerase chain reaction. Substrate gel zymography was used to determine the proteolytic activity.

分别行HE染色、Verhoeff弹性纤维染色观察组织病理变化，计算机病理图象分析系统测量新生内膜厚度及面积，免疫组织化学方法检测静脉壁增殖细胞核抗原表达以观察细胞增殖情况，半定量逆转录-聚合酶链反应检测静脉壁基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)mRNA的表达，明胶酶谱法检测MMP-2、MMP-9活性，比较各组之间的差别。

Methods Biopsy samples of were obtained from 353 patients with cervical carcinoma, HPV were detected by PCR and endonuclease cleavage. Results The results indicated that HPV types 16, 18 were found in 138 (39.1%) 353 cervical carcinoma, while HPV types 6, 11 were found in 81 (69.2%) of 117 acuminate verruca.

采用聚合酶链反应-核酸内切酶分型检测宫颈癌活检组织中HPV-DNA，对来源于子宫颈癌患者的石蜡包埋组织标本进行四种常见生殖道人乳头瘤病毒(HPV6、11、16、18)的检测和分型。

Methods Totally 200 cases of 9-12 yearold Han children without bone metabolism diseases were randomly selected. Questionnaire investigation and physical measurement of height and weight were made. BMD was measured by dual energy X ray absorptiometry. VDR gene polymorphism (cleavaged by restriction endonuclease of BsmⅠ and FokⅠ) was analyzed by PCRRFLP.

收集排除影响骨代谢疾病的西安地区9-12岁汉族儿童200例，进行问卷调查、体格测量；用双能X线骨密度仪测量腰椎BMD；用聚合酶链反应限制性片段长度多态性方法，分析VDR基因的2个限制性酶切位点BsmⅠ、FokⅠ的多态性分布。

Methods PCR-SSCP and PCR product cloning, sequencing were performed to detect mutation of p16 gene exon 2 in peripheral blood of 60 patients with arseniasis caused by coal-burning pollution,at the same time,PCR-based methylation assay was performed to analyze the methylation of p16 gene exon 1. Results No p16 gene exon 2 mutation was found in 60 cases.

采用聚合酶链反应-单链构象多态性分析和PCR产物克隆测序技术对60例砷中毒患者外周血中p16基因第2外显子突变情况进行检测，同时采用甲基化敏感的限制性内切酶方法对p16基因第1外显子甲基化情况进行分析。

The morphological and cytopathological characters of the viruses were observed by electron microscopy The types of virus were identified with I-ELISA, DAS-ELISA, TAS-ELISA, RT-PCR and IC-PCR. The CP genes of the main viruses identified in this research were amplified by PCR, cloned into pGEM-T and sequenced. The pumpkin germplasm from Heilongjiang and Yunnan provinces was screened for resistance to the main viruses using friction and roots immersed inoculation.

应用电子显微镜负染法和超薄切片法观察病原粒体形态及细胞病理学特征；利用酶联免疫吸附测定法中的双抗体夹心ELISA、间接ELISA、三抗体夹心ELISA以及反转录聚合酶链式反应、免疫捕捉PCR方法鉴定了采集样品中的病毒种类；对被确定为南瓜主要病毒病原的外壳蛋白基因进行了PCR扩增，克隆到pGEM-T载体并进行测序；应用摩擦接种法和浸根接种法对云南、黑龙江省部分南瓜品系、品种进行了抗性筛选。

Methods The system employs the 5' 3' nuclease activity of Taq DNA polymerase to digest a salmonella specific internal fluorogenic probe which is labeled with two fluorescent dyes and hybridizes to the target DNA during PCR.

利用TaqDNA聚合酶的 5' 3'核酸外切酶活性降解特异性杂交于模板上的荧光标记探针，导致荧光强度的改变，通过检测荧光强度分析PCR扩增产物。

Methods The 26th exon, the 30th exon and the 21st intron of gene GP Ⅱb in 110 individuals were amplified by polymorphism and sequenced to investigate whether there was linkage among the polymorphisms of the gene. Human platelet antigen-3 (HPA-3) gene frequency was detected by Fok 1 enzyme in 147 patients with hematologic diseases, and was compared with that in 110 normal individuals. Forty-four patients who received apheresis platelet transfusion repeatedly were randomly divided into the HPA-3 homotype group and the control group. The antibodies of the platelet were detected after 3 times of platelet transfusion.

聚合酶链反应－单链构型多态性分析检测110例正常人GP Ⅱb基因第26和30外显子(Exon 26， Exon 30)及21内含子(Intron21)基因多态性，进行基因序分析，研究这些基因多态性是否存在连锁关系；应用Fok1酶切法对147例血液病人人类血小板抗原－3(human platelet antigen-3， HPA-3)基因进行分型，并与110例正常人进行比较；将接受单采血小板输注的44例血液病人随机分为HPA-3同型输注组和对照组，血小板输注3次以后检则血小板抗体。

Methods the gene polymorphism of ACE in 108 patients were analyzed by using the polymerase chain reaction. Main renal arteries, segmental arteries and interlobar arteries in 108 patients were measured by color Doppler flow image.

用聚合酶链反应对甘肃地区108例糖尿病患者血管紧张素转换酶基因多态性进行检测，并对患者双肾肾主动脉、段动脉、叶间动脉进行彩色多普勒血流成像。

Methods 202 kinless subjects of the Chinese Han were investigated in this study, including 82 normal controls, and 120 cases diagnosed as CHD. The PPARδ+294T/C gene polymorphism was determined by polymerase chain reaction and restriction fragment length polymorphisms. The frequencies of PPARδ+294T/C genotypes and the "C"allele frequency were analyzed, to evaluate the risk for the CHD among variant genotypes.

运用聚合酶链式反应及限制酶片段长度多态性技术分析无血缘关系汉族人群[包括82例正常对照者，120 例冠心病患者]的PPARδ+294T/C基因多态性，分析基因型频率、等位基因频率，并对不同基因型患者冠心病的危险性进行评价。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。