聚合酶

Based on the fact that the dye probe can intercalate DNA duplex to emit fluorescence, a novel method for detection of DNA polymerase has been developed. The polymerization process has been indicated by fluorescent signal in real-time. Based on this assay, the influence of anti-tumour drugs on the activity of polymerase has been investigated.

利用染料探针技术，建立了一种简便快捷地实时监测聚合酶活性的方法，探讨了三种抗肿瘤药物对聚合酶活性的影响，为以聚合酶为作用靶标的抗肿瘤新药的开发及筛选提供了一种新的思路。

The results of these early research work showed that RNA polymerase Ⅰ transcription was localized in the nucleoli and RNA polymerase Ⅱ and Ⅲ in the nucleoplasm.

当时的研究结果显示：RNA聚合酶Ⅰ的转录发生在核仁内，RNA聚合酶Ⅱ和RNA聚合酶Ⅲ的位于核质中。

The results of these early research works showed that the localization of RNA polymerase I transcription was in the nucleoli and that of RNA polymerase II and Ⅲ in the nucleoplasm.

研究结果显示：RNA聚合酶Ⅰ的转录发生在核仁内，RNA聚合酶Ⅱ和RNA聚合酶Ⅲ的转录发生在核质中。

NodD binds to and bends target promoters through anchoring two tandem and individual specific DNA sites. NodD functions as a tetramer, which has a V-shaped main body. Tetrameric NodD is to change its own conformation rather than its oligomeric forms in response to small signal molecules. The specific interaction between each NodD DNA-binding domain and each specific DNA site does not alter itself in spite of naringenin induction, and the induced conformational change is transferred from protein to DNA. Only the DNA conformation incited by induced NodD is competent for RNA polymerase to form the transcriptional open complex. It cannot be excluded that NodD may have protein-to-protein contacts with RNA polymerase, and that the NodD conformational change may also directly contribute to the transcriptional open complex formation. However, the NodD conformational change itself cannot serve as the determinant of the transcriptional molecular switch.

通过研究，我们提出了初步的NodD操纵子激活模型：第一，四聚体是NodD蛋白的功能单位，它通过铆钉两个串联的相对独立的DNA靶位点结合被诱导的启动子；第二，小分子配基的结合是改变NodD四聚体的构象而不是引发不同形式的寡聚体，在我们的模型中，NodD四聚体缩小其V形主体的弯折角，进而缩短其DNA结合功能域的间距；第三，小分子信号的诱导并没有改变NodD的DNA结合域和其DNA靶位点的相互作用，NodD的构象改变由蛋白质经其双铆钉位点传递给DNA；第四，只有诱导状态的NodD激发的DNA构象才能有效地使RNA聚合酶形成转录开放复合物；第五，不排除NodD与RNA聚合酶可能有直接的相互接触位点，不排除NodD构象的改变可能直接有利于RNA聚合酶形成转录开放复合物，但是我们认为NodD构象改变本身不是充当转录激活开关的决定因素。

Probably the octamer changes position without ever completely losing contact with the DNA

当聚合酶前进时，它会在前方产生阳性的超螺旋结构，这将会取代与DNA和聚合酶保持联系，并在RNA聚合酶后嵌入八聚体。

In the past there has been a lot of emphasis on chromatin modifications and physical access to genes, but Scp1 regulates the activity of the enzyme that transcribes genes directly, he adds.

激酶可以为RNA聚合酶 II加上一个小的磷酸盐基团，使RNA聚合酶 II继续转录基因，而Scp1则会移除小的磷酸盐基团，使RNA聚合酶 II停工。

There are three types of RNA polymerases in eukaryote: polymerase I, II and Ⅲ.

真核生物细胞核中具有三种不同的RNA聚合酶，分别是RNA聚合酶Ⅰ、RNA聚合酶Ⅱ和RNA聚合酶Ⅲ。

Furthermore, by using two-colored probes to study the relationships between RNA polymerase Ⅲ and RNA polymerase I, we observed that the signals of the two RNA polymerase were very close.

同时，应用这一研究方法对RNA聚合酶Ⅰ的转录发生在核仁中的结论进行了确证，并进而应用双探针标记荧光原位杂交的方法对RNA聚合酶Ⅲ和RNA聚合酶Ⅰ转录位点的关系进行了初步探讨，结果显示两者的转录位点之间非常接近，并且有伴随发生的趋势。

It is believed by many that most viral polymerases therefore evolved from DNA polymerase and are not directly related to the multi-subunit polymerases described above.

因此很多人相信大部份的病毒聚合酶是从 DNA 聚合酶演化而来，并不是直接与上述的多亚基聚合酶有所关联。

The nuclei of eukaryotic cells contain three types of RNA polymerases: polymerase Ⅰ,Ⅱ and Ⅲ. They perform different functions in the cells.

真核生物的细胞核中具有三种不同的RNA聚合酶，分别是RNA聚合酶Ⅰ、RNA聚合酶Ⅱ和RNA聚合酶Ⅲ。

Objective: To study the effect of polycythemia on blood oxygen saturation.

裴蕾目的：观察RBC剧增而引起的高粘血症对血氧饱和度的影响。

Based on SIMPLER algorithm in the curvilinear body-fitted coordinates, the calculations were performed for Pr=0.7, Re=10~1000 on non-orthogonal non-staggered grids which are generated by elliptic equation systems.

采用曲线坐标系下压力与速度耦合的SIMPLER算法，数值研究了周期性渐扩渐缩波纹通道内脉动流动与换热情况，流动Re数的范围为10~1000，Pr数为0.7。