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Then we transfected transitorily the recombinant of green fluorescent protein gene and middle molecular weight neurofilament cDNA into wide type N2a (N2a/wt) and N2a/tau40 to observe the effect of tau accumulation on GFP-NFM fusion protein transport in cellular processes in living cells. At last we used an apoptotic inducer, camptothecin (an inhibitor of topoisomerase-1) to treat N2a/wt and N2a/tau40 cell lines, and compared their apoptotic response.

主要结果如下:一、tau蛋白过度表达和聚积对细胞形态的影响:倒置显微镜下观察两种细胞的形态,发现N2a/wt细胞的突起多而长,而N2a/tau40细胞胞体变圆,突起明显缩短;免疫印迹结果显示转染了tau40的细胞内tau的免疫反应约增加14倍,免疫荧光结果显示N2a/tau40细胞胞体内呈现出较强的红色荧光,tau主要分布在核周和突起起始部分的胞质内,而N2a/wt细胞内的荧光很弱。

Discuss the method of synthasizing the polybasic hydroxyethoxy benzene by thereaction of polybasic phenol with 2-chloroethanol in the ethanol solvent under the conditionof phase transter catalyst, which raises the product yield and shortens the reaction time.

以多元酚为原料,乙醇做溶剂,在相转移催化剂条件下与氯乙醇作用,合成多元羟乙氧基苯的方法,提高了产品产率,缩短了反应时间。

Methods The cuniform tissue of hypertrophic nipple and trigon skin of areolar were excised according to mark after local anesthesia .Pay attention to preserve the ducts.In advance we vertically suture the cut for establishment the diameter of new nipple. Once more we across suture the cut to shorten the nipple. The cut is inverted " T " after operation.

在每侧肥大乳头适当位置上设计2个相对的纵形楔型组织瓣和与之相连的乳晕小三角形皮肤瓣,局麻下切除设计线内组织,注意保护乳腺管,先纵形缝合,确立新乳头直径,再横形缝合,缩短乳头,使缝合后切口呈倒T形。

Based on Qin grounding line of tripping incidents with the relay moves conduct detailed analysis of similar incidents in the future deal with accumulated experience, Accident Analysis and shorten the processing time, and the problems which suggest improvements.

本文通过对秦王线发生的一起接地跳闸事故中继电保护的动作行为进行详细分析,为今后类似的事故处理积累经验,缩短事故分析和处理的时间,并对其中存在的问题提出改进意见。

Skewed legs are a sure sign of true vintage jeans.

缩短的裤腿是一个真实古典的牛仔裤的标志。

Truncated cloth cone used to indicate wind direction.

缩短了的锥形物用来测定风向。

Using of matrix assisted laser desorption/ionization time of flight mass spectrometry coupled with carboxypeptidase Y digestion for the analysis of C terminal peptide ladder by direct determination of the mixture of truncated peptide, the sequence of C terminus can be read out simplely from the mass differences between adjacent peptide peaks on the mass spectrum.

用羧肽酶Y水解蛋白质和多肽,结合飞行时间质谱测定酶解后缩短肽的质量,根据相邻两肽峰的质量差别读出蛋白质和多肽的C端序列,在pmol水平下测定了人促肾上腺皮质激素片段以及人血管紧张肽片段,其中人促肾上腺皮质激素片段测到了C端 15个氨基酸残基的顺序

If you lack a sense of purpose, your stay on Earth could be truncated.

如果你没有目标的意识,那你在人世上停留的日子就缩短了。

At present, domestic third party automobile logistics enterprises are still at infancy, though development, rapidly, can undertake a certain logistics function only generally, for instance storage, transportation, etc., it is difficult to shorten in products turn-round time to cause enterprises, the logistics cost can not be reduced obviously, very great with the disparity of foreign automobile logistics enterprises.

目前,国内的第三方汽车物流企业还处于刚刚起步的阶段,虽然发展迅速,但一般仅能承担某一物流功能,如仓储、运输等,导致企业产品周转时间难以缩短,物流成本降低不明显,与国外汽车物流企业的差距非常大。

It is indicated that average size of the tuyere coke .

随着喷煤量的增大,风口焦的平均粒度变小,回旋区缩短

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Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克,果实色泽鲜艳,汁多味浓,甜酸适口,离核仁甜,清末时就驰名陕、甘两省,清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异:红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感,ED90值分别为47.65、71.67和29.17g/hm2;春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感,ED90值分别为96.91、114.20g/hm2;而番茄不敏感。

However, I have an idea.

不过,我有个主意。