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The changes of population density of Rhizosphere bacteria, fungi and actinomyces, and the types and population density of nitrogen-fixing bacteria, phosphobacteria and potassium-bacteria in different types of soils were studied.

研究了不同肥力烟区根际细菌、真菌和根际放线菌的数量变化,以及固氮细菌、磷细菌和钾细菌的数量和种类的变化。

The aerobe of aerobic sewage liquid in autumn was significant more than that in winter.③In summer, autumn and winter, the anaerobe of anaerobic sewage liquid were significant more than that of aerobic sewage liquid.

3夏、秋季的厌氧沼液,厌氧培养出的细菌数极显著高于好氧沼液厌氧培养出的细菌;冬季厌氧沼液厌氧培养出的细菌数,显著高于好氧沼液厌氧培养出的细菌

Results We monitored the bacteria of the different objects in the disinfected and no-disinfected enviroment and found the additional L-form monitoring to the objects did not have the effect on the eligible rate of total number volume of antetype except of the using alcohol.L-form is easier induced when we disinfects the bibulous enviroments which have much infecants.

结果 对于医院环境消毒前后不同监测对象的细菌污染情况,除使用中的酒精外,增加监测细菌L型对细菌原型菌落总合格率没有影响;而在消毒污物"较多"和吸水性较强的环境时,则较易形成细菌L型。

Results The results showed that the average concentration of the bacteria, fungi and total microbes in the outdoor environment were 5.04×10^4, 3.75×10^3 and 5.41×10^4 cfu/m^3 respectively and the fungi accounted for 6.92% of the total. The preliminary identification showed that the dominant bacteria mainly were Micrococcus, Bacillus, Pseudomonas and Staphylococcus and the average percentage were 37.3%, 21.0%, 10.3% and 9.5% respectively. Of the five fungi the dominant ones were Aspergillum, Penicillium, Mucor and Rhizopu. The average percentage were 42.0%, 36.0%, 4.4% and 4.2% respectively. Conclusion The outdoor air of Hefei has been polluted by airborne microbes.

结果 合肥城区空气细菌总数均值为5.04×10^4cfu/立方公尺,真菌总数均值为3.75×10^3cfu/立方公尺,合计为5.41×10^4 cfu/立方公尺,其中真菌占6.92%;对不同采样点的空气细菌和真菌进行了初步鉴定,优势细菌属为微球菌属、芽孢杆菌属、假单胞菌属和葡萄球菌属,分别占总细菌的37.3%,21.0%,10.3%,9.5%;真菌共检出5属,其中优势菌属为曲霉属、青霉属、毛霉属、根霉属,分别占总真菌的42.0%,36.0 %,4.4%,4.2%。

There is a space between a peribacteroid membrane and a bacteroid cell wall, the size of spaces is variable in different bacteroids or the different parts of a bacteroid.

细菌周膜与细菌细胞壁之间有一个环形腔隙,腔的大小随细菌细菌部位不同而异。

Abstract] objective to evaluate the effect of regulating bloodmobile sterilization management.methods making a statistical contrast to the number of bacterial in the air flow,on object surface in the bloodmobile car before and after regulating sterilization management.results before regulating management,the total excess rate of bacteria in the air is 41.67% while the rate of object surface is 20.83% in the car.after regulating management,the total excess rate of bacteria in the air is 4.17% while the rate of object surface is 0.00%.the differences were significant.conclusion to realize regulative and effective sterilization management,consolidate sterilization measures,guarantee the quality of blood and the safety of blood collection is of important significance.

摘要] 目的评价规范流动采血车消毒灭菌管理的效果。方法对流动采血车内的空气、物表在规范消毒灭菌管理前后细菌培养数量的统计对比。结果规范管理前车内空气细菌总数超标率达41.67%,物表达20.83%。而规范管理后车内空气细菌超标率是4.17%,物表细菌超标率是0,差异有统计学意义。结论对流动采血车实施规范有效的消毒灭菌管理,务实消毒灭菌措施,对保证血液质量和采血安全具有重要的意义。

Ascites syndromes disease is a common and ubiquitous disease in the industrial culture with turbot, which often results in turbot's massive mortalities. In June 2006, an outbreak of ascites syndromes disease of the cultured turbot Scophthalmus maximus occurred in an indoor farm in Shandong Province of China Through the isolation, purification as well as cultivation of the bacteria in the diseased fish, and by tracing the bacteria in the well water, rearing pond water along with the feeding trash fish, it was found that the bacterium RF-1 which existed in the trash fish was similar to the bacterium FS-1 in the diseased turbot; artificial infection test demonstrated that the bacteria RF-1 and FS-1 had the same infection symptoms for healthy turbots.

腹水病是工厂化养殖大菱鲆的一种常见病和多发病,常造成鱼类大规模的死亡。2006年6月山东某大菱鲆Scophthalmus maximus养殖场发生大规模腹水病,通过对病鱼细菌的分离、纯化和培养,对养殖井水、养殖池水和所用饵料小杂鱼进行细菌追踪调查,将从该养殖场所用小杂鱼中分离得到的细菌RF-1与鱼体分离的细菌FS-1进行比较,并对其进行人工感染试验,结果证明这2株菌对健康大菱鲆都具有很强的致病性,而且感染症状完全一致。

Ascites syndromes disease is a common and ubiquitous disease in the industrial culture with turbot, which often results in trubot's massive mortalities. In June 2006, an outbreak of ascites syndromes disease of the cultured turbot Scophthalmus maximus occurred in an indoor farm in Shandong Province of China. Through the isolation, purification as well as cultivation of the bacteria in the diseased fish, and by tracing the bacteria in the well water, rearing pond water along with the feeding raw fish, it was found that the bacterium RF-1 which existed in the trash fish was similar to the bacterium FS-1 in the diseased turbot; artificial infection test demonstrated that the bacteria RF-1 and FS-1 had the same infection symptoms for healthy turbots.

腹水病是工厂化养殖大菱鲆的一种常见病和多发病,常造成鱼类大规模的死亡。2006年6月山东某大菱鲆 Scophthalmus maximus 养殖场发生大规模腹水病,通过对病鱼细菌分离、纯化和培养,对养殖井水、养殖池水和所用饵料小杂鱼进行细菌追踪调查,将从该养殖场所用小杂鱼中分离得到的细菌RF-1与鱼体分离的细菌FS-1进行比较,并对其进行人工感染试验,结果证明这2株菌对健康大菱鲆都具有很强的致病性,而且感染症状完全一致。

The results show that though the strain is growing slower in the nitrogen-free medium than in the nitrogen-containing medium, the content of capsular polysaccharides produced by the strain in the nitrogen-free medium is higher than those in the nitrogen-containing medium, the content of capsular polysaccharides produced by the strain in the nitrogen-free medium is higher than those in the nitrogen-containing medium. The capsular polysaccharides produced by the strain in the culture which contains different mineral powders will reach the highest content on the third or the fourth day in its growing period. The strain's capability of releasing potassium from k-feldspar and biotite in the nitrogen-containing medium is higher than that in the nitrogen-free medium because in the nitrogen-free medium, the strain and its production of glucoprotein are less than those in the nitrogen-containing medium.

结果表明,尽管该菌在无氮培养条件下的菌体数量远小于有氮培养条件,但无氮培养条件下所提取的细菌多糖多于在有氮条件下培养所提取的细菌多糖;胶质芽孢杆菌在以添加钾长石粉或黑云母粉制作的无氮培养基中生长可形成大量多糖,采用丙酮法进行细菌培养液多糖提取,发现细菌培养的第3天所提多糖含量最高;胶质芽孢杆菌在以添加钾长石粉或黑云母粉制作的有氮培养基中生长亦可形成较多的多糖,且在细菌培养的第4天所提多糖含量最高;胶质芽孢杆菌在有氮条件下对含钾矿物的释钾率高于在无氮条件下的释钾率,这可能与该菌在有氮条件下生长更快、可产生较多菌体细胞有关。

By suspending cell culture from the callus of P. thugbergii and by fluorescent observation, a new method, which is easy and sensitive to observe cell activity and to test the phytotoxicity of various bacteria, was used. This method can be utilized to study the bacterial toxins. Cultured filtrates of some strains of the nematode-carrying bacteria were bioassayed for their toxicity to suspending cultured cells from P. thugbergii callus. Strong toxic effects were observed by the filtrates of Pseudomonas spp., P. fluorescence, and Sphingomonas spp., these bacteria species are coincide with those in the early stage of pine wilt disease.

本试验使用了黑松愈伤组织的细胞悬浮培养方法,用荧光方法观察各种细菌对植物细胞的毒性测定,这种方法具对细胞的活性观察容易、灵敏度高等特点,证明可用于细菌的毒素研究;对松材线虫携带的细菌的部分菌株,用其培养滤液对黑松愈伤组织的悬浮培养细胞的生物测定初步证明,松材线虫携带的细菌中以假单胞菌、荧光假单胞菌和少动鞘氨醇单胞菌的滤液对黑松细胞具有较强的毒性,这与松树发病早期出现的细菌种类相一致。

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