细胞破碎

An amidase from a strain Bacillus cereus ZJB-07112 was purified to homogeneity by using sonication, anion-exchange chromatography, phenyl-sepharose chromatography.

用一株蜡状芽孢杆菌新菌株ZJB-07112 ( Bacillus cereus ZJB-07112)发酵生产酰胺酶，经超声波细胞破碎、High Q阴离子色谱、Phenyl-Sepharose疏水色谱等步骤获得了凝胶电泳均一的酰胺酶。

A amidase from a strain Bacillus cereus ZJB-07112 was purified to homogeneity by using of the sonication, anion-exchange chromatography, Pheny1-Sepharose chromatography.

用一株蜡状芽孢杆菌新菌株ZJB-07112 (Bacillus cereus ZJB-07112)发酵生产酰胺酶，经超声波细胞破碎、High Q阴离子层析、Phenyl-Sepharose疏水层析等步骤获得了凝胶电泳均一的酰胺酶。

The best selenium-adding time was at 24 hours. When the concentration of sodium selenite was over 12μg/mL it obviously inhibit the conversion of inorganic selenium by yeast. The assimilation rate of selenium by yeast was 62% and the conversion rate was 53%. The cell broken rate with ultrasonic cell pulverizer was about 55%.

啤酒酵母菌的最佳加硒时间为24h，亚硒酸钠浓度大于12μg/mL对啤酒酵母菌转化无机硒有明显抑制作用，啤酒酵母菌对无机硒的摄入率约为62%，转化率约为53%；超生波细胞粉碎仪破碎细胞的破碎率为55%左右。

At present, knowledge of this general structure does not provide a direct guide to methods of cell rupture.

现在所有的关于细胞膜结构的基本知识，并不能为细胞破碎方法提供基本的引导。

In a few others, desired products like steroids can be extracted without rupturing the cells.

还有，产物如类固醇不必通过细胞破碎提取。

However the Espf7 was gene engineering strain of DCase's genes cloned in E.coli.DCase expressed by Espf7 could transform NC-D-pHPG into D-pHPG.But DCase was in cell membrance,Dialysing cell membrance was investigated.

此外还有一菌种Espf7是克隆了DCase基因的大肠杆菌，它能表达DCase活性继续将NC-D-pHPG转化为D-pHPG，可DCase是胞内酶，故需要对其进行细胞破碎才能释放出酶。

However the Espf7 was gene engineering strain of DCases genes cloned in E.coli.DCase expressed by Espf7 could transform NC-D-pHPG into D-pHPG.But DCase was in cell membrance,Dialysing cell membrance was investigated.

此外还有一菌种Espf7是克隆了DCase基因的大肠杆菌，它能表达DCase活性继续将NC-D-pHPG转化为D-pHPG，可DCase是胞内酶，故需要对其进行细胞破碎才能释放出酶。

Microscopical identification and TLC discrimination: After the expanded process, the structure of ginseng changed greatly, starch grain gelated, clustered crystal, trachea, were destroyed, the ginsengs hardy texture become loose; The ginseng and the expanded ginseng have the same chromatography stains,color and Rf values.

显微、TLC鉴定：人参膨化后组织结构发生显著变化，淀粉粒糊化，簇晶、导管、木栓细胞破碎，质地坚实的结构变得蓬松；人参膨化前后TLC的斑点颜色、数目、Rf值均相同。

In passing we should note that cationic detergents with two long chains are often oil soluble, and are implicated in the formation of membrane pores.

过去认为带有两个长尾的阳离子表面活性剂溶于油，和细胞膜孔的形成有关，但是很少用于细胞破碎。

By using the EBA chromatography, however, the desired product can be directly recovered and purified from cell homogenates.

但是，利用膨胀床吸附色谱可以直接从细胞破碎液中分离提取目标产物。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。