筛选

Finally, 30 of actinomycosis of the initial screening and re-screening, to find the biological activity of strain, strain through 16SrDNA activity and biological characteristics of the determination of the detection, a preliminary to determine their taxonomic status.

最后通过对30株放线菌的初步筛选和再次筛选，找到有生物活性的菌株，通过对活性菌株的16SrDNA的测定和生物学特征的检测，初步确定其分类地位。

Two of them are bacillus and gram positive, and the other one is uncertain. All of the three strains are facultative aerobe, and able to tolerant 11.53% of NaCl and 85.3℃. They are adapt to the harsh environment of oil deposit.

本研究确定了一套实验室可行菌种筛选方法，从不同来源筛选出三株异养菌株，其中两株革兰氏反应呈阳性，初步推断为芽孢杆菌属，另一株未定。

To solve these problems, a new AHP method is presented by combining the weight vector rang estimation analytic theory with traditional evaluation index rejecting method.

针对用层次分析法进行评价指标筛选过程中经常出现比较矩阵不一致、相对权重值差别较大等问题，将权重向量区间估计分析理论与传统的评价指标剔除方法相结合，提出了一种新的AHP评价指标筛选方法，并以海底管道工程方案评价指标体系的确定为例进行了实际应用，结果表明该方法具有较强的实用性和可操作性。

These five strains were identified as Bacillus cereus、Acinetobacter calcoaceticu、Pantoea agglomrans、Pseudomonas putida and Pantoea ananas by 16S rDNA gene sequence phylogenetic analysis, respectively. These bacteria species is the first reported in oilseed rape rape.

本研究显示从油菜内生细菌中可筛选到拮抗作用好的菌株，并且内生细菌可产生具有抑菌活性的代谢物，可以作为对多种植物病原真菌具有抑菌活性物质的筛选资源。

In this study, a feedback insensitive anthranilate synthase gene ( ASA2 ) cloned from a tobacco cell line was tested for Agrobacterium mediated transformation of axis tissue of soybean mature embryo, with a tryptophan analogue 5 methyltryptophan (5 MT) as the selective agent.

通过从烟草中克隆的邻氨基苯甲酸合成酶基因(ASA2 )作为筛选标记基因，并采用氨基酸的类似物 5 甲基色氨酸为筛选剂，进行了农杆菌介导的大豆成熟胚尖转化研究。

Then it proposes to construct the venture capital project screening appraisal's procedure and the standard and establish a set of complete assessment method, and has made the case analysis.

本文在对国内外文献做了系统、全面的梳理并回顾风险投资项目筛选评价理论的基础上，提出了风险投资项目筛选评价的程序和标准并建立了一套完整的评价方法，并做了案例分析。

One cDNA clone was identified by immunoscreening from a cDNA expression library prepared from Babesia gibsoni merozoite mRNA and its complete nucleotide sequence was 2108bp in length,including one single open reading frame and encoding a 62ku protein which had high homology with that of apical membrane antigen-1(AMA-1) of other species.

通过免疫筛选法，从构建的犬吉布森巴贝虫cDNA基因表达文库中筛选到1个cDNA克隆，此cDNA核苷酸全长2108bp，包含1个完整的开放阅读框，编码蛋白的分子质量约为62ku。

In a commercial nursery using the peat moss-plug system, these three strains also showed their ability to promote seedling growth following either seed bacterization or peat moss drenching, and their populations in peat moss maintained at high levels (except strain RS65 for 30 min seed soaking ) within 21 days after seeding. The above 14 strains that were capable of promoting tomato growth were tested for their ability to control bacterial wilt of tomato.

自台湾中部不同地区数种作物分离得到之396株根栖细菌，被覆於番茄种子后，利用套袋塑胶培养皿之系统及含泥炭土栽培介质之穴盘系统於生长箱筛选，共有 14株菌株在重复筛选过程中均能提高种子发芽率及促进根生长之效果，其中RS4、RS65及RS70三菌株表现最佳，可增加根长度、茎长度、植株鲜重及乾重(RS65菌株除外)。

To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中，苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变，并筛选在自生生长时期、共生生长时期的表型，以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因，我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法，发现lsrA基因主要在根瘤侵染区开始表达，表达时序也在侵染阶段左右，但晚于bacA基因表达；LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体，nifA和fixK基因的转录水平降低，nifH基因的转录被完全阻断，因此LsrA蛋白为根瘤发育所必需，是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因，并研究了它的调节特性。oxyR突变后，苜蓿中华根瘤菌对过氧化氢敏感性提高，适应性降低。

①Location of WD gene in Ch inese: Using pairwise linkage analysis and multipoint linkage analysis method, w e constructed a genetic map of DNA markers within D13q14.2-3 which refined the location of WD gene by restriction fragment length polymorphism and microsatellite polymorphism analysis;②Screen for mutations of WD gene in Chinese people: we detected the structure of 21 exons of WD ge ne in 45 patients from 39 pedigrees by PCR-SSCP(Single strand conformation poly morphism) and PCR-DNA sequencing technology, found a new mutation in exon 5 and nuclcotide sequence analysis showed it is a T insertion. We also conformed the Arg778Leu in exon 8, the highest frequence mutation point in Chinese people, wit h mutation rate 22.8%in total;③Carrier detection and presymptomatic diagnosi s of WD: Based on DNA recombination technology, we peformed successfully the gen e diagnosis in all individuals of 79 families with WD and built up a helpful spe cific enzyme cut method (PCR-Msp1) to detect the carrier and presympomatic patients in Chinese pe ople with WD.

①WD的基因定位研究：通过RFLP及微卫星多态性分析，应用两位点及多位点连锁软件，建立了中国人WD基因在D13q14.2-3区域的精细遗传连锁图谱，从而首次对中国人WD基因进行了精确定位；②WD基因突变研究：应用PCR-SSCP及DNA测序技术，对39个家系45名WD患者进行该致病基因的21个外显子突变筛选，发现WD基因5号外显子存在新的T插入突变，并证实中国人WD基因的突变热点为8号外显子，突变形式为Arg778Leu，其频率为22.8%；③WD的症状前诊断和杂合子检出：应用DNA重组技术对79个家系进行基因诊断，成功地进行了WD的症状前诊断和杂合子检出，并建立了WD的基因筛选的PCR-Msp 1酶切方法。

Life. But the intrinsic way of the Internet by wire confines accessing and using of the network information.

然而，Internet固有的有线连接方式大大限制了人们对网络信息的访问和使用。

I have a strong persuasion that this is true .

我坚信这是正确的。