筛选

The expression of CD59 on the cell membrane was tested by cell immunohistochemistry and flow cytometry. Results pALTER plasmid containing CD59 was cut with restriction enzymes and a 496 bp fragment obtained by electrophoresis, which was complete conformity with the insert.

将两种含有不同突变体的人CD59全长cDNA序列重组pALTER质粒，应用阳离子脂质体导入法与pcDNA共转染CHO细胞，用G418筛选阳性克隆，应用细胞免疫组化及流式细胞仪检测CD59在CHO细胞表面的表达。

Methods: Two recombinant palter plasmid containing whole CDNA sequences of HMCD59 transfected CHO cells by lipofectamine with PCDNA respectively. Then G418 was used to select positive clone.

分别构建两种含有HMCD59全长CDNA序列的重组pALTER质粒，并与pCDNA3质粒，按3：1比例混合后，运用脂质体介导法共转染CHO细胞(编号为HM5-CHO及HM6-CHO)，用新霉素类似物G418初步筛选出阳性克隆。

AIM To study and to compare the efficiency of various panning strategies against phage antibody library.

目的 对多种不同的噬菌体抗体库筛选方法进行比较研究。

This study showed that papaverine test was a simple, safeand reliable screening method for vascular impotence provided the procedure was proper.

作者认为只要掌握罂粟碱试验的正确操作及判断结果的方法，此试验为一种简单、安全且可靠的血管性阳萎的筛选方法

The final product according to the optimization lyophilization curve was qualified. It could be used in industrialized manufacture process for papaverine hydrochloride injection.

筛选出的冻干曲线生产周期短，生产运行成本低，制得成品检验合格，可用于注射用盐酸罂粟碱的工业化生产。

The materials, paprika, cinnamon and chili powder and coffee and sugar were sifted over the floor of my studio.

这些材料，辣椒粉，肉桂，辣椒粉，咖啡和糖的筛选在我的工作室发言。

Linus's Law can be rephrased as ''Debugging is parallelizable''.

经过了这些筛选的人一般都会有可以贡献的真才实料。

The aim was to screen the optimal nutrition formula and environmental condition suitable for growth and propagation of paramecium.

［目的］筛选适宜草履虫生长及繁殖的最佳营养配方及环境条件。

Moreover, the resistance level of the RSC strain was higher than that of the SRC strain, demonstrating that selection with more resistant hosts can accelerate the development of resistance in the parasitoid. Ratios of MFO activity in the SP, SRP and RP strains of P.

以SRP为寄主的RSC品系的抗性水平高于以SP为寄主的SRC品系的抗性水平，表明和抗性较高的寄主同步筛选，寄生蜂的抗性发展更快。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

Methods: Five patients with parkinsonism or dystonia were assigned to general anesthesia using an modified endotracheal tube.

本实验依照人体实验之相关规定进行，五位患有帕金森氏症或肌张力异常的病人接受神经立体定位手术。

If you can benefit from this book, it is our honour.

如果您能从本书获益，这将是我们的荣幸。