筛选

The result implied that resistance mechanism of the mutants and the normal resistant variety was alike at physiological and biochemical level.

说明离体筛选的抗赤霉突变体的生理生化抗性机制与一般抗性品种类似。

The results of this selection will be carried out notary public notary.

此结果进行筛选的公证人公证。

By following GFP expression, pancreas formation was detected at the 18h post-fertilization in the earliest time points between the second bilateral somites, a group of GFP-positive cells located from ventral to the notochord.

通过方便的荧光筛选，我们观察到胰岛在受精后18h开始形成，1－5d后由初始的脊索中线两侧向右迁移。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

RESULTS: BMSCs was pure following density gradient centrifugation and adherence screening method. The third and fifth passage of cells had typical whirlpool-shape. Transmission electron microscope demonstrated that round or oval MSCs possessed large nuclei, big nucleus proportion, a few cellular organ.

结果：①经密度梯度离心法、贴壁筛选法并结合传代方法所得的间充质干细胞很纯，第3代和第5代细胞形态单一，细胞排列具有典型的漩涡状结构。

In our previous STM studies we found that the disruption of the hns gene encoding a histone-like nucleoid structuring protein could enhance biofilm formation of APP.

我们在筛选APP转座突变体库时，发现hns基因缺失突变可显著增强APP血清1型生物被膜的形成，该基因编码一种DNA结合蛋白。

The nucleoprotein gene and glycoprotein gene of rabies virus were amplified by RT-PCR and then cloned into pMD18-T plasmid,respectively.

将N基因和G基因分别克隆入质粒载体pMD18-T后，对筛选的含有N基因和G基因的重组质粒进行了全基因序列测定和分析。

Three pairs of elite cell lines from octoploid triticalespikes were selected for screening experiment of salt-tolerant somaclonal variants under different selection pressures.

从八倍体小黑麦的单、双倍体来自同一植株的成对愈伤组织细胞系中，选择了三对在盐分浓度不同的培养基上进行变异体筛选，并测定耐盐变异体的出现频率。

Two new ways to patch the oddments together and distinguish genuine from false information are reported this week.

本周，科学家报道了两种可以将这些零碎的信息整合起来，同时，能够从错误信息中筛选出真实信息的新方法。

The selection pressure at the translation level is evident in all bacteria based on the analysis of the skew at the three codon positions.

基於三个转录子位置的碱基组态分析，转译层次上的筛选效应存在於所有的细菌当中。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。