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Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区,克隆进PET-32a表达载体,双酶切和PCR鉴定出阳性者,命名为PET-32pgE,转化BL21菌株,挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和,按2%体积转种另一管,培养至OD_(600)为0.6,加IPTG至终浓度1mmol/l,诱导4~6小时,SDS-PAGE和Westernblot分析,证明确实表达并具有免疫反应性。

Acetone and methyl ethyl ketone does have a strong solubility, due to solvent inks are pigment dispersant through a combination of bond and bond formation of throwing power, and too strong solvents will destroy this balance, resulting in pigment dispersion instability precipitation, the ultimate plug nozzle.

丙酮和丁酮确实有很强的溶解性,由于溶剂墨水中的颜料是被分散剂通过键与键的结合形成分散能力的,过强的溶剂会破坏这种平衡,造成颜料分散的不稳定,产生沉淀,最终堵塞喷头。

Unless AQA can obtain demonstration by objective evidence that the full system requirements relating to legal compliance, covering

某些有关法律法规符合性的特殊数据及其他信息进行审核,会导致组织无法进行注册或继续注册,除非AQA获得确实证据

San-Huang, so called Mesima in Japan and Korea, has been considered to be a traditional Chinese and Japanese medicine that was reported to be used as a stypti, both internally and externally, for treating uterine bleeding, and for blood in the urine. The modern researches proved that it is also an effective emmenogogue for amenorrhea and for treating leukorrhea. Extracts from hot water have been experimented to have antitumor and inhibit Sacroma 180 cancer cell abilities. The powdered fruiting body is taken in liquid or in pill form, and preparations can also be applied externally for pains in the abdominal region.

在传统的药用真菌中,桑黄在明代李时珍的「本草纲目」即有记载,性寒,味微苦,能利五脏,宣肠气,排毒气,压丹石,人热发,止血等,在中国及日本有长久的使用历史,现代化科学研究证实桑黄的热水抽出物具有抑制老鼠Sacroma 180肿瘤细胞及一般抗肿瘤的功能,治妇女月经不顺,提高免疫机能及强化T细胞能力,对癌症肿瘤切除后化疗的患者具有相乘的功效且长期服用并不会有任何副作用及毒性产生,这些证据显示「桑黄」确实具有开发潜力。

Of course she was right; for in image after image, in the work of a life time, and most profoundly in his book American Photographs, Walker Evans continues to show us, even now seventy some years later, who we are, how we live, what we value, and most subversively, what we love and what we fear.

确实如此,他的每一张照片,他一生的创作,以及他那影响深远的《美国摄影作品集》,70多年来一直在启发我们思考:我们是谁,我们如何生活,我们珍视什么。其中最具颠覆性的是:我们热爱什么,畏惧什么。他的图片所呈现的景象早已不复旧观,但其所关注的议题尤存。

"We are the most regulated industry in the world,"says Corr.

&医药产业是全世界最具规则性的产业,&Corr说,&坦白讲,也确实应当是这样,假如一个药有问题,我们希望尽早知道。&

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推荐网络例句

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克,果实色泽鲜艳,汁多味浓,甜酸适口,离核仁甜,清末时就驰名陕、甘两省,清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异:红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感,ED90值分别为47.65、71.67和29.17g/hm2;春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感,ED90值分别为96.91、114.20g/hm2;而番茄不敏感。

However, I have an idea.

不过,我有个主意。