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GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株,经筛选后倒置荧光显微镜观察细胞形态及荧光状态,挑取荧光最强的克隆,命名为ES-GFP细胞,进一步扩增培养,观察细胞生长及荧光表达情况,行细胞分化全能性鉴定包括碱性磷酸酶(alkine Phosphtase,AKP)染色、体外胚胎体(embryonic body,EB)形成实验及裸鼠体内成瘤实验。2。

The high expression rates of Cpn-Ag were founded in adventitia of coronary artery atheroma, the Cpn may be one of causational factors for the formation of lesions within the coronary artery atherosclerosis.

Cpn-Ag在冠状动脉瘤外膜下有高表达率,Cpn可能是参与冠状动脉粥样硬化形成损害的原因之一。

However, when macrophage uptaking too much OxLDL, they would die, and they would not digest OxLDL. Furthermore, the abundant death macrophages could accelerate the formation of fatty streak to process to atheroma.

在前人研究中指出巨噬细胞吞噬 OxLDL后将可进一步将其分解,倘若细胞吞噬 OxLDL后即死亡,将无法分解 OxLDL;且大量死亡的巨噬细胞可能加速脂肪条块的形成而演变为动脉粥状瘤。

By the test of clonal growth in soft agar medium , we compared the ability of cloning and proliferating among these three kinds of cells. The cell cycle was analyzed by FCM and morphosis of cells was observed under transmission eletron microscope.

通过细胞生长曲线,比较卵巢癌细胞系HO-8910与8910-p93、8910一pCDNA3.1(转染空载体ncDNA3.l)的增殖情况;通过软琼脂克隆形成试验比较这3种细胞的克隆形成能力和增殖能力;利用流式细胞仪检测这3种细胞的细胞周期的改变情况:利用透射电镜观察转染p93基因的细胞的超微结构变化;利用裸鼠移植瘤实验比较HO七与8910个93在动物体内生长状况。

In this report, immunohistochemistry and in situ hybridization techniques were perfected to examine the expression of bFGF, fibroblast growth factor receptor-I and bFGFmRNA of cultured HEP-2 laryngo-tumor cell and 803 gastro-tumor cell and of the solid tumor tissue formed by implanting the cultured cells into subcutis of nude mice, intending to elucidate the correlation between the expression level and the tumor formation.

本研究建立了免疫组化和原位杂交的检测方法,通过对体外培养的HEP-2喉癌细胞和803胃癌细胞和这两株细胞在裸鼠皮下形成的实体瘤组织中bFGF,纤维细胞生长因子受体-1(fibroblast growth factor receptor-1,FGFR-1)和bFGFmRNA的检测试图揭示三者在体外和体内表达以及与肿瘤形成的关系,从而为这方面的研究提供更多的材料。

Through observing and analy-sing the successive photographs of cutting process taken with the highfrequency camera,it is suggested that the process of built-up edgeformation consists of two stages——base-forming and growing stage.

通过观察和分析高频摄影所得的切削过程连续照片后,提出积屑瘤的形成过程是由形成基础和长大两个阶段组成的。

After treatment with the conditional medium of tumor cells, the number of tubules increased in a dose-dependent manner.

结果:血管生成素-2在神经胶质瘤细胞中的表达强度与瘤组织的分级呈正相关(p.05);肿瘤细胞诱导肿瘤血管形成呈浓度依赖效应(p.05)。

Flow activities inside the half stented aneurysm model were significantly diminished, specifically the pressures and wall shear stresses in the blob were decreased. It can be concluded that half bridged endovascular stents can promote intra-aneurysmal thrombus development and attenuate aneurysm rupture risk, and it is a feasible approach to treat aortic arch aneurysm.

仿真结果表明:半桥支架模型瘤腔内的流动受到明显抑制,特别是局部突起处的压力和壁面切应力大大地减小了,因此,半桥支架可以促进瘤腔内血栓的形成,并能减小动脉瘤破裂的危险,是一种可行的支架治疗动脉瘤的方法。

These include depolarization of the membrane potential in epidermal cells, specific expression of early nodulin genes and changes in the flux of calcium in root hairs, deformation of root hairs, cell division in the root cortex and formation of the nodule primordium.

结瘤因子信号能够诱导豆科植物根毛细胞质膜去极化、离子流动和早期结瘤素基因的表达以及根毛变形、皮层细胞分裂和根瘤原基形成等与共生有关的表型变化。

Result vitamin A and 4HPR fall to Hela cell collect form have apparent inhibition, can restrain Hela cell to be opposite the action causing tumor of naked rat (the curb tumour action of P.01); vitamin A issues visible cell solid by force to shrink at 4HPR(P.01); report lens, nuclear film is screwy, nuclear chromosome gathers agglomerate stand by the wither such as nuclear film to die DNA of cell of cellular feature; by degradation, a collection of illustrative plates of typical " of shape of " a flight of stairs appears in electrophoresis of agaragar candy gel.

结果维生素A和4HPR对Hela细胞集落形成有明显抑制功能,并可抑制Hela细胞对裸鼠的致瘤功能(P.01);维生素A的抑瘤功能强于4HPR(P.01);电镜下可见细胞固缩,核膜扭曲,核染色体聚集成块并靠近核膜等凋亡细胞特征;细胞DNA被降解,在琼脂糖凝胶电泳中呈现典型的&阶梯状&图谱。

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Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克,果实色泽鲜艳,汁多味浓,甜酸适口,离核仁甜,清末时就驰名陕、甘两省,清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异:红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感,ED90值分别为47.65、71.67和29.17g/hm2;春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感,ED90值分别为96.91、114.20g/hm2;而番茄不敏感。

However, I have an idea.

不过,我有个主意。