生物测定法

Using MTT and AO/EB methods, the photosensitization of fullerenols under visible lights with different wave bands were studied.

用MTT法研究了在不同波段可见光和远红外辐照的下的细胞生物效应；用AO/EB荧光染色法测定了富勒醇在可见光辐照下对L02细胞的损伤。

METHODS: A single oral dose of 40 mg rabeprazole capsules or tablet was given to 18 healthy male volunteers in double alterrative periode for an open randomized cross-over test.

18名男性健康志愿者随机分2组，按双周期交叉口服雷贝拉唑的2种制剂，剂量40mg，用高效液相色谱法测定给药后不同时间点血浆中雷贝拉唑的浓度，计算2种制剂药动学参数和相对生物利用度，并评价其生物等效性。

Heilongjiang Forest Botanical Garden, Harbin 150040, Heilongjiang, China Abstract: Using growth rate and hanging drop methods, biological activity of four acetone extracts from Sophora flavescens Ait., Galinsoga parviflora Cav., Typhonium giganteum Engl., and Datura stramonium L.

黑龙江省森林植物园，黑龙江哈尔滨 150040 摘要：采用生长速率法和悬滴法测定了苦参、牛膝菊、独角莲、蔓陀萝4种植物的丙酮提取物对水稻恶苗病菌、稻瘟病菌、玉米小斑病菌、玉米弯孢菌叶斑病菌、番茄叶霉病菌和辣椒根腐病菌的生物活性。

Methods:Forty patients suffering from ulcerous colonitis and 40 normal people were tested by nitrate reductase methods and bistin streptavidin.

采用硝酸还原酶法和生物素链亲和素ELISA法测定40例溃疡性结肠炎患者和40例正常人血清NO和TNFα。

The graft polymerization of acrylonitrile on Crosslinked polyvinyl alcohol microspheres CPVA microspheres was performed by using cerium ammonium sulfate as initiator, and grafted microspheres CPVA-g-PAN were prepared. Subsequently, the grafted PAN was converted into poly via amidoximation reaction with hydroxylamine hydrochloride as reagent, resulting in the functional microspheres CPVA-g-PAO. The chemical structure and physicochemistry characters were adequately characterized with infrared spectrum, scanning electron microscope and determining Zeta potential. The effects of main factors on the amidoximation transform reaction of the grafted PAN were researched with emphasis, and the adsorption property of the functional microspheres CPVA-g-PAO for some biomolecules was examined preliminarily with uric acid as a model biomolecule.

以硫酸铈铵为引发剂，实施了丙烯腈在交联聚乙烯醇微球表面的接枝聚合，制备了接枝微球CPVA-g-PAN，然后以盐酸羟胺为试剂，通过偕胺肟化转变，将接枝的PAN转变为聚偕胺肟，制得了接枝有聚偕胺肟的功能微球CPVA-g-PAO，采用红外光谱法、扫描电子显微镜及Zeta电位测定等法，对功能微球CPVA-g-PAO的化学结构及物理化学特性进行了表征，重点考察了各主要因素对接枝PAN的偕胺肟化转变反应的影响，也以脲酸为模型分子，初探了功能微球CPVA-g-PAO对生物分子的吸附性能。

The ability of magnesium trisilicate to adsorb tetracycline and its relation to pHof the medium have been investigated UV-spectrophotometrically.

本文应用紫外分光光度法研究了体外三硅酸镁吸附四环素的能力及其与介质 pH 的关系；并应用尿药法研究了三硅酸镁及其在表面活性剂和枸橼酸的存在下对四环素吸收的影响；测定了三种不同处方的四环素混悬剂的相对生物利用度并进行了有关参数的统计比较。

Extract and quantitative analysis of biological active ingredients total triterpene s in Suosuo grape.

采用正交实验设计，利用超声法从琐琐葡萄中提取生物活性成分总三萜，比色法测定总三萜的含量。

The results showed that Kaidimycin was chemically neutral, macrocylic polylactones, stable and liable to dissolve into water and pyridine, slight to dissolve into methanol, ethanol and N-butanol, difficult to dissolve into ethyl acetate, acetone, benzene and chloroform. Chemical reaction of functional group of Kaidimycin showed that it had glycoside, amino acid and aldehyde, no reducing sugar, polypeptide and phenol.

凯地菌素属中等极性的、中性抗生素，极性纸层析结果与多烯类抗生素的纸层析结果相似：易溶于水、吡啶，微溶于甲醇、乙醇、正丁醇，不溶于乙酸乙酯、丙酮、苯、三氯化碳；对高温、光照和紫外处理有很好的稳定性；官能团反应试验结果发现，凯地菌素可能含有糖苷键、氨基酸伯氨基和醛基，不含有还原糖、多肽和酚基，高锰酸钾、浓硫酸反应与多烯大环内酯类抗生素的特性相似；并且依据纯品，采用生物法和分光光度法对摇床发酵液进行了效价的测定。

Chapter 3 further studies on the visual science about the theoretical foundation and measuring method of optical biological effect.

论文第三章进一步研究了视觉科学最新成果光生物效应的理论依据和测定方法，视觉功效法是研究中间视觉的有效研究方法，反应时间表征了中间视觉和光生物效应的总视觉效果和人眼真实的视觉功效。

METHODS: An open, randomized, twoperiod crossover study was conducted in 18 healthy volunteers. Concentrations of gatifloxacin in plasma were assayed by HPLC after a single oral dose of 400 mg of the tested capsules or the referenced tablets. The main pharmacokinetic parameters ［AUC（0-24h）, Cmax and Tmax］ were analyzed by two onesided t test. RESULTS: The concentrationtime curve after medication conformed to onecompartment open model with a first order absorption.

采用双周期随机交叉、自身对照试验设计法，将18例男性健康志愿者随机分为两组，分别单剂量口服甲磺酸加替沙星胶囊400 mg和甲磺酸加替沙星片400 mg，经1 w清洗期后交叉换药，以高效液相色谱法测定受试者服药后血浆中加替沙星的经时浓度，计算加替沙星药动学参数，以双单侧t检验统计法比较两种甲磺酸加替沙星制剂是否具有生物等效性。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。