生物测定法

Methods SH-SY5Y cells, a human neuroblastoma cell line, were incubated with different concentrations of fluoride for 48 hr and somle of them were treated with vitamin E precedently. The functional situation of cells was measured by MTT method; lipid peroxidation was detected by High-Performance Liquid Chromatography; phospholipid was separated by a Silica SepPak cartridge and neutral lipids by HPLC.

体外培养SH-SY5Y人脑神经母细胞瘤细胞，在培养液中加入不同浓度的氟化物或加入抗氧化剂，培养48h后用测定细胞MTT的方法来了解细胞的损伤程度，用高效液相色谱法分离和测定培养液中脂质过氧化物水平，用过柱和比色法测定细胞生物膜磷脂含量，用高效液相色谱法测定细胞生物膜辅酶Q和胆固醇含量。

Measure the inhibition by agar piece method. After puddling treatment raise seedlings and dipping root pot culture test, get the growth promoting effect to the strawberry and measure the strawberry leaves and root's PPO activity. so as to get the induced endurance.

采用琼脂块法、拌土育苗和蘸根盆栽生物试验及PPO活性测定法研究供试放线菌对病原菌的皿内拮抗作用、对草莓的促生作用及诱导抗性。

The following contents have been studied comprehensively: the method of assimilable organic carbon measure; the biological stability of raw water in Yangtze Delta; the removal characteristics of AOC by purification technology and pr...

在熟练掌握和运用AOC生物修订法的基础上，对其进行了简化和改进，以100mL具塞玻璃锥形瓶代替45mL硅硼酸盐管形瓶，将测试水样量由40mL增加到100mL，将9个平行水样减少为6个，降低了AOC测定工作强度，有利于推广应用。

Results When cellulase and pectinase compound,amlyse and diastatic enzyme were added in order in the method of stepwise biocatalysis,98% diosgenin from plants was extracted.IR spectrum of the product was in accordance with that of reference substance and the purity of it is more than 95% according to its HPLC chromatogram.

结果利用阶梯生物催化法，当纤维素酶和果胶酶复合酶制剂、淀粉酶以及糖化酶按顺序依次加入时，能够将植物中98%的薯蓣皂苷元提取出来，其产品的红外谱图与对照品谱图相吻合，经HPLC谱测定薯蓣皂苷元质量分数95%以上。

AbstractSerum prostate specific antigen-the biotin-avidin enzym immunoassaywas estab-lished and used in clinical practice.

作者建立了血清前列腺特异抗原的生物素－亲和素酶联免疫测定法并用于临床。

Chlorpyrifos mehtyl with different dosages of 3,5,10 mg/kg compared with malathion 15 mg/kg in grain silo,each contented 40 thousand kilograms of rice was studied.To be sprayed by machine when loading to silo and to be observed two years through all experiment,the effective control to sitophilus zeamais of chlorpyrifos mehtyl 5 mg/kg can be protected more than six months,and 10 mg/kg over one year.

报道了甲基毒死碑(化学名称为二甲基O-3，5，6-三氯-2-吡啶基硫逐磷酸酯)3、5、10 mg/kg不同剂量，以防虫磷15 mg/kg作比较，在立筒库星仓内，每筒40000 kg稻谷，机械喷雾法处理，储藏二年，经生物测定对玉米象药效，甲基毒死蜱5 mg/kg可达半年以上，10 mg/kg可达一年以上防护期。

Study the bioactivity of the n-HA/PA66 composite and the effects it would be to body's metabolism of calcium and phosphorus ion in vivo.(3) Study the osteo-conductivity and the ability to repair bone defect of the porous n-HA/PA66 composite and the feasibility use it as the scaffold of bone tissue engineering. Objects and Methods as follows: 1.To evaluate the biocompatability of nano-hydroxyapatite crystals and polyamide composite (n-HA/PA66) with the L929 cells.To proceed the morphological observation and take pictures of L929 cells after 1d,2d,4d,and 7d of co-cultured with extract of n-HA/PA66 ,and direct contact with n-HA/PA66.To determine light absorbtion value of every hole under 500 nm with enzyme linked immunity instrument after 1 d,2 d,4 d,and 7 d of contact of n-HA/PA66 extract with L929 cells,and direct contact with n-HA/PA66.In the meanwhile calculate the relative multiplication rate of cells,and evaluate them by six degree tests for cytotoxicity. To investigate the acute and chronic toxic reaction on the whole body induced by the new nano-hydroapatite crystals and polyamide composite(n-HA/PA66)after implanting in vivo and its effects on partial constitution of animal organs after implanting in vivo,and evaluate the potential and degree of subcuticular stimulation reaction.

本实验主要由以下三部分组成：一、n-HA/PA66 复合材料在动物体内、体外的生物相容性及生物安全性评价二、n-HA/PA66 复合材料植入动物体内的生物活性及近期对机体钙、磷代谢影响的实验研究三、网孔 n-HA/PA66 复合材料作为支架修复兔桡骨节段缺损的动物实验研究主要研究目标及方法如下：参照 GB/T16886.5-1997-ISO 10993-5：1992《医疗器械生物学评价细胞毒性试验体外法》之评价标准和要求，采用规定的 L929 细胞(小鼠结缔组织成纤维细胞)，分别经直接接触和材料浸提液与细胞共培养等方式对 n-HA/PA66 复合材料进行细胞毒性测试，采用细胞形态观察法观察两种细胞各组在 24h、48h、72h、5 天后各时相点的细胞形态学变化，并在显微镜下照相，从而对细胞与材料的生物相容性进行定性评价；同时采用细胞生长抑制法，以酶标仪定量测定评价各组 1，2，4，7 天 L929 细胞的相对增殖率，以定量测定并判别材料对细胞的毒性程度。

Domestica in various habitats to dichlorvos and propoxur. Results The bioassay results showed that 4 strains of M.

采用微量点滴法进行生物测定，同时采用AChE检测不同生境家蝇对敌敌畏和残杀威的抗性水平。

Methods:(1) bFGF was loaded with PLGA, PELA and gelatin microspheres. The pharmac characteristics of particle size distribution, loading efficiency, entrapment efficiency, bFGF release kinetics were evaluated with scanning electronic microscope, MTT and ELISA respectively. The prescription with the best pharmac characteristics and the highest bioactivity of bFGF was chosen for the next step.

（1）制备PLGA，PELA和明胶微球，通过扫描电镜和激光粒度分布仪测定其粒径分布；ELISA法测定微球溶出度，包封率，bFGF释出动力学；MTT法测定微球释出的bFGF的生物活性，筛选出一种具有较好药剂学性质和较高bFGF生物活性的微球载体材料。

Using dual energy X-ray bone density instrument XR-36 model to detect the bone mineral density of the left femur of the rats. Using the strain test system, we examined the biomechanics index according the tension and three-spot bending test; we also examined the bone calcium of the lumbar vertebra through plasma emission spectroscopy . We examined the serum calcium and magnesium by using the nomal biomechanics method,and examined the serum acid phosphatase and osteocalcin by using the Elisa method.

使用NORLAND公司XR-36型双能X线骨密度仪，采用小动物骨密度测定软件，测定大鼠左后肢股骨的骨密度；使用应变测试系统，按股骨拉伸和三点弯曲试验，测定股骨生物力学指标；以等离子体发射光谱法测定腰椎骨骨钙含量；以常规生化法测定血清钙、镁含量及血清酸性磷酸酶含量；以Elisa法测定骨钙素含量。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。