生物测定法

After hybridization of two DNA probes with target DNA, EDTA-〓 and β-diketone come close to each other, and an EDTA-〓-β-diketonate ternary complex having strong and long-lived fluorescence was formed, thus the target DNA was detected sensitively with a detection limit of 6 pM (0.6 fmol per assay) by time-resolved mode.

系统研究了使用EDTA-〓为标记物的石墨炉原子吸收光谱免疫分析法，并将之用于人血清中AFP和水中BSM的测定。该法中使用了EDTA-〓标记SA及生物素标记抗体或抗原，在96孔板中的免疫反应结束后，利用稀硝酸将反应产物分解，然后用石墨炉原子吸收光谱法对免疫反应产物进行测定。

Machinable bioactive glass-ceramics containing mica and fluorapatite were prepared by high-temperature melting. The glass-ceramics were analyzed by means of X-ray diffractometry, scanning electron microscopy with energy dispersive spectrometer and Fourier transform infrared reflection spectrometry. The bending strength, hardness and fracture toughness of the materials were measured.

摘 要：采用高温熔铸法制备可加工生物活性云母/氟磷灰石玻璃陶瓷；用X射线衍射、扫描电镜和傅里叶变换红外光谱分析等手段对材料的相组成和微观形貌进行研究，测定材料的抗弯强度、硬度和断裂韧性等力学性能；采用体外模拟体液浸泡法测试材料的生物活性。

Machinable bioactive glass-ceramics containing mica and fluorapatite were prepared byhigh-temperature melting. The glass-ceramics were analyzed by means of X-ray diffractometry, scanning electron microscopy with energy dispersive spectrometer and Fourier transform infrared reflection spectrometry. The bending strength, hardness and fracture toughness of the materials were measured.

摘 要：采用高温熔铸法制备可加工生物活性云母/氟磷灰石玻璃陶瓷；用X射线衍射、扫描电镜和傅里叶变换红外光谱分析等手段对材料的相组成和微观形貌进行研究，测定材料的抗弯强度、硬度和断裂韧性等力学性能；采用体外模拟体液浸泡法测试材料的生物活性。

According to the bone apposition events at the interface, we measured the osteoblast attachment rate with the hemocytometer, the osteoblast growth curve with modified MTT method, the ALPase activity and protein content of cellular layers with modified kinetic method and modified Coomassie'method respectively, and osteocalain content in the cell medium with RIA , at different levels of cell responses, as osteocompatibility indexs.

根据骨内种植体界面的骨愈合过程，分别从成骨细胞反应的不同层次选择了细胞贴壁率、细胞生长曲线、细胞层ALPase活性和蛋白质含量以及细胞培养液中骨钙素的含量作为体外评价材料骨性生物相容性的指标。采用细胞计数板计数法测定细胞贴壁率，采用改良MTT法测定细胞生长曲线。

In this paper, 13 lakes and 2 large-scale reservoirs in Wuhan were investigated, the makeup of species , dominance species and standing biomass of plankton, benthon, hydrophyte, fish and et al were carried out. And the bio-indicate method , diversity index are used to evaluate the water body.

本文对武汉市13个湖泊及2个大型水库浮游生物、底栖动物、大型水生植物、鱼类及其它生物的种类组成、优势种的鉴定及现存量的测定结果进行了分析，运用指示生物法、种多样性指数法对所调查水体进行了水质评价。

The sample was isolated by sephadex G-25 gel filtration column after concentration. Then bioactivity was assayed by Candida albicans. The chemical components were isolated and identified by GC-MS. GC determined the relative percentage content of the extract, nine kinds of components were separated, identified and accounted for about 82.12%, the major compound was propenyl methyl disulfide.

浓缩后的样品通过Sephadex G-25凝胶柱分离提纯其中的有效成分，以白色念球菌进行生物活性测定，并用气相色谱-质谱法对其化学成分进行分离鉴定，GC法测定各种化合物的相对百分含量，共鉴定了9种成分，约占总提取物的82.12％，其中，主要物质是甲基丙烯基二硫化物。

In this study, aerobic biodegradability of 26 different kinds of dyes was tested, using static flask screening test, Warburg respirometer and semi—continuous activated sludge system; then biodegradability of 22 of the dyes under anaerobic condition was investigated by batch and continuous—flow anaerobic digesters; anaerobic metabolic substances of some dyes were analysed by several methods and the pathway of anaerobic degradation of C.

本文用静置烧瓶筛选试验法、瓦勃氏呼吸仪试验法和半连续活性污泥法对26种不同化学结构类型的染料的好氧生物降解性能进行了测定评价；对其中22种染料用间歇厌氧消化反应器和连续流厌氧反应器进行了厌氧生物降解性能的试验，采用多种仪器对部分染料的厌氧降解产物进行检测，对1种蒽醌染料的厌氧降解途径作了推测。

Applications of gas chromatography in the research of biodiesel processing are reviewed with 27 references,including the analysis of fatty acid methyl esters in the reaction products and final biodiesel,the determination of mono-, di-and tri- glycerides ,the contents and distribution of free fatty acids,and the determination of trace methanol in biodiesel.

综述了气相色谱法在生物柴油生产工艺研究中的应用，包括反应产物和生物柴油产品中脂肪酸甲酯含量和分布的测定，单脂肪酸甘油酯、二脂肪酸甘油酯和三脂肪酸甘油酯含量的测定，游离脂肪酸含量的测定以及微量甲醇含量的测定等。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

METHODS Dinitrofluorobenzene was used to induce the contact dermatitis in mouse ear, rhIL-11 0.375-1.5 mgkg^(-1)d^(-1) sc for 10 d, the degree of skin inflammatory reaction was observed. At the same time, the serum level of tumor necrosis factor-α and interleukin-6(IL-6) was measured by radioimmunoassay; the serum level of nitrogen monoxidum was detected by biochemical method and the expression of intercellular adhesion molecule-1(ICAM-1) was detected by immunohistochemical method.

采用2，4-二硝基氟苯致小鼠耳部皮肤接触性皮炎试验模型，观察sc rhIL-11 0.375~1.5mgkg^(-1)d^(-1)，连续10d后皮肤炎症反应程度，同时采用放射免疫分析法测定血清中肿瘤坏死因子-α和白细胞介素-6(IL-6)的水平，采用生物化学检测法测定血清一氧化氮的水平，采用免疫组化法测定皮肤中细胞间粘附分子(ICAM-1)的表达。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。