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Antioxidants of 100 mg/L PVP, 100 mg/L Vc and sorbent of 500 mg/L AC can control browning efficiently. PVP and Vc restrain callus growth, whenas AC can stimulate it.

5mg/L GA3为组培苗的最佳生根培养基,生根率达100%;一定浓度的PVP,Vc或者AC能有效控制褐变现象的发生。

For the bud directly differentiation , the suitable culture medium was MS+BA 2mg/L +NAA 0.2mg/L or MS+TDZ0.01mg/L +NAA0.2 mg/L, of which the induction rate was 88% and 100% respectively. An efficient tissue culture system has been developed with the bud of mature seed of Aesculus hippocastanum L. as explants . Buds were induced from 2cm high young plantlet cultured on MS medium supplemented with 0.6 mg/L 6-BA plus 0.1mg/L or 0.4~0.6mg/L ZT plus 0.1 mg/L NAA for 15 d, and the induction rate was 100%, the mean No. of buds was 35.7; The combination of MS+0.2mg/L 6-BA +0.1mg/L NAA + 10mg/L AD was the suitable culture medium for elongation of the buds.

以欧洲七叶树成熟种子的胚芽进行离体培养和快速繁殖,结果表明:高约2cm的无菌苗在MS+0.6mg/L 6-BA+0.1 mg/L NAA或MS+0.4~0.6mg/L ZT+0.1 mg/L NAA培养基上培养15 d左右可诱导出不定芽,分化频率为100%,平均每株产芽35.7个;MS+0.2mg/L 6-BA+0.1mg/L NAA+10mg/L AD培养基有利于芽伸长;生根培养基为1/2MS+0.4 mg/L NAA+0.2mg/L IBA时,生根率可达75%。

In addition, the time of buds induction directly from leaf is shorter than those from calli.

并在时间上,从芽的长势、芽的生根情况进行比较,结果表明:直接诱导的不定芽比诱导愈伤组织分化的不定芽所需的时间短、长势好、生根快。

In this work, the effects of culture factors ( MET, IBA, culture medium and culturing in dark) on root formation were studied in tissue culture of Celastrus angulatus. The results showed that the root induction was significantly affected by duration of culture in dark, while other factors were least effective.

探讨了培养因子对诱导苦皮藤试管苗生根的作用,采用正交试验设计法测试了苦皮藤生根关键因素多效唑、吲哚丁酸、暗培养及培养基中盐浓度的效应。

In the cuttage of rose ,five stromata were adopted in order to study their influence on the growth of the branches. The result of the experiment shows that vermiculite can ensure the highest survival rate. In the experiment, epicormic branches are treated with IBA at different concentration. The result shows that 200mg/L IBA can also promote the epicormic branch to root. The rooting rate is 91.0%. Temperature plays an important role in rose cuttage.

研究了5种不同基质对多头玫瑰插穗扦插繁殖的影响,结果表明:不同基质扦插成活率不同,以蛭石最好;在试验中,用吲哚丁酸IBA不同浓度溶液处理扦穗,结果表明: 200mg/L吲哚丁酸IBA对多头玫瑰扦插生根有很大的促进作用,生根率为91%;温度对多头玫瑰的扦插也有很大的影响。

The inducing rate was 94.5%; the optimal medium for the inducting rate of fasciculated shoot was MS+1.0mg/L6-BA+0.5mg/LNAA+stem section with bud, The inducing rate was 37.5%.

诱导愈伤组织的最佳组合为MS+1.0mg/L BA+1.0mg/L NAA+0.5mg/L 2,4-D+叶片,其诱导率为94.5%;诱导丛生芽的最佳组合为MS+1.0mg/L 6-BA+0.5mg/L NAA+带芽茎段,其诱导率为37.5%,增殖培养中,以MS+1.0mg/L BA+0.05mg/L NAA效果最好,增殖率为460%,当株高在2cm以上时,转入1/2MS+0.8mg/L IBA+0.2mg/L NAA生根培养基上,接种30d后,生根率可达90%,移栽成活率达92%。

Nonetheless, the result showed that Pinus, Ginkgo, Cycas form a monophyletic group, which is sistered to angiosperms. Together, they from a clade that is sistered to Gnetales.

邻近连接法和贝叶氏导出氏分析结果将松叶蕨、瓶尔小草,和生根卷柏的matK序列一起形成一个单源群,同属石松类的过山龙与生根卷柏序列并未形成姐妹群。

Effects of ABT rooting powder No.1 (ABT1),α-Naphthaleneacetic acid and Indolebutyric acid on the rooting rates, biomass and taxol contents of Taxus chinensis var. mairei cuttings were studied in this experiment.

以清水处理为对照,使用不同激素(ABT1号生根粉、NAA、IBA)处理南方红豆杉插穗,从而研究不同激素处理对南方红豆杉扦插苗生根率、生物量及紫杉醇含量的影响。

We found a clear trnK5'-matK-trnK3' structure in Ophioglossum petiolatum, Lycopodiella cernua and Selaginella doederleinii. PCR with degenerate primers failed to amplify any trnK or matK fragments from other ferns or allies. However, dot blot hybridization showed distinct signals in these plant failed to amplify matK fragments.

本论文鉴定原始陆生植物其他未被检视的重要类群中叶绿体matK基因的存在,结果发现瓶尔小草、过山龙、生根卷柏都有trnK5'-matK-trnK3'的结构,以RT-PCR侦测matK表现时,瓶尔小草和过山龙都有侦测到讯号,但生根卷柏却没有,显示matK的表现在原始陆生植物似乎不是那麼一致。

The secondary root number per plant of Yumai No 49,a mi d-s trength quality wheat,is the smallest among the three varieties except the winte r stage but its root system quality is the best;the intensity of reducing TTC an d the activity of SOD of fresh wheat root are the highest during the period fro m winter stage to flag leaf stage.Since flag leaf stage,its physiological charac ters are weaker and have bigger variation during whole stage.The secondary root number per plant of Yumai No 50,a soft quality wheat,is the biggest except duri ng winter stage,and its dry weight of root of single plant is the heaviest in ev ery stage.Before 19th,March,its intensity of reducing TTC is the weakest and dec reases abruptly after flag leaf stage,but its activity of SOD decreases slowly.R oot dry weight per plant of Yumai No 34,a high quality wheat,is the smallest amo ng the three varieties and its root system quality is the weakest.Before jointin g stage,the intensity of reducing TTC is stronger,but after that,it decreases sl owly though it is smaller,and the activity of SOD decreases slowly in every stag e.

中筋小麦豫麦49号除越冬初期外,其它各时期单株次生根条数均为最少,单株根干重介于豫麦34号及豫麦50号之间,单根质量高;越冬初期至挑旗期,根系活力、根中SOD活性均强于其它两个品种,挑旗后,根系生理活性较小,整个生育期根系生理活性变化幅度大;弱筋小麦豫麦50号除越冬初期外,其它各时期单株次生根条数均最多,单株根干重各时期均为最大;拔节前根系活力最弱,挑旗后根系活力下降幅度较大,根中SOD活性较强且各时期变化幅度不大;强筋小麦豫麦34号,单株根干重最小,根系质量最差;拔节前根系活力较强,拔节后根系活动较弱,下降速度缓慢,根中SOD活性各时期变化幅度较小。

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