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Differentiation rate, Callus induction rate and and Root Induction rate individual was up to 100%.

确定了筛选欧美杨108转化体及选择生根培养时的抗生素浓度。

IBA and IAA could promote each other when they were used together in medium QL.

IBA与IAA在QL培养基中配合使用对诱导生根有增效作用。

Nigra during the experiment. Successfully established the transformation procedure Agrobacterium-mediated of Populus simonii×P nigra: cut vigorous leaves to 2-3 patches through the main vein after growing about 20 days, pre-culture time was 3~4days;centrifuge Agrobacterium when being developped to the logarithm growth timeOD_(600=0.5, then dilute Agrobacterium to the concentration of OD_(600)=0.3-0.4 with MS liquid culture medium or asepsis distilled water for infection;infection time was 6-15 minute;after being co-cultured 3 days, inoculate on the selecting medium to be filtrated in succession during inducing unsureness buds and rooting with Kanamycin(containing Kanamycin 40mg/L and Cefazolin Sodium 700mg/L).

成功摸索出农杆菌介导的小黑杨遗传转化体系:选取生长20d左右的健壮的小黑杨组培苗叶片,通过主叶脉剪2~3下,预培养3~4d;将培养至对数生长期的农杆菌OD_(600=0.5离心后用液体MS培养基或无菌蒸馏水稀释成OD_(600)=0.3~0.4浓度,进行浸染;侵染时间为6~15min;共培养3d后,转入到筛选培养基中进行诱导不定芽及生根阶段的卡那霉素连续筛选(含有40mg/L卡那霉素和700mg/L的头孢唑林钠)。

The amount of total corn root decreased with the increase of average soil BD.

玉米总根量和气生根数量随着整个土层平均容重的增加而降低。

: Colossians 2:7 Rooted and built up in him, and

和合本:西 2:7 在他里面生根建造,信心坚固,正如你们所领的教训,感谢的心也更增长了。

Nevski in vitro and selected several favorable media.Germ-free plants were produced with germination on MS medium. Callus was induced with B5+1.5 mg.L-1 2,4-D+0.5 mg.L-1 NAA,with leaf sheath and hypocotyls as explants.

Nevski种子消毒并在MS培养基上萌发获得无菌苗,以幼苗的叶鞘和胚轴为外植体诱导愈伤组织,经继代后进一步诱导不定芽及生根

Dreamed of sending millions of Italian settlers to Italian East Africa and there were high hopes of turning the area into an economic asset.

而墨索里尼欲把数以百万计的移民者送到东非的殖民地中落地生根,以取得在东非的丰厚经济利润。

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Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来,英国,在此地早期居住的是凯尔特人,已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。

The almost sure central limit theorem is a pop topic of the probability research in recent years,because it has many actual applications in the random analogue.

中文摘要:几乎处处中心极限定理是近几十年概率论研究的一个热门话题。它之所以引起人们的注意是由于它在随机模拟方面的实际应用参见Fisher