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Cultured in MS medium with different concentration of calcium, explants of Luffa Cylindrica, which could not generate root in usual medium, generated roots with enhanced rates and amounts according to the concentration of supplemented calcium. Moreover, some explants show transformed root from either shoot or flower bud.

在正常Ca2+浓度下,仅激素处理不能生根的丝瓜外植体,处以不同Ca2+浓度处理时,随Ca2+浓度升高,外植体生根数明显加快并增多,且有少量植株叶腋处异化生根,在一定程度上改变了腋芽的发育方向,这说明Ca2+改变了卷须芽或花芽的发育方向,使其发育为根。

According to the root type division the Chinese Dwarf cherry belongs to the inductive type of root primitive body,From above several aspects we can see that the mechanism of soft-wood cutting is : Under the condition of without root primordium ,the root primordium appear from cambium by using rooting accelerator under the condition of well temperature ,humidity ,and light, then the root primordium breaks through the cerebral cortex, finally extends to outside .

从以上几个方面我们可以看出,欧李嫩枝扦插生根繁殖的机理为:欧李嫩枝在无根原体存在的情况下,经过生根剂的处理在合理的温度、湿度及光照条件下,由其形成层部位产生根原体,随后根原体突破皮层,伸向外部。

A quick DNA extraction technique which was fitting for maize variety identification and establishing DNA fingerprinting pool was explored ,and the technique was well applied in the following tissues for DNA extraction : embryo, endosperm; radicel, coleoptile , hypocotyl from germinated 1~3 days material .

本实验探索了适用于玉米品种鉴定及DNA指纹库构建的玉米品种DNA快速煮沸法,用快速煮沸法提取了玉米种子胚、胚乳;水培初生根、侧根;种子出苗后的初生根、侧根、胚芽鞘、胚轴、叶片等组织DNA,结果表明,种子胚、胚乳组织;水培2~4d水培初生根、侧根组织;出苗1~5d的胚芽鞘、胚轴组织用快速煮沸法提取DNA效果较好。

This article discussed preventing carnation rottenness, enhancing survival of carnation cutrage and cutting cost of carnation production. We carried out contrastive experiments with carnation cuttage which applied five bactericides added rootone ABT and studied on effect of bactericide to antisepsis of cuttage rootage.

文章对防止香石竹在扦插过程中根茎部位的腐烂,提高香石竹扦插成活率,降低香石竹切花的生产成本,采用常见五种杀菌剂分别加入生根粉ABT中进行香石竹扦插生根防腐的对比试验,研究杀菌剂对扦插生根的防腐作用。

In the test with double factors design, the effects of different combinations of medium and rooting agent on the rotting of Photinia frasery cuttings were studied and suitable cutting medium and concentration of rooting agent under full light atomizing conditions were screened out.

方法]采用双因素设计,通过田间试验研究不同基质和生根剂组合对红叶石楠扦插生根的影响,筛选出全光照弥雾条件下适宜的扦插基质和生根剂浓度。

In the test with double factors design, the effects of different combinations of medium and rooting agent on the rotting of Photinia frasery cuttings were studied and suitable cutting medium and concentration of rooting agent under full light atomizing conditions were screened out.

咱方法暂采用双因素设计,通过田间试验研究不同基质和生根剂组合对红叶石楠扦插生根的影响,筛选出全光照弥雾条件下适宜的扦插基质和生根剂浓度。

The germination test of Sporocarps of Microlepia hancei prantl had been done, the callus can be induced from the delicate stem of Microlepia hancei prantl; The inducement and proliferation medium of callus was MS+6-BA2 mg/L+NAA0.2 mg/L;the optimal combination of hormones of buds differentiation was MS+6-BA 0.4 mg/L; the effect of MS medium in radication culture was better than 1/2MS, the roots of plants were all efficiently induced in MS medium without reference to adding IBA、NAA or IBA+NAA, the rate of radication was high; the effect of seedling cultured in the mixture of perlite and turf soil was better than perlite、garden soil and river sands.the mixture of perlite and turf soil has the excellences such as ventilate、water conservation 、nourishment and so on,the survival rate was higher and seedling substance was also hale.

试验进行了华南鳞盖蕨孢子果的萌发试验,并利用幼嫩茎段进行诱导愈伤组织;愈伤组织的诱导与增殖培养基为MS+6-BA2 mg/L+NAA0.2 mg/L;其芽分化最佳激素组合为MS+6-BA 0.4 mg/L;生根培养中MS基本培养基比1/2MS效果好,MS基本培养基不论附加生长素IBA、NAA或IBA+NAA,均能有效地诱导植株生根生根率高;假植以珍珠岩和泥炭土的混合物为基质效果优于珍珠岩、菜园土和河沙,具有通气、保水、营养等优点,假植成活率高,苗势健壮。

Results showed that B3(IBA+NAA, 300mg/L+300mg/L) was the best treatment in quantity and length of new roots, rate of radication and survival, distributing of roots length; Treatment B4(IBA+NAA, 500mg/L+500mg/L) took the second place; The following treatments in turn was D3 (NAA+VB1+cane sugar, 100mg/L+80mg/L+2%), D4(NAA+VB1+cane sugar, 300mg/L+80mg/L+2%), B2(IBA+NAA, 100mg/L+100mg/L), B1(IBA+NAA, 50mg/L+50mg/L).

结果表明:在生根数量,生根长度,根长分布及成活率和生根率上以处理B3(IBA+NAA,300mg/L+300mg/L)最好,其次是处理B4(IBA+NAA,500mg/L+500mg/L)。然后是处理D3(萘乙酸+维生素B1+蔗糖,100mg/L+80mg/L+2%)、D4(萘乙酸+维生素B1+蔗糖,300mg/L+80mg/L+2%)、B2(IBA+NAA,100mg/L+100mg/L)、B1(IBA+NAA,50mg/L+50mg/L)。

Somatic hybrids grew weakly and took roots hard. By continuous subculture to facilitate to take roots using rhizogenic medium, the regenerants was transplanted on the cropland and backcrossed using cultivar wheat pollen.

体细胞杂种生长势差,生根困难,通过不断地使用生根培养基促进生根,然后移栽到大田中,利用不同播期的小麦花粉回交杂种,获得回交后代。

Semi lignified tresses were cuttinged separately under seven treatments with different proportion of perlite, vermiculite and turf, at 22℃ temperature.

扦插快繁用珍珠岩、蛭石、泥炭按不同比例设计7个处理,在平均温度22℃条件下,用半木质化枝条进行绣球扦插,统计插穗的生根率、生根条数和生根长度。

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