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Plasma urokinaseantigen,tissue plasminogen activator antigen, plasminogen activatoractivity, plasminogen activator inhibiter activity, plasmin activity and fibrinogen antigen Were measured before and after thrombolytic treatment with intravenous UK in 44 patients suffering from their first attack of acute myocardial infarction.

观察44例首次急性心肌梗塞患者的尿激酶静脉溶栓治疗前后血浆UK抗原、组织纤溶酶原激活抗原、纤溶酶原激活活性、纤溶酶原激活抑制活性、纤溶酶活性和纤维蛋白原抗原的变化。

The invention is directed a patterned contact sheet and to a method of bonding a cover wafer to an interposer wafer using the patterned contact sheet having a waffle-like pattern of a plurality of ridges and plurality of wells to form a cover/interposer combination or unit that can be bonded to a substrate having a MEMs device thereon, the cover wafer, interposer wafer and substrate together forming a protective packaging for the MEMS.

本发明涉及在制造过程中保护临界表面的形成有图案的接触片材以及一种使用形成有图案的接触片材将盖子片粘合到插入片上以形成盖子/插入组合或单元的方法,所述接触片材具有华夫饼干状图案的许多脊和许多井,所述盖子/插入组合或单元可粘合到具有MEM设备的基材上,所述盖子片、插入片和基材一起形成MEM用的保护性封装。

The deep water deposition means the deposition in the area where the water depth is larger than 30 meters, in which the major sediments are coarse sand and gravel. This kind of deposition is the relic deposition formed by the Pleistocene alluvial-lacustine deposition reworked under modern channel currents actions, and ususally the Holocene acummulation lack in the deep waters. The transitional zone deposition is the deposition between the low tidal level line and the 30 meters isobath, where the sediment types is changeable and the grain sizes are gradually coarsening from the low tidal line to the deep water area. This kind of deposition is developed by the deep water eroded sediments sorting under the transitional zone current action. The different types distributed along the isobath. The tidal flat deposition is the fine sediment deposition on the tidal flat which is flo sedimentation of the silty clay under the tidal flat dynamic action and ususally developed fluid mud on the lower tidal flat. The channel mouth deposition is the deposition in the channel mouth area. According to the sediment types, it includes sand deposition where there are coarse sediments supply and silt deposition where there are only fine sediments surpport.

峡道深水区沉积是指水深大于30米的峡道底部沉积,它以粗颗粒砂砾石沉积为特色,是现代峡道水动力对第四纪海退时发育的冲洪积和坡积进行改造形成的蚀余沉积,全新世沉积地层往往缺失;峡道过渡带沉积是指水深介于低潮位线至30米水深线之间发育的峡道边坡沉积,该带沉积以沉积类型多样为特色,自低潮位线至30米水深线,沉积粒级逐渐变粗,由粉砂质粘土逐渐递变为中粗砂,是峡道深水区沉积经多次分选沉积而发育的,基本平行于等深线分布的条带状沉积,在岸坡较陡岸段,过渡带下部可发育重力沉积;峡道潮滩沉积是指在低潮线与海岸线之间发育的细颗粒沉积,它是粘性细颗粒泥沙在潮滩动力作用下,发生动水絮凝沉积而形成的粉砂质粘土沉积,在低潮滩可发育浮泥;峡道口门区沉积则是在峡道汇入大海或海湾时,在峡道出口水流影响所及范围的沉积,根据峡道口门区供给泥沙的特性,峡道口门区沉积有粉砂质淤泥沉积和砂质沉积两种类型。

The comparisons of biochemistry between OP and OR rats The differences between OP and OR rats included not only body weight, but lipids metabolism and insulin sensitivity as well, characterized with insulin resistance, increasing in serum free fatty acids and ketone body, and hepatic TC and TG in OP rats. However, no significant differences were observed in serum TG, TC, LDL, HDL and fasting glucose between OP and OR rats.⑶Comparisons of metabolites in serum, urine and liver tissue between OP and OR rats①There were significant differences in amino acids concentration between OP and OR rats,especially in liver tissue, such as high concentrations in ketogenic and glucogenic amino acids in OP rats, suggesting differences in amino acids metabolism;② The different metabolites between OP and OR rats included increasing of various saturated fatty acids and decreasing of polyunsaturated fatty acids in OP rats;③The urinary metabolites analysis indicated that different structure or metabolism of gut microflora might exist between the two phenotypes, which probably influenced the regulation of body weight gain;④The end-products of catecholamines in urine and intermediates of krebs cycle in serum in OP rats were all up-regulated, suggesting that the activity of sympatheic nervous system and energy metabolism was higher in OP rats than OR rats.

胰岛素耐受实验和胰岛素敏感指数表明OP动的胰岛素敏感性较OR动下降,而OP大鼠血清中游离脂肪酸、酮体、肝脏总胆固醇和甘油三酯水平显著升高;但是,OP与OR大鼠血清中总胆固醇、甘油三酯、低密度脂蛋白、高密度脂蛋白和空腹血糖等的水平并无显著性差异;⑶肥胖易感与肥胖抵抗大鼠血清、尿液和肝脏组织提取中代谢的比较研究表明:①OP与OR大鼠的血清、尿液和肝组织提取中多种氨基酸的含量存在显著差异,并以肝组织中的差异氨基酸数量为最多,包括各种生酮和生糖氨基酸水平在OP组的升高,说明氨基酸代谢的差异是两种体重表型大鼠之间存在的重要差异特征之一;②OP与OR动肝脏和血清差异代谢中包含多种饱和长链脂肪酸的升高如十四烷酸、十六烷酸、硬脂酸等和多不饱和脂肪酸的下降如亚油酸和花生四烯酸,说明两种体重表型动的肝脏脂肪酸代谢存在明显差异;③长期高脂饮食喂养后,动的尿液代谢分析表明OP与OR动体内的肠道菌群结构存在差异,这些菌群上的差别可能在动体重增长的调节上产生影响;④与OR动相比,OP动尿液代谢中儿茶酚胺类递质的代谢终产如高香草酸、扁桃酸和4-羟基苯乙酸明显升高。

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段,应用H5亚型鉴定引则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段;在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法;检测方法的特异性试验结果表明,该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应;检测方法的敏感性试验结果表明,通用引扩增、H5亚型特异性引扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg;对20份临床样品检测中,有10份为AIV阳性,其中有5份为H5亚型病毒阳性。

Based on the complexity of the sources for the sediments in the Okinawa Trough, the sequential extraction procedure was applied to the hydrothermal influenced sediments in the core H9 recovered from the middle Okinawa Trough. Five fractions, including exchangeable, bound to carbonates, bound to iron and manganese oxides, bound to organic matter, and residual were acquired. Based on detailed analysis for major, minor and rare earth element compositions, the distributions of the elements in various speciations of the sediments were determined and the controlling mechanism was discussed. The variations of hydrothermal signatures in the core, partitioned from the terrigenous, volcanic and biogenic materials, have been demonstrated and the accumulation rates of some elements were evaluated.

本文针对冲绳海槽多种源沉积作用混杂的特征,采用顺序淋滤萃取方法对海槽中部受热液活动影响的H9岩芯沉积进行了分离,将沉积中分为可交换态、碳酸盐结合态、铁锰氧化结合态、有机结合态和残渣态五个部分,并对各个结合态中常量、微量和稀土元素组成进行了分析,确定了不同元素在不同结合态中分配的比例,分析了控制不同元素进入沉积的主要机制。

22 Of 22 pairs EST-SSR primers from Chinese cabbage/rape respectively had amplification products in soybean,wheat and maize,the rate of amplification was 81.8%,95.4%,100%,respectively;10 of 22 pairs EST-SSR primers showed polymorphism in monocotyledons wheat and maize,13 in dicotyledons oilseed rape and soybean,7 in both monocotyledonous and dicotyledonous crops.

(1)30对小麦EST-SSR引中,有29,28和26对引分别在玉米、油菜和大豆中有扩增产,可扩增率分别为96.7%,93.3%和86.7%;其中分别有12对和9对引在单子叶作小麦和玉米及双子叶作大豆和油菜中的扩增产显示多态性,有4对引在4种作中的扩增产均显示多态性。

In order to diagnose disease caused by muscovy duck parvovirus rapidly and accurately, and at same time, further study the taxonomic relationship between muscovy duck parivovirus and goose parvovirus, two pairs of PCR primers (LHMP1/LHMP2 and LHGP1/LHGP2) were designed respectively base on complete nucleotide sequences of Muscovy duck and Goose parvoviruses registered in the GenBank, The LHMP1 for the upper one and the LHMP2 for the lower one, corresponding to the specific nucleotides sequence 454-472 and 1173-1155 respectively in MDPV, and the LHGP1 for the the upper one and the LHGP2 for the lower one, corresponding to the specific nucleotides sequence 454-472 and 1173-1155 respectively in GPV.

为了能快速、准确地对番鸭细小病毒病进行诊断,同时为了能进一步弄清番鸭细小病毒与鹅细小病毒在分类学上是一种怎样的关系,比较GenBank中MDPV和GPV的基因序列,针对这两种病毒左阅读框编码非结构蛋白基因序列的相同区段,分别构建两对PCR引。第一对引LHMP1/LHMP2是根据MDPV的基因序列设计的,上游引针对MDPV基因序列的454~472;下游引针对基因序列的1173~1155,为19个碱基,扩增跨幅为719bp。

At pre-operation, post-operation 6 h, 3 d and 7 d detect the leave of plasm prethrombotic state molecular marker tissue factor, tissue factor pass inhibitory, thrombus precursor protein, plasminogen activator inhibitory-1(PAI-1), P-Selectin, Thrombin-Antithrombin complexes, D-dimer with ELISA, transmission immunity nephelometer. Results Malignant tumor patients, the levels of plasma TF, TpP, D-D in 6 h and 3 d of post-operation rose to peak value.

分别于术前、术后6 h3、d和7 d用ELISA、透射免疫比浊法检测其血浆血栓前状态分子标志D-二聚体(D-dimer, D-D)、血栓前体蛋白(thrombus precursor protein, TpP)、凝血酶抗凝血酶复合(thrombin-antithrombin complexes, TAT)、组织因子(tissue factor, TF)、P选择素(P-Selectin, P-S)、组织因子途径抑制(tissue factor pass inhibitory, TFPI)、纤溶酶原激活抑制1(plasminogen activator inhibitory-1, PAI-1)的水平。

Module history and module family data were saved in the form of ordered three-dimensional group, and then the extensibility and transformation of module-matter-element were studied.

该方法首先在元模型的基础上,结合模块自身特点,建立了模块元与模块族元,以有序三元组的形式规范化地保存了历史模块和模块族数据,并研究了模块元的可拓性及其变换。

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