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We study the expression levels of GLUT1 in 16 samples of esophageal cancer and their normal mucosa by reverse transcriptasepolymerase chain reaction and immunohistochemical staining, analyse the correlation between the expression level of GLUT1 mRNA and protein in the tumor cells. Then we study the expression levels of GLUT1 in 55 patients with esophageal cancer by immnohistochemical staining, analyse the relationship between GLUT1 expression and clinical features of the patients.

我们采用逆转录聚合酶链反应和免疫组化染色的方法,研究GLUT1 mRNA及蛋白在16例食管癌病人的肿瘤组织和相应正常食管粘膜的表达水平,同时分析食管癌组织中GLUT1在mRNA水平和蛋白水平表达的相关性;采用免疫组化染色的方法研究55例食管癌病人肿瘤标本的GLUT1表达水平,并分析其与肿瘤的临床特性及预后的关系。

Research for the Seismic Behavior of the Structural System with Limited Stiffness Outrigger and with Rigid Outrigger

在超高层建筑结构设计中,当采用常规的结构体系无法控制水平力作用下的结构水平位移时,往往会增设带水平伸臂桁架的加强层,限制外框柱的位移,使外框柱和核心筒变形协调,达到增加结构刚度,减小结构水平位移的目的。

An Economics Analysis of Overeducation and Policy OptionOvereducation is defined that the actual educational level of the workers is higher than the required educational level of workers.

当个体所受教育水平和工作技术水平不匹配,或者个体所受教育水平高于工作需求水平,就会发生过度教育的现象。

According to performance degree of the positive symptom as the below: compulsion, physical symptom, human sensibility, antagonism, depression, bigot, anxiety, terror, and psychoneurosis; the mental health state of the primary school teachers is better than the middle school teachers; the senior middle school teachers have the worst mental health state; the mental health level of male teachers are equal to the female teachers; the mental health level of the teachers in charge of classes are equal to the teachers not in charge of classes, there is not notable difference; the teachers mental health level in each age-phase almost the same; the factors of the primary and middle school teachers divorced are higher than the single teachers and the teachers married, there is not notable difference.

本研究采用SCL-90临床症状自评量表,对258名中小学教师进行测试,探讨中小学教师心理健康的状况,结果表明:中小学教师的心理健康水平低于全国普通人群,心理健康问题的检出率为15.9%,按其阳性症状的表现程度依次为:强迫、躯体化、人际敏感、敌对、抑郁、偏执、焦虑、恐怖、精神病性;小学教师的心理健康状况比中学教师好,高中教师的心理健康状况最差;男女教师心理健康水平在总体上基本相当;班主任和非班主任的心理健康水平基本相当,没有显著差异;各年龄阶段的教师心理健康水平基本相当;离婚的中小学教师各因子分略高于未婚和结过婚的教师,但差异不显著。

The investigation on radioactivity level s of radioactive source storehouses and surrounding environment were conducted in logging CO.

对大庆测井公司放射源库及周围环境的放射性水平进行了调查,调查内容分为环境空气气溶胶辐射水平、水环境辐射水平、环境地表γ辐射空气吸收剂量率及土壤环境辐射水平四个方面。

The investigation on radioactivity levels of radioactive source storehouses and surrounding environment were conducted in logging CO.

对大庆测井公司放射源库及周围环境的放射性水平进行了调查,调查内容分为环境空气气溶胶辐射水平、水环境辐射水平、环境地表γ辐射空气吸收剂量率及土壤环境辐射水平四个方面。

ELISA showed that antibody responses induced by propolis vaccine were faster than that of oil-emulsion vaccines and aluminum hydroxide vaccines;antibody responses induced by oil-emulsion vaccines were longer in duration than that of propolis vaccines and aluminum hydroxide vaccines;the maximum antibody responses induced by oil-emulsion vaccines and propolis vaccines were higher than that of aluminum hydroxide vaccines;Antibody levels were all significantly boosted by revaccination of these vaccines and in addition to this,oil-emulsion vaccines showed longer antibody duration.

这七种疫苗在抗体产生速度方面以蜂胶苗较快,而油剂苗和铝胶苗免疫后抗体上升速度较慢;在抗体水平的维持能力方面,油剂苗较蜂胶苗和铝胶苗更强;在产生最高抗体水平方面,油剂苗和蜂胶苗能力相当且较铝胶苗为高;在加强免疫的效果方面,这些疫苗二次免疫所产生的抗体水平均较一次免疫更高,蜂胶苗和铝胶苗在第93天时的二免抗体水平已降至同一免相当,但此时油剂苗有着较一免更高的二免抗体水平

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异;采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异;采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

Each medicine used in the study totally has obivious effect on lens sodium-pump. Either on the molecular and cellular level or on the histological and organic level , they all have their distinctive demonstration. 1.Ouabain、Digoxine、DMSO decrease the expression of αsubunit of sodium pump on mRNA level , the cell morphology and ultramicroscopic structure demonstrated destructive effect,it indicates the cause of cataract of the medicines.2.D-thyroxine、Amphotericin B、Vitamin E increase the expression of αsubunit of sodium pump on mRNA level ,from the demonstration of the cell morphology and ultramicroscopic structure ,it indicates the medicines have protective or anti-distructive effect on lens.3.The medicines have isoform-specific action on lens sodium-pump,it indicates exploitting isoform-specific medicine has a promising clinical application.4. Lens sodium-pumps are inhibited probably lead to cataract, so in clinical medication, especially to the cardiovascular doctor, while he is using the sodium-pump inhibitors, he should pay more attention to adjust the dosage and duration of the drug therapy. 5. To creat the animal model of cataract through inhibiting lens sodium-pump, whether it can be a kind of classical method or not, it is worthy of further research.

本研究所选用的药物对晶状体钠泵均有明显的影响作用,在分子水平、细胞水平、组织器官水平均有特异的表现:1、哇巴因、地高辛、二甲基亚砜三种药物可降低晶状体钠泵α亚单位在 mRNA水平的表达,在细胞形态和超微结构上表现为损伤效应,提示了其潜在的导致白内障的可能。2、D甲状腺素、两性霉素B、维生素 E三种药物可升高晶状体钠泵α亚单位在 mRNA水平的表达,从细胞形态和超微结构上的表现,提示了其对晶状体有保护作用或抗损伤效应。3、药物作用于晶状体钠泵具有重整异构特异性,提示了开发出特异的具有重整异构作用的药物是极有临床应用前景的。4晶状体钠泵抑制后可能导致白内障的发生,故临床用药,尤其是心内科医生在使用这些钠泵抑制剂时应注意调整用药剂量及药物疗程。5 通过抑制晶状体钠泵来制作白内障动物模型能否成为一种经典的制作方法,还有待于进一步研究。

The aims of disaster study and risk study are tent to unanimousness, both will offer the theoretical basis for sustainable development models. Regional safety construction based on disaster study is converting into regional risk management system based on risk study. So the authors consider that sustainable development model is the regional development model under a certain risk level, and the risk level relies on the regional safety construction level that integrates the regional economic level and social cultural system. Strengthening subject construction of disaster and risk study in China, national laboratory construction, and organization and practice of key items are imperative. And we should put the study emphasis on the vulnerability assessment system and models, risk assessment system and models, disaster and risk management mode of typical region, disaster and risk emergency control scenario, disaster and risk regions.

灾害与风险研究的目标正在趋向一致,都集中在为可持续发展模式提供科学依据;基于灾害研究的区域安全建设正在向基于风险研究的区域风险管理体系转变,由此认为可持续发展模式就是接受一定风险水平条件下的区域发展模式,一定风险水平依赖于由区域经济水平与社会文化体系相关联的区域安全建设水平;加强我国灾害与风险科学研究的学科建设、国家实验室建设、重大项目的组织与实施已势在必行;重点开展灾害脆弱性评价体系与评价模型、风险评价体系与风险评价模型、典型区域灾害与风险管理范式、灾害与风险应急控制预案、灾害与风险区划等方面的研究。

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推荐网络例句

It is also known as one of the most poisonous naturally occurring substances.

它也被称为一个最自然发生的有毒物质。

The greatest stress is found at the location on the cross section where V is the largest.

最大应力出现在横截面上V为最大的地方。

It is the most important three water problem which our country faces in the 21st century that flood and waterlog, drought and shortage of water, the deterioration of water environment.

洪涝灾害、干旱缺水、水环境恶化是二十一世纪我国面临的三大水问题。