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The P1 recombinant protein extracted and purified with B-PER GST Fusion Protein purification Kit was used as an antigen, and thallus component of full Mp was used as an antigen control to establish indirect ELISA experimental method to test and determine IgG antibody of serum samples from 40 normal blood donors and 51 clinical serum of partly certain and partly uncertain Mp infector.

以GST融合蛋白层析柱提取、纯化Mp P1重组蛋白做抗原,以全肺炎支原体菌体成分做抗原对照,建立间接ELISA实验方法,检测40份正常献血者血清标本和51份疑似MP感染临床血清标本的IgG抗体。

Cultured in MS medium with different concentration of calcium, explants of Luffa Cylindrica, which could not generate root in usual medium, generated roots with enhanced rates and amounts according to the concentration of supplemented calcium. Moreover, some explants show transformed root from either shoot or flower bud.

在正常Ca2+浓度下,仅激素处理不能生根的丝瓜外植体,处以不同Ca2+浓度处理时,随Ca2+浓度升高,外植体生根数明显加快并增多,且有少量植株叶腋处异化生根,在一定程度上改变了腋芽的发育方向,这说明Ca2+改变了卷须芽或花芽的发育方向,使其发育为根。

in order to probe into whether glycine supplementation plays an adjusting role in sports performance and immune functions, the authors divided 30 mice randomly into an exhaustive exercising group (e1), glycine supplemented exhaustive exercising group (e2) and a normal resting control group, with 10 mice in each group, supplemented glycine to mice in group e2 and physiological saline to mice in the other two groups, let mice in groups e1 and e2 do an exhaustive swimming exercise 4 weeks later, applied the trace ch50 hemolysis method to analyze supplement activity, applied the mtt method to perform a quantitative analysis on the lymphopoiesis ability of spleen, and thus revealed the following findings: compared with mice in group e1, the time taken by mice in group e2 to swim to an exhausted state is significantly longer (p.05); total supplement activity in blood serum of mice in group e1 is significantly lower than the same of mice in group s (p<0.01), and total supplement activity in blood serum of mice in group e2 is significantly higher than the same of mice in group e1 (p.01), but still significantly lower than the same of mice in group s (p.05); as observed from the test of the lymphopoiesis ability of spleen, the lymphopoiesis ability of spleen of mice in group e1 is significantly lower than the same of mice in group s, and the lymphopoiesis ability of spleen of mice in group e2 is significantly higher than the same of mice in group e1, but still significantly lower than the same of mice in group s.

四型肽精氨酸脱氨酶在类风湿性关节炎。。。以马来酸酐与氨水为原料热缩聚法合成。。。摘要:为探讨甘氨酸补充对运动能力及免疫功能是否具有调节作用,将30只小鼠随机分成力竭游泳运动组(e1)、甘氨酸补充后力竭游泳组(e2)和静息正常对照组,每组10只。在e2组补充甘氨酸,其余两组给予生理盐水,处理4周后,e1组和e2组均做力竭性游泳运动。采用微量ch50溶血法分析补体活性,采用mtt法定量分析脾脏淋巴细胞增殖能力。结果显示,与e1组比较,e2组游泳至力竭的时间显著延长(p<0.01)。e1组血清总补体活性显著低于s组(p<0.01),e2组显著高于e1组(p.01),但仍显著低于s组(p.05)。在脾脏淋巴细胞增殖试验中观察到,e1组显著低于s组,e2组显著高于e1组但仍显著低于s组。

The isolated nori cells or protoplasts can be cultivated into thalli for the totipotency of plant.

由于植物细胞的全能性,解离的单细胞或原生质体经培养能够正常发育成叶状体。

Results (1) Osteitis and destruction of end-plate showed long T1 and long T2.(2) Anterior and lateral abscess had long T1 and long T2 presenting even and structure-less signal.(3) The intervertebral discs showed normal or only degeneration at the early stage, and destroyed at later stage.(4) With Gd-DTPA enhancement, fibrotic granulation around abscess and destroyed area was well demonstrated.(5) Abscess in the bone and/or paraspinal abscess, destruction of end-plate were the important evidence for early diagnosis.

结果 (1)椎体骨炎及终板破坏呈长T1长T2信号,(2)椎旁或椎前冷脓肿呈长T1长T2均匀无结构信号,且上下跨越范围较大,(3)早期椎间盘信号正常或只出现退变,晚期则被破坏,(4)Gd-DTPA增强扫描可清楚显示冷脓肿周围纤维肉芽组织及椎管内侵犯,(5)骨内小脓肿和/或椎旁脓肿、椎体终板破坏是MRI诊断早期脊柱结核的重要依据。

Results: The proportional volume, numerical density of the granules in ovariotomy group were less than those in sham-ovariotomy and ovariotomy estradiol groups (P.001); the proportional volume and numerical density of ASG in myocardial cells almost came back to the normal level with small dose estradiol; along with the enhancement of estradiol dosage, the content of ASG in myocardial cells was more than those in sham-ovariotomy and small dose estradiol group (P.01), and showed a quantity-efficiency relation.

结果:切除大鼠双侧卵巢后,心房特殊颗粒的体密度和数密度与对照组及用雌二醇组比较明显减少(P<0.01),小剂量雌二醇可使大鼠心肌细胞心房特殊颗粒的体密度和数密度恢复到正常水平,随剂量增加,心肌细胞心房特殊颗粒的量也伴随增加,呈量效关系。

This is mainly ascribed to the tetragonal phase increase and the rhombohedra phase decrease.

这主要是由于四方相增多,三方相减少,从而使弛豫铁电体变为正常铁电体。

AIM: To isolate bioactive secondary metabolites from the fruits of Schisandra rebriflora Rehd. et. Wils and study their effects on HIV-1 infectivity. METHODS: Inhibition of syncytia formation, HIV-1 reverse transcriptase and protease, protection of HIV-1 infected cells, level of HIV-1 p24 antigen,blockage of fusion were detected. RESULTS: A lignan compound, named rubrifloralignan A, was first isolated as a natural product with modest inhibition effects on syncytium formation and HIV- 1 replication and could protect HIV-1 infected cells with weak inhibition effects on fusion and RT, but it showed no effects on the replication of HIV-1 chronic H9 and PR. CONCLUSION: Rubrifloralignan A is an efficient anti-HIV-1 product with effect on early stage of HIV-1 replication.

目的:从红花五味子果实中寻找活性代谢产物,研究其抗HIV—1活性和作用机制方法:通过合胞体抑制、HIV-1感染细胞保护、HIV—1p24抗原测定、融合阻断、逆转录酶和蛋白酶活性分析等实验,检测分离化合物的抗HIV—1活性并探讨其作用机制一结果:红花五味子甲素能够抑制病毒诱导的合胞体形成,保护病毒感染细胞,抑制病毒在细胞内的复制对HIV-1感染细胞与正常CD4^+细胞间的融合,逆转录酶活性有一定的抑制作用但是红花五味子甲素不抑制病毒在慢性感染细胞中的复制,也不抑制蛋白酶的活性、结论:首次从天然产物中分离得到红花五味子甲素,它对HIV—1病毒复制早期具有抑制作用

When normal platelets are stimulated by thrombogen, ADP or collagen, the GPⅡb/Ⅲa receptor on the surface of platelet combine with fibrinogen that induces platelet aggregation.

正常血小板在各种刺激下,如凝血酶原,ADP,胶原诱导下被激活,其表面糖蛋白Ⅱb/Ⅲa受体由配体-非结合状态变为配体-结合状态,导致血小板聚集,形成血小板血栓。

Working Principle Transmitter is composed of sensor and electronic element circuit, pressure of measured medium directly applies on sensor s ceramic diaphragm, force diaphragm to displace tinily in proportion to pressure of medium, under normal working condition, diaphragm displace no more than 0.025mm, electronic circuit detects this displacement, and convert it into standard industrial measuring signal i.e.

工作原理变送器由传感器与电子元件线路组成,被测介质的压力直接作用于传感器的陶瓷膜片上,使膜片产生与介质压力成正比的微小位移,正常工作状态下,膜片最大位移不大于0.025mm,电子线路检测这一位移量后,即把这一位移量转换成对应于这一压力的标准工业测量信号(4~20)mA。超压时膜片直接贴到坚固的陶瓷基体上,由于膜片与基体的间隙只有0.1mm,过载时膜片的最大位移只能是0.1mm,所以从结构上保证了膜片不会产生过大变形。

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