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Likewise, there was a significant result in the case of investigating whether luteal cells in early stage was affected by hypoxia similarly to those in middle stage, given big difference in the development of cell physiology. In this experiment, luteinized granulosa cells were used to mimic the early stage luteal cells. And luteinized granulosa cells were also cultured under the normoxic conditon and the hypoxic condition individually.

另一方面,由於中期黄体细胞发育已臻成熟,其细胞生理与黄体发育初期已然有所差异,因此本研究亦应用黄体化粒性细胞模拟发育初期之黄体细胞,并培养於正常氧或缺氧条件下,添加PKA讯息传递路径活化剂(oLH或8-bromo-cAMP)测定孕酮生成与相关蛋白质之表现差异。

OLH or 8-bromo-cAMP were added to luteinized granulosa cells. Results indicated that, protein expression of HIF-1a, HIF-1b and VEGF had a better performance in hypoxia than in normoxic condition, but phospho-PKA, StAR and P450scc protein expression had weaker expression in the former condition than in the later one. As a result of decreasing secretion of progesterone the decreasing of phospho-PKA was suspected to have a role in the reduction of StAR and P450scc in the condition of hypoxia.

结果发现,黄体化粒性细胞於缺氧条件下其HIF-1a、HIF-1b与VEGF蛋白质表现量皆较正常氧高;同时,StAR及P450scc蛋白质表现量则降低,但3b-HSD不受缺氧影响;此外,藉由特异性抗体检测PKA讯息传递路径发现,PKA讯息传递路经於缺氧条件下磷酸化PKA(phospho-protein kinase A)蛋白质表现量下降,可能经此路径的调控造成StAR及P450scc蛋白质表现量下降,导致黄体化粒性细胞孕酮分泌量减少。

Using normal roots from aseptic seedlings and hairy trots originated from Agrobacterium rhizogenes A4-mediated genetic transformation of L.

目的:探索光照和植物生长物质对宁夏枸杞无菌苗正常根和发状根离体生长的影响,建立离体根的最佳培养体系。

After operation, the density ofpositive nerve terminals and varicosities began to decrease on the third day, reached the lowest level on the 6th day and recoveredto normal level on the 12th day on operated side. But the number of BDNF neurons showed no obvious change.

术后3 d,术侧II板层阳性神经终末和膨体密度开始减少,6d减少至高峰,到第12 d时两者又均恢复到正常水平,而阳性神经元胞体的数量却无明显变化。

Methods Physical examination was undertaken for all patients with vulvae itching and vaginal discharge.

对601例拟诊为下生殖道感染的女性患者和306例正常体检的女性进行临床检查,阴道分泌物的pH值检测,假丝酵母菌、滴虫检测,胺试验和线索细胞检查,同时采用荧光定量PCR测定解脲脲原体、淋球菌、沙眼衣原体。

The methods introduced above are applied to the stability analysis of the Xiaowan high arch dam abutment and rock foundation. Thus, the deformation characteristics of the rock masses and the safety factors of the block combinations are obtained, which provide important data to the engineering design.

运用上述方法对小湾高拱坝的坝肩和坝基岩体进行了稳定分析,得到了正常工况下坝肩和坝基岩体的变形规律,以及各滑动岩块组合的强度储备安全系数,从而为工程设计提供了依据。

Methods The cervix mucus of 92 patients with spontaneous abortion and 45 patients with artificial abortion in normal gestation were detected for CT and UU, and corelative analysis was performed.

对92例自然流产患者、45例正常妊娠人工流产者的宫颈黏液做沙眼衣原体和解脲支原体检测,并进行相关分析。

Methods The cervix mucus of 92 patients with spontaneous abortion and 45 patients with artificial abortion in normal gestation were detected for CT and UU, and corelative analysis was performed.

对92例自然流产、45例正常妊娠人工流产者的宫颈黏液做沙眼衣原体和解脲支原体检测,并进行相关分析。

Results: 1 The analysis revealed that GAP-43 immunopositive neurons in rats were localized in all the layers of lateral geniculate nucleus and the layers II -VI of visual cortex area 17,and GAP-43 is stained by a ring and dot figure and brown-yellow colour in cytomembrane ; 2, In the critical period, gene expression concentration and number of GAP-43 immunopositive neurons, in both the lateral geniculate nucleus and the visual cortex area 17, received from the deprived eyes, are reduced.

结果:1、GAP-43在正常大鼠视觉系统表达主要见于外侧膝状体全层和视皮质17区Ⅱ—Ⅵ层神经元胞膜中,呈环状或点状棕黄色免疫阳性反应;2、在敏感期内剥夺眼对侧外侧膝状体和视皮质17区GAP-43表达的免疫阳性神经元染色变淡(P<0.05),且免疫阳性神经元数目减少(P<0.05)。

There was no abnormal behavior observed in meiotic chromosomes in carrot. It has simultaneous cytokinesis. There are nine bivalents (9II) at metaphase I, indicating it is a typical diploid species.

通过观察胡萝卜花粉母细胞的减数分裂过程,发现其行为正常,是同时型胞质分裂,中期Ⅰ有9个二价体(9Ⅱ),为典型的二倍体物种。

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