无菌的

The results showed that the bacteria B7 came into the logarithmic growth phase for 5 h culture. Both the fermentation liquid and its filtration could inhibit C. colhounii effectively, and the fermentation of dilution l0 times also showed significant differences compared with the control. The inhibiting effect of its fermentation media filtration was even more significant in the optimizing test, with the maximal inhibition rate of 100%. The best fermentation condition was 0.5% glucose, 0.1% ammonium muriate, without extra mineral, pH 5.4, temperature 28℃. Based on the morphological properties and 16S rRNA sequence, the bacterium B7 was identified as the Bacillus subtilis.

结果表明，该菌体在培养了5h后即进入对数生长期；B7细菌的发酵液原液和无菌滤液均能抑制病原菌的生长，其中发酵液原液的10^3稀释液的抑菌效果仍与对照之间存在显著的差异；发酵条件优化试验中发现该细菌的无菌滤液最大抑菌率达100%，最佳发酵条件为葡萄糖0.5%、氯化钱0.1%、不加无机盐、pH5.4，温度28℃；结合形态学特征和16S rRNA的序列分析，将该菌株鉴定为枯草芽孢杆菌。

Devices delivered in a sterile state must be designed, manufactured and packed in a non-reusable pack and/or according to appropriate procedures to ensure they are sterile when placed on the market and remain sterile, under the storage and transport conditions laid down, until the protective packaging is damaged or opened.

无菌器械的设计、生产和包装应采用一次性使用包装方式，并且在一定工作程序下保证器械上市时处于无菌状态，在贮藏、运输条件下只要包装不破损或开封，能够保持无菌状态。

Its principal use has been for surface sterilization of powders as a precaution against potential microbiological contamination of the sterile powder during aseptic handling.

乙撑氧也被用在无菌粉末的处理上，它的主要用途是对原粉表面灭菌，在无菌处理期间防止对无菌粉末可能造成的微生物污染。

One, suck phlegmy the preparation before works 1 should make sure negative pressure attracts plant above all each conduit joins correct, close together, unobstructed, assure significant negative pressure, general adult 10.64~15.96Kpa, the baby should be controlled in 7.98~10.64Kpa. 2 preparation rinse the asepsis physiological saline that smokes phlegmy pipe 2 bottles, 1 bottle wash pump technically with Yu Chong the suction inside tracheal spile is phlegmy canal , additional 1 bottle rinse those who had sucked mouth, nasal cavity technically to smoke phlegmy pipe . 3 equipment are very different the asepsis of model sucks phlegmy canal and asepsis glove. 4 because suck phlegmy itself,be a kind of stimulation to the patient, sober patient is not willing to suck normally phlegmy, and the family member of insensible patient also thinks to suck phlegmy special anguish.

一、吸痰前的预备工作1首先要保证负压吸引装置各管道连接正确、紧密、通畅，保证有效的负压，一般成人10.64~15.96Kpa，婴儿应控制在7.98~10.64Kpa.2预备2瓶冲洗吸痰管的无菌生理盐水，1瓶专门用于冲洗抽吸气管插管内的吸痰管，另1瓶专门冲洗吸过口、鼻腔的吸痰管。3备好不同型号的无菌吸痰管及无菌手套。4由于吸痰本身对病人是一种刺激，清醒的病人通常不愿意吸痰，而昏迷病人的家属也认为吸痰非凡痛苦。

Storage of drug products after release;(9) Control and laboratory operations;(10) Aseptic processing, which includes as appropriate: I Floors, walls, and ceilings of smooth, hard surfaces that are easily cleanable; II Temperature and humidity controls; III An air supply filtered through high-efficiency particulate air filters under positive pressure, regardless of whether flow is laminar or nonlaminar; IV A system for monitoring environmental conditions; V A system for cleaning and disinfecting the room and equipment to produce aseptic conditions; VI A system for maintaining any equipment used to control the aseptic conditions.

无菌操作及有关要求： I 地板，墙壁和天花板平滑，坚硬，表面易清洁； II 温度与湿度控制； III 空气经高效过滤器，在正压下过滤，层流或非层流均可； IV 环境检测系统； V 创造无菌环境，房间和设备的清洁，消毒系统； VI 控制无菌环境的设备维修系统。

Seeds of Pinus radiata were mixed with the finely divided sporophores in water.

由孢子包裹的种子发育成的苗木，形成许多须腹菌外生菌根在无菌土及缺乏外生菌根菌的非无菌土中。

For further understanding the effect of the strain on K-bearing silicate mineral, an experiment with KO2 strain cultured in nitrogen-containing and nitrogen-free medium was conducted. By putting mineral powders into the medium and measuring the concentration of capsular polysaccharides, we concluded that the strain can accelerate the weathering rate of silicate minerals. The results showed that the polysaccharides secreted by the strain in the growth process could help the bacterial to adhere to the mineral surface effectively, and created the bacterium-mineral complex, which formed a mircro-enviorment avail the release of potassium. Another result was that there was higher level of Carbonic Anhydrase activity, which revealed that some exo-protein or enzyme produced by the bacterial had postive impact on the process of releasing potassium ion. We carried out the bacterial fermentation for larger scale production of the bacterial secretion, which was used to sperate, and identify the small molecules related to the mineral-bacterial interaction. After the analysis, it was found that the strain can produce many kinds of small molecules, such as 2-Hydroxybenzoic acid, 4-Hydroxyphenylacetic acid, methyl-4-hydroxybenzoate, etc.

分别将菌株接入含有不同矿粉的培养基中培养，检测其在有氮、无氮培养基中荚膜多糖含量的变化，并在以钾长石和黑云母为矿源的情况下，比较研究了菌株在有氮、无氮培养基中对矿物的风化能力，通过一系列的实验，证实胶质芽孢杆菌在生长过程中所分泌的粘稠胞外聚糖可帮助细菌有效黏附在矿物表面，形成细菌-矿物复合体，并改变及维持该复合体内部的微环境，有助于该菌的解钾作用，而且细菌分泌的胞外蛋白质在该菌对含钾硅酸盐矿物的解钾作用过程中发挥重要作用；将胶质芽孢杆菌接入以钾长石和黑云母为矿源的有氮、无氮培养基中培养，检测到该菌碳酸酐酶活性的变化，并进行批量发酵后小分子酸性分泌产物的提取、分离、纯化与鉴定等方面的研究，结果表明，该菌可产生2-羟基苯甲酸，4-羟基苯乙酸，4-羟基苯甲酸甲酯等小分子物质。

Domestic companies to research and development of sterile, fresh packaging, revitalize national industry as its mission, has successfully developed more than 20 years of aseptic packaging 5-6 Composite paper, house-shaped packages, brick-shaped liquid, pincushion, housing shape, Triangle, ax-shaped drinks in aseptic packaging machine and ancillary support equipment, aluminum and Zhisu multi-storey complex in the printing paper, punching, indentation, composite, at all, the whole election, such as complete sets of equipment.

公司以研究开发国产化无菌、保鲜包装，振兴民族工业为己任，20多年来先后研制成功5—6层无菌包装复合纸、屋形包，液体砖形、枕形、屋形、三角形、斧形饮料无菌包装机以及相配套的辅助设备，和纸塑铝多层复合纸的印刷、冲孔、压痕、复合、分切、整选等成套设备。

Besides,the control of production techniques and the key techniques including sterile filtration and sterile bottle-filling etc.

主要叙述了纯生啤酒的特点，进一步阐述了纯生啤酒生产过程中如何控制生产工艺、无菌过滤和无菌灌装等重点工序，严格实现无菌化操作，从而实现生产出优质的纯生啤酒。

Ninety seven spores or sporocarps of VAMF were wet sieved from the rhizosphere soil of mycorrhized pteridophytes, from which 25 VAMF species which belonged to the genera of Acaulospora, Gigaspora, Glomus, Sclerocystis and Scutellospora respectively were identified, and the taxonomic characters of the 25 species were described. The Glomus fungi were the dominant VAMF in the rhizosphere soil of pteridophytes, they took up 44% in the identified 25 VAMF (11 species belong to Glomus); the fungi in Acaulospora and Sclerocystis in the next place, they took up 24% and 16% respectively in the identified VAMF; the fungi in the other genera took up 16%. In terms of the relationships of pteridophytes and the VAMF isolated from their rhizosphere soil, there was no specifity between the VAMF and their host.

从具有VA菌根的蕨类植物对应的根际土壤中用湿筛法筛取了97份VA菌根真菌的孢子或孢子果，从中鉴定出了分属于无醒囊霉属、巨孢囊霉属、球囊霉属、硬囊霉属和盾孢囊霉属的VA菌根真菌25种，并对这25种真菌的分类学特征进行了描述；球囊霉属真菌是蕨类植物根际土壤中的优势类群，已鉴定的25种真菌中，有11种属球囊霉属真菌，占44%，其次是无梗囊霉属和硬囊霉属真菌，分别占24%和16%，其他属的VA菌根真菌占16%，从分离鉴定的蕨类植物根际土壤中的VA菌根真菌与蕨类植物的关系看，VA菌根真菌与蕨类植物之间无明显的专一性。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。