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The main aberrations of the euchromosomes was chromosome gain, including trisome, tetrasome, and polysome. The gain rates of the four euchromo-some were 84.9%, 77.5%, 63.7% and 83.2%, and the frequencies of polysome for each euchromosome were 24.6%, 34.9%, 23.4% and 31.7%, respectively. Loss of chromosome Y was observed in 61.2% of male patients. The combination of the four chromosome probes 3, 8, 10 and 20 detected 74.5% of ESCC and the combination of 3, 8, 20 and Y detected 85.0%.

发现所测常染色体在食管癌组织中均存在较高的数目畸变率,主要表现为染色体增益,包括三体、四体及多体。4条常染色体3、8、10和20号染色体增益率分别为84.9%、77.5%、63.7%和83.2%,其中多体率各为24.6%、34.9%、23.4%和31.7%。Y染色体在61.2%的男性患者表现缺失。3、8、10和20号探针联合检测食管癌的阳性率为74.5%, 3、8、20和Y染色体探针联合检测男性食管癌的阳性率为85.0%。

Methods mRNA of placenta from normal pregnancy and PIH were extracted and labeled with flourescent Cy3 and Cy5 in reverse transcription,respectively.The Biodoor chip was hybridized with labeled cDNA.

方法从正常妊娠胎盘和PIH胎盘中抽提mRNA,反转录后,分别用Cy3、Cy5荧光标记,获得两组胎盘来源的cDNA探针。cDNA探针与含8 190条人cDNA点样的BioDoor基因芯片杂交,结果由激光共聚焦扫描仪扫描,并用软件进行统计分析。

The main intention of this thesis is to study some fundamental surface phenomena with chemical and/or physical interests by scanning probe microscope, and to exploit the instruments themselves into the research of surface chemical process, especially surface photochemical process.

本论文的研究目的在于使用扫描探针显微镜研究表面上的基本化学和物理现象,同时探索扫描探针显微镜在表面化学过程,尤其是表面光化学过程中的应用,所涉及到的主要研究内容和结论如下

In contrast, ITS2 could hybridize with DNA from both Paragonimu westermani and other species.

结果 COⅠ探针有较好的特异性和敏感性。ITS2探针缺乏特异性。

There six chapters in this dissertation: the overview on the study of probing for reactive oxygen species, spin-labelled fluorophore as probe for free radicals, Fe -EDTA chelate-induced aromatic hydroxylation of terephthalate as a new method for the evaluation of hydroxyl radical-scavenging ability, study of fluorimetric method for the determination of peroxynitrite, study of spiro-formed rhodamine B hydrazide as a novel fluorescent probe in analytical chemistry, the conclusion and prospect of the present research work.

本论文分六章,包括生物活性氧表征的研究进展、自旋标记荧光探针表征自由基的研究、利用Fe-EDTA引发对苯二甲酸芳环羟基化反应评价抗氧化剂清除羟基自由基能力的研究、荧光法表征过氧亚硝酸根的研究、螺环型罗丹明B酰肼化合物作为新型荧光探针的性质及应用的研究、结论与展望。

This paper consists of three parts:(1) application of Nano Indenter Ⅱ on new types of electronic encapsulation materials;(2) measurement of micro-scale plastic equation of state with Nano Indenter Ⅱ;(3) variation of micro-scale plastic equation of state after boiler tubes used over a long period of time.

论文有以下三部分内容:(1)纳米力学探针在发展新型电子封装材料中的应用;(2)用纳米力学探针压入法测定材料的微区塑性力学方程;(3)电厂锅炉管长期使用后微区塑性力学方程的变化。

The nanoscratch process which was revealed by the horizontal indenter is to discuss the effect of species of deposition time on the tribological character.

微结构利用奈米探测技术以水平的方式移动探针,会得到不同刮痕力量和压痕深度对探针所产生横向力和摩擦系数,再去观察讨论此氧化锌薄膜的特性影响。

Chapter 1: The principle and the development of intramolecular charge transfer fluorescence were described.

第一章:本章简要概述了分子内电荷转移荧光的原理及研究现状、蛋白质光谱探针的研究进展和DNA光谱探针的研究进展。

In order to improve the application of SPM lithography, SPM oxidation and its overlay lithography were studied.

为加强基于扫描探针显微镜的微细加工技术的应用,本文对扫描探针显微镜的阳极氧化加工及套刻对准进行研究。

This paper systematically introduces the basic principle, sample preparation, analytical procedure, age and error calculation in U-Th-Pb dating by electron microprobe and also gives the prospect of application in the structural analysis.

对电子探针U-Th-Pb定年方法的基本原理、样品的制备和分析、年龄计算方法和误差分析进行了较系统的介绍,并对电子探针定年在构造分析中的应用前景作了展望。

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