序列号

These sequences have been submitted to GenBank with Accession number EF554399, EF554400, EF554400, EF554401, respectively.

将这些序列提交GenBank分别获得登陆号为：EF554399，EF554400，EF554400，EF554401。

Then, two primers were designed from it, the full length cDNA of malate dehydrogenase gene in Chinese cabbage was gained through RT-PCR amplifying and sequencing and entered GenBank as accession number FJ208590, which is 1 209 bp length and coded 403 amino acids.

然后设计引物，经RT-PCR扩增、测序，获得了大白菜苹果酸脱氢酶基因的cDNA全长序列，并已将其登录到GenBank，登录号为：FJ208590，该cDNA全长1 209 bp，编码403个氨基酸。

A pair of specific primers was designed from the sequenceand was used to amplify the genomic DNAs of Yangmai 5,6VS/6AL line,substitution line of 6V and amphidiploid of Triticum durum-Haynaldiavillosa.

根据该序列设计了一对特异引物，分别以感病对照扬麦5号，抗病6VS/6AL易位系，6V代换系，硬粒小麦-簇毛麦双二倍体及簇毛麦的基因组DNA为模板进行特异性扩增。

Chromosome 22 is the first sequenced human autosome since Human Genome Project was launched.

22号染色体是人类基因组计划实施以来最先完成序列测定的常染色体。

The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result.

本发明提供了一种检测X、Y精子分离纯度的引物，该引物是针对牛Y染色体上性别决定基因Sry通过PCR错配技术设计而成，通过该引物可扩增片段大小为295bp，为了防止假阳性出现，本发明根据牛3号常染色体报道序列设计了一对内标引物C34，扩增片段大小为208bp，通过上述两对引物对单个牛精子进行双重PCR扩增，然后根据对检测结果的统计分析，对分离精子纯度做出最终评价。

The effect of tribology factors on bearing vibration is studied experimentally. AR model and WPT are used to processing the normal arid abnormal signals. The results are as follows:1. The vibration of ball bearings is caused by the tribology action of contact pairs of ball and ring races and has the essential of tribo-dynamics. Any factors affecting the tribology characteristics of contact pairs will affect vibration and noise of bearings consequently.2. Vibration characteristics of bearing keep almostly unchanged with the increasing of axial load expecting the increasing of nature frequencies. When radial load increasing with axial load unchanged, nature frequencies keep unchanged but some new vibration peaks appears in the spectrum.3. Vibration amplitudes are damped and nature frequency are enhanced with sufficient lubrication. Vibration and noise of bearings increase obviously and roar can happen and the contact surfaces scratch slightly under insufficient and unclean lubrication state. Bearing will be disabled in a few minutes without any lubrication.4. Bearing vibration is unstable under low speed. With the speed increasing, the vibration become stable and natural frequencies increase slightly but the amplitudes increase apparently.5. Bearing vibration can be excited only by some harmonics ofwaviness without lubrication and by all harmonics with lubrication based on the theoretical analysis.6. The nature frequencies of ball bearings decrease with the increasing sizes. When international clearance increasing, nature frequencies decrease in radial and increase in axial and angular and the amplitudes increase in radial and decrease in axial and angular. When the number of balls increasing, nature frequencies increase and amplitudes decrease. When the pitch diameter increasing, axial nature frequency increase and others nearly keep no change. When outer race groove curvature radius increasing, nature frequencies increase in radial and decrease in axial and angular and amplitudes keep no change in radial and increase in axial and angular. When inner race groove curvature radius increasing, nature frequencies decrease and amplitudes increase.7. The distortion in amplitude and frequency components of bearing vibration signals picked up by the present probe measurement method is founded and right conclusions cannot be achieved by the signals. When accelerometer is rigidly screwed with steel stud onto a flat outer surface of a ball bearing, vibration signal can be got without distortion and the reliability of research on bearing vibration is assured.8. AR model is suitable for large samples of bearing vibration signals, square root of length of samples can be used as the upper limitation of order determination and the FPE order selection criterion is effective. Many advantages of AR spectrum are founded over the classical based on FFT.9. Time-Frequency analysis is necessary for abnormal noise of ball bearings. WPT overcomes the principle shortcomings of STFT and proved to be a best tool to process the abnormal signals.

理论分析和试验研究表明： 1球轴承振动是钢球—滚道接触副中的各种摩擦学作用引起的，具有摩擦动力学的本质，任何对接触副的摩擦学特性有影响的因素都将对球轴承的振动和噪声特性产生影响； 2中心轴向载荷作用下，载荷增大使球轴承的固有振动频率升高，载荷越大同样的载荷增幅引起的频率升幅减小，足够大的中心轴向载荷作用下载荷的变化对球轴承振动的频率特性不会产生明显的影响； 3轴向和径向联合载荷作用下，径向载荷不大时球轴承振动的固有频率基本不变，但是可能引起变刚度激励的非线性振动，出现新的频峰，过大的径向载荷将使部分钢球脱离接触，使球轴承的振动和噪声呈现不稳定状态； 4润滑对球轴承的振动和噪声特性有重要的影响，良好的润滑对振动有明显的抑制作用，润滑不充分时，振动和噪声的水平会有明显增高，一定条件下还会激发接触副中的谐振，发出啸声，造成接触表面的伤害，无润滑干接触时，短时的运转就会损伤接触副表面，使振动和噪声迅速增大，并随时可能引发严重的磨损和卡滞失效；接触副润滑良好时，油膜的"刚化效应"使球轴承振动的固有频率有所提高，润滑剂中含有弥散性污浊时，振动的幅度总体升高，但弥散性污浊不会改变球轴承振动的频率特性；浙江大学博士学位论文：球轴承振动的研究 5低速时，球轴承振动的基本特征呈现不稳定状态，随着转速的提高，球轴承振动的频率特性趋于稳定，固有频率频峰升高； 6理论分析表明，干接触时钢球和滚道表面波纹度的某些谐波分量能激励球轴承的振动，振动的幅值与谐波幅值成正比，实际球轴承中钢球的分布不可能完全均匀，波纹度的激励作用会随时发生；润滑接触的分析表明，波纹度的任意谐波分量均能激励球轴承的振动； 7球轴承的几何和结构参数分析表明，球轴承尺寸越大，径向、轴向和角振动的固有频率越低；钢球中心圆直径增大，球轴承的径向和轴向振动的固有频率基本不变，角振动的固有频率有所上升，振动的幅频特性基本不变；径向游隙加大使球轴承的径向振动固有频率降低，轴向和角振动的固有频率有所升高，径向振动幅频特性的幅值升高，而轴向和角向降低；钢球的数量增多使球轴承振动的固有频率上升，幅频特性的幅值下降，径向振动的幅值下降最为明显；外圈沟曲率半径系数增大使球轴承径向振动的固有频率升高，轴向和角振动的固有频率降低，径向幅频特性基本不变，轴向和角向幅频特性幅值升高；内圈沟曲率半径系数增大使径向、轴向和角振动固有频率均下降，振动幅频特性的幅值均有升高； 8试验对比表明，传感器采用探针式安装时，由于探针接触副接触特性的影响，钡(量得到的球轴承振动信号有失真，采用专门设计和制作的试验球轴承，以固定式安装加速度计，首次测量得到了球轴承振动的真实信号，通过对振动信号的分析，验证了球轴承振动的理论模型； 9)基于时间序列分析的AR模型适用于大样本球轴承振动信号的分析，以样本长度的均方根值作定阶上界，FPE做判阶准则，给出的AR谱光滑，频率分辨率高，是球轴承振动分析的简便而可靠的手段； 10以时频域分析的小波包变换分析球轴承异音信号能够比较好地定位和聚焦异音发生的时间，时间间隔，频率范围，同样是球轴承振动分析的可靠的手段，可用作球轴承故障诊断技术。

Fluorescence in situ hybridization with 16S rRNA-targeted oligonucleotide probes was applied for analyzing the community of iron bacteria in wastewater from the Shengli Oil Field. Four 16S rRNA probes were designed, which were highly specific to iron bacteria Leptothrix, Sphaerotilus, Leptospirillum ferrooxidans, Leptospirillum ferriphilum and Acidithiobacillus spp.

根据油田外排水中常见铁细菌的16SrRNA特异性保守序列，设计和合成了4种荧光探针，利用荧光原位杂交方法，分别利用它们对胜利油田孤岛采油厂、胜利采油厂1号和2号综合处理站外排水中存在的铁细菌的种类和数量进行检测，分析了各外排水中铁细菌的群落结构。

Results Mobiligy shifts of SSCP in exon 5 of presenilin-1 was detected in 4 cases with Alzheimer,s disease. Two missense mutation were found in the patients by DNA sequence analyse, one mutation was Leu 130 Met and the other was Vall 57 Leu.Another 11 patients showed one single strend shifted rapidly. But none mobility shifts of SSCP were found in patients with vascular dementia and normal controls. Conclusions It is shown that mutations in exon 5 of presenilin-1 also exist in the patients with sporadic Alzhemer?

结果 发现4名SAD患者的PCR产物SSCP分析发生泳动异常，DNA序列分析发现：这4名SAD患者的130号密码子发生了CTG→ATG错义突变(388位点发生C→A突变)，使编码的氨基酸由亮氨酸变为蛋氨酸(Leu 130 Met)；157号密码子发生了GTG→CTG错义突变(469位点发生G→C突变)，使编码的氨基酸由缬氨酸变为亮氨酸(Val 157 Leu)，另有11名患者SSCP表现为一条单链增快，其性质待定。

Based on the screening of chlorpyrifos biodegrading fungal strain, Cladosporium cladosporioides (the ID of fungi preservation CCTCCM207111. Accession number of ITS sequence analysis Genebank EF405864), extracellular enzymes were extracted. Applying the composite rotatable design, a model of the optimal mixture protect agents was established. After the optimal composition was validated, the enzyme preparation storage experiment was tested to determine the stability of biodegradation by high performance liquid chromatography analysis.

在自主筛选毒死蜱高效降解真菌Cladosporium cladosporioides（菌种保藏号CCTCCM207111，ITS序列分析GeneBank登陆号EF405864）的基础上，发酵提取其胞外酶，采用&通用旋转回归法&筛选了酶活性保护剂的最佳配方，并进行验证；利用所得配方进行制剂贮藏实验，利用HPLC检验其降解性能稳定性。

The research aimed to seek the difference on rDNA-ITS sequence between Focr 1 and Focr 4 biological races of banana fusarium wilt and provide basis for rapid detection of banana difference wilt race.

寻找香蕉枯萎镰刀菌1号生理小种和4号生理小种(Focr 4)在rDNA-ITS区段序列上的差异，为香蕉枯萎镰刀菌小种的快速检测提供依据。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。