序列号

Segments of Adh gene in azuki bean (variety KS 5), covering an interval between partial intron 3 and intron 9, were amplified and sequenced with two pairs of primers. The result has proven the viability of using conserved sequences in a completely sequenced gene of one species to clone same gene in another species. In the comparison between Adh genes of azuki bean and pea, high sequence similarity (about 80%) in exon and long length difference (up to 116 bp) in intron were found. Besides, the edge regions of Adh introns with GT/AG counterparts and more conserved sequences than central regions was observed in both species.

利用两组引子对完成红豆(高雄5号)Adh基因之部分解序，其范围包括部分intron 3至intron 9之间的区域，也印证利用基因之已知保守序列可选殖另一物种之相同基因，红豆与豌豆之Adh基因序列比对结果显示两者之exon均较intron有较高之GC百分比(分别为41.0 ~ 48.7%及23.3 ~ 35.2%)，且两者在exon区域之DNA序列相似程度约80 %，而在intron区域中则有明显的序列差异存在(长度差异最高可达116 bp)，但是intron外侧序列均具有GT/AG组成且相对较中央序列具有保守性。

Among the 3 novel sequences, C24 sequence and C57 sequence were expressed both in cataractous group and in normal group; C24 sequence was expressed higher in cataractous group than that in the normal group, but C57 sequence had no significant difference in normal and cataractous group; C40 sequence was expressed in the cataractous lens epithelium but the expression in normal lens epithelium was not seen.

三个新序列中，C24号和C57号序列在年龄相关性白内障和正常晶状体上皮细胞中均有表达，C24号序列的表达增高；C57号序列在正常组和白内障组中的表达无差异。C40号序列在年龄相关性白内障晶状体上皮细胞中有表达，但其在正常晶状体上皮细胞中未见表达。

Gene sequence was highly homologic with a gene encoding aβ- subunit of ATP synthetase and an EST originating from a cDNA in relation to water deficit stress, which both were closely associated with the protein functioning in stress resistance and ion transportation. The results suggested that No. 1 gene sequence was involved in stress resistance of apple rootstock. No. 2 gene sequence had high homology with a gene encoding a serine/ threonine kinase protein, indicating perhaps it was a new gene encoding the serine/ threonine kinase protein in apple rootstock. The gene sequences of A1 and A2 were highly homologic with a gene encoding aβsubunit of ATP synthetase.

对得到的差异片断进行回收、测序，通过BLAST和其它植物比对，发现Ⅰ号序列与一条编码ATP合酶β亚基基因和一条水分亏缺胁迫cDNA克隆基因EST的同源性非常高，该序列编码涉及到了抗性表达、离子转运基因，可能参与了植物抗性表达，说明获得的基因序列应是与苹果砧木抗性相关的基因；Ⅱ序列与一条serine/threonine kinase蛋白激酶的蛋白序列同源性比较高，核酸比对较低，可能是具有丝氨酸/苏氨酸蛋白激酶功能的新基因。A1、A2序列与一条编码ATP合酶β亚基基因的同源性较高。

RESULT ①Two different ITS2 sequences (GenBank accession number: AF416783, AF416784) and D3 sequences (AF416782, AF415594), named as An. minimus A and An. minimus C, were detected in this study. They differed by 5. 8％ in the ITS2 region with 22 fixed nucleotide substitutions and indels, and by 1. 5％ in D3 gene with 5 site mutations. There exists individual variation in the COII gene, but this variation is less than that among other members of Myzomyia Series such as An.

结果 ①分别发现两种不同的ITS2序列（GeneBank登录号：AF416783，AF416784）和D3序列（GeneBank登录号：AF416782，AF425594），与微小按蚊A和C同源。A、C两者ITS2序列22个固定位点存在碱基置换和插入／缺失，序列差异为4.8％；D3基因存在5个固定点突变，突变率1.5％。mtDNA-COII基因存在个体差异，但远小于同系其它成员如乌头按蚊、杰普尔按蚊间的变异。

In contrast,the expression levels of Lb est2 in nymphal stages are lower than that in adult stage.2 Gene cloning of AChE and mRNA expression level in L.entomophila2.1 Gene cloning and sequence analysis of AChE in L.entomophilaTwo full length cDNA encoding AChE were cloned from L.entomophila by the methods of RT-PCR and RACE,named Le ace1(GenBank Accession No.:EU854149) and Le ace2GenBank Accession No.

CarE基因在不同发育阶段mRNA表达量的分析结果表明，Lb est1的表达量随着该虫生长发育的进行逐渐降低，至成虫期时表达水平最低；相反，L6 est2在若虫期表达水平较低，而在成虫期表达水平最高。2嗜虫书虱乙酰胆碱酯酶基因克隆及其mRNA表达水平研究2.1嗜虫书虱乙酰胆碱酯酶基因克隆及序列分析利用RT-PCR和RACE技术成功克隆获得嗜虫书虱2个AChE基在的全长序列，分别命名为Le ace1(GenBank登录号：EU854149)和Le ace2(GenBank登录号：EU854150)。

All of the 58 restriction endonucleases were used to investigate the mtDNA diversity. Four restriction endonucleases, DraⅠ、SnaBⅠ、SspⅠ and Vsp Ⅰ, were polymorphic. Based on presence or absence of the the 215〓 SspⅠ site, all of the 13 mtDNA sequences were divided into two groups.

利用DNAClub软件对AT富集区和tRNA〓基因的DNA序列进行了模拟酶切，所有的58种内切酶中有10种内切酶在13个材料中有酶切位点，仅4种酶DraⅠ、SnaBⅠ、SspⅠ和VspⅠ表现出多态性，且主要位点差异在于SspⅠ酶切位点的有无（序列的第215位的C/T）。12个品种中有4个品种（豫早1号、豫早2号、鲁红、33）没有此酶切位点，另外8个品种有此酶切位点，野柞蚕也没有此酶切位点。

Three primer pairs located in psbA~trnK, psbB~psbH and trnR-ACG~trnN-GUU regions were applied in phylogenetic analysis according to Maximum parsimony and Neighbor-joining methods. The consistent results between the phylogenetic analysis and taxonomy indicated the application potential of the three primer pairs in phylogenetic analysis and species identification. However, due to no sequence variation observed in amplified regions between two vegetable soybean varieties (KS5 and KS8), two cowpea subspecies (Vigna unguiculata ssp. cylindrica and Vigna unguiculata ssp. sesquipedalis), and among three rice varieties (TNG67, TK9, and TCS10), the three primer pairs are not effective in identifying variation within species.

选择其中三组引子对(基因体位置为psbA~trnK、psbB~psbH及trnR-ACG~trnN-GUU)增幅片段之序列进行亲缘分析，以最大简约法及邻接法之分群结果皆与目前植物分类相符，故三组引子对在物种间亲缘关系分析及物种鉴定上都有不错的效果，位於psbA~trnK及psbB~psbH之引子对效果尤佳，但是三组引子对在物种内变异的辨识效果不甚理想，在两个毛豆品种间(高雄5号与高雄8号)、两个豇豆亚种间及三个水稻品种间(台农67号、台稉9号与台中秈10号)的增幅片段没有明显差异存在。

It is very helpful to have the Quincy air compressor model and serial numbers available in order to determine the correct original equipment Quincy filters needed for the application.

这是非常有帮助的昆西空气压缩机型号和序列号可用以确定正确的原始设备昆西过滤器的应用程序所需的。

Assists in PC Troubleshootingand diagnostics. The best value professional burn-in tool on the market! Avoid delivering D.O.A. hardware to your customers. Dramatically reduce your burn in times with multithreaded simultaneous testing of components.

在这一版中主要作出了以下变化：支持同时测试多块磁盘、支持同时测试多个网络地址、支持同时测试多的串口、支持WindowsME、可以侦测出CPU Pentium4、Duron、支持侦测CPU的序列号功能、加入了CD测试功能。

You Can't use the SN on this Computer.

您不能再这台计算机上使用该序列号。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。