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For cloning antifreeze protein gene from a desert darkling beetle Microdera punctipenis dzunarica in Xinjiang, the primers were designed according to the core sequence of AFP gene deposited in GenBank, and the cDNA fragment about 294 bp named as MpAFP5 was amplified with the RT-PCR and 3'-RACE technique. Sequence analysis revealed that the cloned cDNA fragment named as MpAFP5 coded the mature peptide of AFP.

根据GenBank中已发表的昆虫抗冻蛋白基因的保守序列设计引物，利用RT-PCR技术结合3'-RACE扩增的方法，从新疆荒漠昆虫准噶尔小胸鳖甲Microdera punctipenis dzunarica中获得了长约294 bp不含信号肽的抗冻蛋白cDNA片段，命名为MpAFP5，其全长序列为363 bp(GenBank注册号为：AY821792)。

At least, we find out the score and monitoring indicators for each other by their check point. We also find out the diverseness on peak, trough, state and cycle by the graph of time series.

我们利用这些检查值在最后各找出了一组景气分数和灯号的结果，并利用时间序列走势图来观察两者在高峰、低峰、景气状况和景气周期上的差异性。

It is also worth noting that an earlier eclipse of the same Saros (141) in 1955 yielded an annular eclipse in Hong Kong, though not the whole territory could have a glimpse of it.

值得留意的是於 1955 年香港部分地区曾可见日环食，该日食同属沙罗序列 141 号。

Some characteristics of the novel gene YH1 was explored by bioinformatics. The YH1 gene was mapped on the chromosome 20 with the size of 7. 5 kb or so by blasting to genome database and this fragment size for YH1 gene coincided with the result of Souther blot. There were nine exon and eight intron.

利用生物信息学方法，在基因组数据库中比较，发现YH1基因存在于一个定位在20号染色体上7.293kb的DNA序列中，这与Southern blot结果相符。9个外显子被8个内含子隔开。

A total of 42 positive differential fragments were obtained,of which 5 fragments had no homolog in the NCBI and EMBL.

在获得的42个阳性阶段特异性表达的基因中，有5个在NCBI和EMBL数据库中没有同源序列，登录EMBL，申请了登录号。

Methods: The coding region of superoxide dismutase was amplified using PCR method from the E.co1i genome. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the cloned coding region of Mn-SOD was inserted into the expression vector PET-28a to form the recombinant plasmid PET-28a-Mn-SOD and was then transformed into E.coli BL21 for expression.

用PCR方法从大肠杆菌2号基因组中扩增Mn-SOD基因编码区，克隆到pUCm-T Vector，测定核苷酸序列；再将基因编码区克隆到原核表达载体PET-28a，构建含Mn-SOD基因的重组表达质粒，转化到大肠杆菌BL21中进行IPTG诱导表达。

Coordinate sequence method is made up of the instruct information of polygon boundary x, y coordinate. It is the easiest polygon vector encoding method. The file structure of this method is easy, but because of the polygon boundary has stored two times, it will produce data redundancy, moreover, short of neighbor threshold information; Tree index encoding method make all the boundary points digitizing, store the coordinate sequence, connect the point index to boundary line, and then connect the line index to other polygons, so as to the tree index structure has came into being. It eliminates neighbor polygons boundary data redundancy problems; Topology structure encoding method is a method which by setting up a complete topology relationship structure, resolve the neighbor threshold and island information process problem. However, it makes arithmetic more complicated and makes database bigger.

坐标序列法是由多边形边界的x，y坐标对集合及说明信息组成，是最简单的一种多边形矢量编码法，文件结构简单，但多边形边界被存储两次产生数据冗余，而且缺少邻域信息；树状索引编码法是将所有边界点进行数字化，顺序存储坐标对，由点索引与边界线号相联系，以线索引与各多边形相联系，形成树状索引结构，消除了相邻多边形边界数据冗余问题；拓扑结构编码法是通过建立一个完整的拓扑关系结构，彻底解决邻域和岛状信息处理问题的方法，但增加了算法的复杂性和数据库的大小。

In the present study, full-length rDNA sequences from the domestic and wild type of Chinese oak silkworm, Antheraea pernyi (GenBank No. GU073314 and GU073315, respectively) were obtained and comparatively analyzed on their genetic variation.

测定了放养型和野生型柞蚕的rDNA ITS-2全长序列（GenBank登录号：GU073314、GU073315），并分析了二者之间的遗传差异。

Fragments from the 239th to the 944th nucleotide of the coding regions of all actin mRNAs were amplified with two actin specific primers. Fragments from the 239th nucleotide of the coding region to the end of actin mRNAs were also amplified The electrophoresis results were compared between the macropterous and brachypterous brown planthoppers.

利用两条肌动蛋白特异性引物，扩增出了肌动蛋白基因mRNA的编码区片段，另外，将肌动蛋白特异性引物作为上游引物，将与3'锚定反转录引物部分序列相同的引物作为下游引物，扩增出了从肌动蛋白基因mRNA的编码区第239号碱基到Poly尾的这段cDNA，并在翅型之间作了比较。

The 12940 bp nucleotide sequence of the mitochondrial genome of a brachyura dacapod, Eriocheir japonica sinesis, was determined. The arrangement of 27 genes of the mitochondrial DNA differs from that of the mitochondrial DNA in all other arthropods studied.

中文题名中华绒螯蟹线粒体基因组与16S rDNA遗传标记研究副题名外文题名 The mitochondrial genome of the mitten crab，Eriocheir japonica sinensis(Crustacea：Decapoda：Brachyura) and genetic marker from mitochondrial 16S rDNA 论文作者孙红英导师周开亚教授学科专业动物学研究领域\研究方向学位级别博士学位授予单位南京师范大学学位授予日期2002 论文页码总数80页关键词蟹中华绒螯蟹线粒体基因组遗传标记馆藏号BSLW /2003 /Q959 /30 测定了短尾类十足目动物中华绒螯蟹的线粒体基因组12940 bp的核苷酸序列。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。