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Objective To explore the roles ofα-amanitin in the replication ofhepatitis B virus in HepG2 2.2.15 cell line Methods MTT assay were used to observe the cytotoxicity ofα-amanitin to the HepG 2 2.2.15 cell line.

中文摘要:目的:基于α-鹅膏毒肽的作用机制和乙肝病毒复制转录特点,初步探讨α-鹅膏毒肽对HepG2.2.15细胞的毒性及其对乙型肝炎病毒复制的影响。

These provisions changed the remedies for the circumvention of copy prevention systems (also called "technical protection measures") and required that all analog video recorders have support for a specific form of copy prevention commonly known as Macrovision built in, effectively giving Macrovision a monopoly on the analog video recording copy prevention market.

这些规定,改变了补救措施,为规避防止复制系统(也称为"技术保护措施"),并要求全部的牵拟录像机有支持某一特定形式的复制预防俗称Macrovision公司兴建的,有效的给予Macrovision的垄断地位模拟视频记录副本预防市场。

We reproduced the Munsell chips whose hues are 5R, 5YR, 5Y, 5GY,5G, 5BG, 5B, 5PB, 5P,5PR and values are 3, 5, 7, and chromas are 2, 4, 6, 10 using the sublimate printer. We then calculated the color differences ΔEab between the reproduced chips and the standard chips.

而Munsell色票为目前工业设计及平面设计等各行各业最常用的色票,因此我们以Munsell 色票中的十个主色相、彩度C=2,4,6,10、明度V=3,5,7的色票为本研究的标准色样,用热升华式印表机进行色彩复制,再计算出复制色票与标准色票的CIELab色差,以探讨热升华印表机复制Munsell色票的可行性。

Duplicate it then rescale it to about 0.87 and move it to around 2.254 of z axis and then duplicate again, rescale it to about 0.754 and move it to around 2.178 of z axis.

复制那条曲线,并将新复制出来的曲线在 xyz 轴上缩小到 0.87,在 z 轴方向上移动到 2.254 ;再次复制一条新的曲线,并将其在 xyz 轴上缩小到 0.754,在 z 轴方向上移动到 2.178 。

The 329 bp terminal inverted repeat sequence can't form the conserved fold-back secondary structure as that of many Streptomyces linear replicons. Lacking of typical Streptomyces tap/tpg locus for te-lomere replication, pPR2.3c encodes a protein with two domains resembling the telomere associated protein of Strepto-myces and helicase of Haemophilus respectively. No typical Streptomyces iteron-rep locus for replication from the cen-trally located origin, two DNA fragments containing almost all pPR2 were cloned and introduced by transformation into S.

其端粒末端反向重复序列的长度为329 bp,不能像多数链霉菌的线型质粒那样能形成保守的&折返&的二级结构。pPR2虽然没有参与链霉菌端粒复制的保守的tap/tpg基因,但是pPR2.3c基因编码了一个双结构域蛋白,分别同链霉菌的端粒复制相关蛋白Tap和嗜血杆菌的解旋酶具有相似性。pPR2缺少典型的链霉菌重复序列-复制基因区段,将几乎覆盖全长pPR2的两段DNA进行克隆后,不能转化变铅青链霉菌。

Objective To construct expression vector of HBV infectious replicon and establish hepatitis B virus replication model in vitro, and observe the dynamic changes of viral replication and its sensitivity to antiviral durg.

目的 构建乙型肝炎病毒感染性复制子表达载体,建立HBV体外复制细胞模型,分析其复制特性及对抗病毒药物的敏感性。

It was demonstrated that the dependence or independence of recA gene is not related to the direction of replication of the plasmids or phages in the free state.

实验结果说明,依赖与否和它们在游离状态中复制方向无关,从而否定了recA基因的作用是把质粒或噬菌体的游离状态的单向复制发动机构改变为整合状态的双向复制发动机构这一假设。

Those viruses, which can selectively replicate in and kill tumor cells, were called conditional replicative viruses or oncolytic viruses.

这种能选择性地在肿瘤细胞中复制,并进一步杀灭肿块,被称为条件性复制病毒或选择性复制病毒(conditional replicative viruses),或溶瘤病毒。

The replicative recombinant adenovirus is more effective than the replication defective adenovirus used generally at present in replicative ability, oncolytic ability and the expression of exogenous gene in the tumor cell.

构建的复制型重组腺病毒在肿瘤细胞内的复制能力、溶瘤特性和外源基因的表达明显优于目前广泛应用的复制缺陷型重组腺病毒。

Despite an attempt to prevent this by Aegir by attacking the Replicator ship as it emerged from hyperspace, before it could raise its shields, many Replicators survived and landed on the planet, among them the human-form Replicator known as "Fifth".

asgard Aegir 试图攻击刚脱离超空间且无法升起防护罩的复制船并阻止它入侵,可惜很多复制存活并降落在这个星球上,其中包含以人的形式被称为&第五&的复制者。

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If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了,请告诉我,我会帮助你摆脱困境,真的,请联系我啦!

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放,就是一个这样的例子。

Well, Jerry would rattle off all the details of that movie.

那么,杰瑞会急促背诵那部电影所有细节。