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METHODS AND RESULTS: Using both primary peritoneal macrophages and studies in advanced atheromata in vivo, we introduce signal transducer and activator of transcription-1 (STAT1) as a critical and necessary component of endoplasmic reticulum stress/type A scavenger receptor-induced macrophage apoptosis.

方法和结果:通过使用原代腹膜巨噬细胞研究在体脂质斑块,我们引入信号转导子和转录激活子-1(STAT1),作为内质网应激/A型清道夫受体诱导的巨噬细胞凋亡中关键和必要组分。

Fragments from the 239th to the 944th nucleotide of the coding regions of all actin mRNAs were amplified with two actin specific primers. Fragments from the 239th nucleotide of the coding region to the end of actin mRNAs were also amplified The electrophoresis results were compared between the macropterous and brachypterous brown planthoppers.

利用两条肌动蛋白特异性引物,扩增出了肌动蛋白基因mRNA的编码区片段,另外,将肌动蛋白特异性引物作为上游引物,将与3'锚定反转录引物部分序列相同的引物作为下游引物,扩增出了从肌动蛋白基因mRNA的编码区第239号碱基到Poly尾的这段cDNA,并在翅型之间作了比较。

They were also observed in sera from patients with cryptogenic cirrhosis or chronic hepatitis C.

这些转录体在某些隐源性肝硬变和丙型肝炎患者血清中也存在。

The inhibitor for DNA methyltransferase, 5-azacytidine (5-azaC), can demethylate the methylated DNA and restore transcription of genes. High dose homocystine can mediat DNA methylation and suppress gene expression.

应用甲基转移酶抑制剂5-氮杂胞苷(5-azacytidine, 5-azaC)可以使甲基化的启动子去甲基化,启动基因重新转录;高浓度同型半胱氨酸(homocystine, Hcy)可以介导基因甲基化而抑制其表达。

Department of Pulmonary Disease, Qilu Hospital of Shandong University, Jinan 250012, ChinaObjectiveTo study the relationship between transcription factor T-bet/GATA -3 and expression of α-smooth muscle actin and type Ⅰ collagen in asthma.

目的观察大鼠肺转录因子T细胞表达的T盒/GATA连接蛋白-3 (GATA3)对肺支气管α-平滑肌动蛋白和Ⅰ型胶原的调解作用,探讨其比率与哮喘早期气道重塑的关系。

Using cytochalasin D to prevent global actin from forming filamentous structures, the production of Heliothis armigera nuclear polyhedrosis virus in Hz-AM1 cells grown in the medium containing 0.5μg/ml CD was completely inhibited while its production was reduced to 10〓 in the presence of 0.1μg/ml CD by plaque assay. However, Western blotting revealed that the actin quantity in infected host cells treated or untreated with CD was almost identical. Additionally, CD had no effect on the DNA synthesis of HaNPV.

Western blot表明细胞松弛素D并不影响受染细胞中肌动蛋白的含量,Dot blot表明病毒DNA的合成也没有受到影响,但电子显微镜超薄切片发现在0.5ug/mL细胞松弛素D中形成的病毒粒子形态与正常的有明显区别,认为细胞松弛素D抑制棉铃虫核型多角体病毒的增殖是因为抑制病毒的DNA和蛋白组装成完整有感染性的病毒粒子,即丝状肌动蛋白对病毒粒子的脱壳和DNA的复制、转录没有影响,但对病毒粒子的组装是必须的。

CRM genome screeners screen sequences or complete genomes for CRMs as homotypic or heterotypic clusters of binding sites for any combination of transcription factors.

CRM基因组筛选器作为任何组合的转录因子的同型的或异型的结合位点类为CRMs筛选序列或全部基因组。

But it is also difficult to identify a mutant related to Tos17 because there are multiple copies of Tos17 in a mutant and only about 10 percent of mutants are from Tos17 insertion. Theoretically, Ac/DS two-element system is regarded as a best approach for rice insertional mutagenesis.

Tos17是目前应用最为成功的反转录转座子,但多拷贝的插入使突变体的表型鉴定和分子鉴定较为困难,因为只有10%左右的突变性状是由Tos 17插入引起的。

The recombinant plasmids constructed successfully were then transfected into HepG2 cells and expressions of interceptive AIF were detected by RT-PCR and Western blot.

成功检测到截断型在转录与翻译水平上的表达。

The main component include promoter sequence (5' flank control rigon of goat P - lacto globulin gene of goat),foreign gene,Marker gene and genome of defective retrovirus.

其中主要构建元件包括:启动子序列(山羊β-乳球蛋白基因5'端调控区);外源基因(人乳铁蛋白基因cDNA);标记基因;缺陷型逆转录病毒基因组。

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