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Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200232,ChinaAbstract: Nine samples from swine abortus were detected by RT-PCR with a pair of primers specific to NS1 gene of Japanese encephalitis virus.

采用反转录聚合酶链式反应方法检测猪流产胎儿病料中的乙型脑炎病毒,并对阳性病料做病毒分离与纯化。

Objective To explore the roles ofα-amanitin in the replication ofhepatitis B virus in HepG2 2.2.15 cell line Methods MTT assay were used to observe the cytotoxicity ofα-amanitin to the HepG 2 2.2.15 cell line.

中文摘要:目的:基于α-鹅膏毒肽的作用机制和乙肝病毒复制转录特点,初步探讨α-鹅膏毒肽对HepG2.2.15细胞的毒性及其对乙型肝炎病毒复制的影响。

Many technical measures have been taken as follows to ensure the safety management: a. operation steps of installing the cross joints are improved to guard against damage on metal sealing and the hanger; b. well logging is applied to gas testing so as to achieve early warning in a scientific way; c. the burning processing unit of QTI3000 is widely used to reduce the bad impact on the environment; d. the articulation of perforating gun is improved to alleviate labor intensity and shorten the perforating time so as to shut down the well promptly in case of any accidents.

在安全技术措施上,改进了坐完井悬挂器操作步骤,避免损坏金属密封,造成悬挂器损坏;将录井技术应用于试气作业,实现了科学预警;推广应用了QTI3000型焚烧处理装置,降低了对环境的不良影响;改进了射孔枪的连接方法,减轻了工人的劳动强度,缩短了起射孔枪时间,能在起钻发生井喷失控前及时控制关井。

Generally, B in the configuration closest cell's DNA conformation, it and the double helix model are similar. A-DNA and when RNA member double helix area as well as duplication forms the DNA-RNA hybrid molecule conformation is close. Z-DNA take the nucleotide dimer as the unit left-hand winding, its principal chain assumes the denticle the shape, therefore.

一般认为,B构型最接近细胞中的DNA构象,它与双螺旋模型非常相似。A-DNA与RNA分子中的双螺旋区以及转录时形成的DNA-RNA杂交分子构象接近。Z-DNA以核苷酸二聚体为单元左向缠绕,其主链呈锯齿形,故名。

A genechip analysis was performed using RNAs derived from embryos injected with squint mRNA, MZoep mutant embryos that are deficient in Nodal signaling, and wildtype embryos at the 30% epiboly stage Transcriptswith at least two-fold changes in expression level between wildtype and the other samples were identifyied In squint mRNA-injected embryos, 265 transcripts show anincreased expression level and 111 have a decreased expression level; in MZoep embryos, the expression of 1 495 transcripts increases while 550 transcripts express at a decreased level.

为鉴定受Nodal信号调控的基因,特别是那些转录因子基因,通过将来自squint过量表达、缺失Nodal信号的MZoep突变体或野生型30%外包期胚胎的RNA与Affymetrix斑马鱼寡核苷酸芯片杂交。

Provides cable connectivity from a laptop to any device with a PCIe x1 cable connector such as desktop storage devices, video monitors and add-in board expansion boxes.

提供从膝上型计算机的缆绳连通性给所有设备以一个PCIe x1电缆接头例如桌面存贮设备,录影显示器和扩展程序扩展箱子。

The way a film-maker might use a hand-held camera to give the impression of documentary urgency

就好像一个导演可能会用掌上型的录影机去摄影好给观众一种拍摄记录片时拍的很慌忙的感觉

Gena has successfully developed a quick molecular diagnostic system through which may simultaneously identify and differentiate kinds of different subtypes of influenza A viruses including H5N1 which highly makes illness.

我们开发出了一个可同时检测流感病毒多种亚型,包括高致病的 H5N1型的快速分子诊断系统,在一个管内采用多重逆转录 RT-PCR 法扩增可能潜在的流感病毒基因组的多个特异靶点, RT-PCR 产物可用 xMAP 技术检测和鉴别。

Objective To study the binding of HBV X protein to the general transcription factor ⅡB.

目的 研究乙型肝炎病毒X蛋白能否结合共同转录因子ⅡB。

Whether E2F1 can be used as a germinal center B cell DLBCL marker was also discussed.

同时对转录因子E2F1作为一种新的生发中心B细胞型DLBCL的判断指标的可行性进行探讨。

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Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

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