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Second, low glutelin content was regulated at the level of transcription, but the suppressed glutelin genes were not among those published.

采用定量RT-PCR方法对LGC-1及其野生型日本优中已知谷蛋白基因逐个进行表达分析,结果表明,GluA-1,GluA-2,GluA-3和GluB-1,GluB-2,GluB-4基因的转录水平在二个品种中无明显差异。

The total RNA was extracted from spleen of Xiphophorus helleri which had been immunized for two weeks. The core sequence was amplified by RT-PCR. The cDNA end sequences were amplified by RACE, and then the full-length cDNA of sIgM heavy chains gene were obtained by putting all of them. The full-length sequence contains the immunoglobulin signature sequence: FKCIANH.

以免疫两周的剑尾鱼为材料,提取脾脏总RNA,通过逆转录-聚合酶链式反应扩增出分泌型免疫球蛋白M(secretory immunoglobulin M, sIgM)重链基因的核心序列,再应用3'和5'快速扩增cDNA末端方法扩增其末端序列,拼接后获得剑尾鱼sIgM重链全长cDNA序列。

The comparative transcript profiles between OP and OR rats reveal some differentially expressed genes involved in lipids metabolism, ketone body production, blood pressure regulation and adipoctyes differentiation processes.The combined strategy of metabonomics and transcriptomics provides comprehensive information of metabolic characters of obesity and corresponding mechanisms to obese-related metabolic disorders from a systematic view, e.g.

高脂饮食喂养建立的OP大鼠体内的整体代谢特征与OR大鼠不同,具体包括:交感神经系统活性、能量代谢、脂肪酸代谢、氨基酸代谢以及肠道菌群结构等;OP和OR两种表型的转录组学比较也揭示了部分参与脂质代谢、酮体生成、血压调节和脂肪细胞分化等调控过程的基因存在差异性表达。

Many bHLH transcription factor of MYC mainly expressed in meristem, in the differentiation and development process of plant cell.

大量MYC型bHLH转录因子主要在分生组织中表达,参与植物细胞分化和发育过程。

Inducible and constitutive expression vectors for monocotyledonous plants were constructed and used to transfer into maize calli. Plants were differentiated and regenerated from the positive calli screened with gradient concentration of hygromycin. 4 transformed plants with inducible expression vector and 2 with con

PCR检测获得4株用诱导型表达载体转化的植株和2株用组成型表达载体转化的植株,为进一步研究抗逆相关转录因子基因分eb在单子叶中的表达调控,培育抗逆玉米材料打下基础。

Results Gene transcripts of key components of RAS were found in the esophageal musculature.

结果 RAS基因转录的主要元件在试管肌组织中被发现,免疫化学方法显示了肌束和血管壁中分离的血管紧张素2-1型受体染色。

Objective To analyze HBV drug-resistant mutations against nucleoside analogues at 12 reported sites in 340 patients with chronic hepatitis B.

目的 分析慢性乙型肝炎患者HBV逆转录酶基因与核苷类似物耐药相关的12个位点上的突变情况及其临床意义。

The baculovirus expression system, utilizing the strong polyhedrin gene promoter of the Autographa californica nuclear polyhedrosis virus , has become an important tool for protein production in the field of molecular biology.

由于核型多角体杆状病毒的多角体蛋白不是病毒复制必需的蛋白,利用转录多角体蛋白基因的强启动子可以高效表达外源蛋白,包括原核和真核基因编码的蛋白。

This product is not only the recordable walkman, but also comes with auto-reverse feature at a reasonable price.

这不止是一款可录型随身听,同时在一个合理的价位上提供了自翻带功能。

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上,本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39(HaNPV-VP39)基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区(GAL4-DBD),使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合,在酵母菌中表达DBD-vp39融合蛋白,该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

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Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来,英国,在此地早期居住的是凯尔特人,已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。

The almost sure central limit theorem is a pop topic of the probability research in recent years,because it has many actual applications in the random analogue.

中文摘要:几乎处处中心极限定理是近几十年概率论研究的一个热门话题。它之所以引起人们的注意是由于它在随机模拟方面的实际应用参见Fisher