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In order to construct a normalized cDNA silencing library, a novel procedure was adopted to reduce the abundant copy number and increase the rare copy number. Then the normalized cDNA was put into pTRV vector to construct a cDNA library and this cDNA library was screened with the technology of virus induced gene silencing.

然后把均一化之后mRNA反转录得到cDNA,再与基因沉默载体pTRV重组,最后把构建好的载体通过电转化的方法转入到GV3101农杆菌中,从而建立起破色期番茄果实均一化cDNA沉默文库。

The present studies showed that two cell populations were found in haemocytes: large cell with high granularity and small cell with low granularity by flow cytometry FCM on light scanttering pattern. Two distinct cell types were identified based on phase contrast microscope: one type of cell was dark and dioptric aberration, while the other was bright and dioptric strong. By Giemsa and H.E staining, cytoplasmic staining were heterogeneous and internal particles were obvious in one type of cell, while cytoplastic staining were homogeneous and internal particles were inexistent in the other type of cell. By transmission electron microscope, we found that the mitochondria, Golgi apparatus organelles were rich and internal particles were obvious in one type of cells, and contrary to the another cells.

流式细胞术光散射图谱显示血细胞被分两类,一类为颗粒度高的大细胞,另外一类为颗粒度低的小细胞;相差显微镜观察显示,血细胞可分为胞体暗、折光性差和胞体明亮、折光性强的两类; Giemsa和H.E染色显示细胞分为胞质染色不均一、胞内颗粒明显和胞质染色均一、胞内颗粒不明显的两类;透射电镜超薄切片观察显示,颗粒明显的细胞胞质内线粒体、高尔基体等细胞器较丰富,颗粒不明显的细胞胞质内细胞器较少;负染结果表明血细胞主要分为表面不光滑、突起明显和细胞表面光滑、突起较不明显的两类。

In the different zoning of xenoliths, molybdenum and tungsten contents is lower and homogeneous,and be lower than the world\'s average abundance; tungsten compared to molybdenum,the content more homogeneous in xenoliths, compared to mantle\'s average abundance more less. This explains the geochemistry incompatible character homophyly and diversity of molybdenum and tungsten determined their trend enrichment in crust in the course of the mantle evolution and magmatism, among molybdenum more trend enrichment in lower crust but tungsten in middle-upper crust.

包体不同带中,钼、钨的含量较低且较为均一,并低于世界均匀丰度;钨和钼相比,其在包体中的含量更为均一,相对于地幔均匀丰度更小,说明钨、钼的地球化学不相容性的类似性和差异性决定了它们在地幔演化和岩浆过程中倾向富集于地壳中,其中钼更倾向富集于下地壳,钨则更倾向富集于中上地壳。

The monolayers of LPSQ can be successfully transferred onto hydrophobic surface of silicon wafer or quartz plate to develop a very stable and uniform Y-type Langmuir-Blodgett film when using toluene as the spreading solvent at proper surface pressure.

在适当的表面压下,以甲苯为铺展溶剂该单分子层可以被成功地转移至疏水的硅片或石英片上形成均一稳定的Y-型LB膜,其紫外吸收强度在几个月内均保持不变,说明该LB膜具有良好的稳定性,AFM图像表明其形成了均一致密的膜。

The gel dynamics experiments showed that the gel formation law was similar with the other polymer systems, which wasrelated with dense surface under the soft coagulating condition and the thickening gel; the square of gel thickness was linear with coagulation time. The qualitative analysis of the surface puckers of fibers spun from wet spinning and dry-jet wet spinning was made. The positions where the die swell appeared were not the same and the die swell appeared at the air gap where the fiber would markedly been elongated and vary little in the coagulant, thus the surface of fiber spun from dry-jet wet spinning was more smooth. The radial component concentration gradient was responsible for the radial structure of PAN fiber spun from dry-jet wet spinning. The thicker surface with microvoid structure of

对湿纺和干湿纺进行比较表层沟槽的产生进行定性分析,原因在于孔口胀大区域出现在不同位置,在干湿纺中,溶液的孔口胀大出现在空气层,并在该区完成大部分形变,进入凝固浴后形变很小,纤维表面较为光滑;干湿纺PAN纤维径向结构存在差异,这与丝条内组分由于双扩散进行导致径向上存在浓度差异直接相关;凝固强度较大的体系,可以获得厚度较大且孔结构尺寸较小的皮层;含较少缠结的PAN原液在纤维成形后截面很圆整,但原液缠结浓度增大时成形后圆整度下降;PAN纤维成形是一个远离平衡的过程,凝固作用缓和的体系可以使成形过程靠近平衡,可获得更大尺寸的孔洞结构;原液中少量的非溶剂添加剂显著改变纤维径向结构,可获得孔洞尺寸更小和更为均一的径向结构,这与径向浓度差异的减小、分相时间的集中相关;PAN原液的成形纤维表面依赖于凝固强度;由AFM对相近凝固条件下成形的PAN膜进下

Their vertical growth are slow, and height are obviously lower than other centipedegrass, especially choiceness in texture and uniformity; leaves of P23. P2. P25 are slim and soft, texture of them are finer, appearances of P22. P24 are flat and soigne, uniformitiy of them are the best.

它们的垂直生长缓慢,而且株高明显低于其它假俭草,特别在质地和均一性方面表现尤为突出,其中P23、P24、P25的叶片细软,质地良好,P22、P24外观平坦整齐,均一性表现最好。

On basis of measurement of homogeneous temperature of inclusions, analysis and calculation of δ〓C and δ〓O, and SEPM analysis, the author has studied the forming temperature of authigenic quartz and carbonate minerals for the first time. Furthermore, the author points out that it is unreasonable to recover paleo-geothermal gradient directly using homogeneous and modern burial depth.

作者在包裹体均一温度测量、碳氧同位素测量和计算及电子探针分析的基础上,首次对吐哈盆地侏罗系自生石英和碳酸盐矿物的形成温度进行了研究,确定了这两种成岩矿物的形成温度,并指出直接用包体均一温度和地层现埋深恢复古地温梯度存在不合理性。

We already know that in practice, hexanaphthene does not resolve the pigments of flowers, it can only send the flowers into the formation and floating, qardam qardam, the hue hue, surface, will not resolve the isolation and pigments flocculation.

我们在践诺洋还来懂得,硅油不克不及处置颜料的发花,它只能使发花酿成浮色,酿成均一色相、均一色调的暗不天,处置不了颜料的别离和絮凝。

Among these three methods, principal component analysis is not necessary; a large amount of severe problems (such as the determination of the number of clusters) encountered in the delineation of hydrologically homogeneous regions arise in conventional clustering methods and the employ of discrimination analysis is not intuitional.

在这三种统计技术中,主成分分析并非划分水文均一区必要的步骤;大部份关於水文均一区的划分的问题则存在於传统的群集分析方法中;而识别分析在水文均一区划分中的用法则并不直觉。

Nitrogen distribution in soils is highly heterogeneous. One way for plants to adapt to this soil character is proliferating more laterals around the N-rich sites so as to acquire N efficiently.

土壤中氮素的分布具有很大的不均一性,植物根系对这种不均一性的主要反应之一是在氮素富集区形成更多的侧根,从而高效获取这些养分。

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Methods: Five patients with parkinsonism or dystonia were assigned to general anesthesia using an modified endotracheal tube.

本实验依照人体实验之相关规定进行,五位患有帕金森氏症或肌张力异常的病人接受神经立体定位手术。

If you can benefit from this book, it is our honour.

如果您能从本书获益,这将是我们的荣幸。

The report also shows that the proportion of unmarried men and women living together has doubled between 1986 and 2006, with 13 per cent of those aged 16 to 59 now cohabiting.

报告还指出,从1986年至2006年,英国未婚男女同居的比例增长了一倍,在16岁至59岁的人群中,有13%的人同居。