含氨的

It is the fact that three methionines distributed in the Hsp16.3 will begradually oxidated, in vitro, by hydrogen peroxide(H2O2). When the hsp16. 3 gene was cloned with pMV261 plasmid vector and transfected into Mycobacterium smegmatis, we found that the ability of anti-H2O2 will be decreased greatly among the strains of M. smeg. containing pMV261-hspl6. 3 while compared with the control strains of M. smeg. containing free plasmid vector. But we found that the ability of anti-H2O2 will be increased greatly among the strains of M. smeg. containing pMV261-38kDa while compared with the same control.

尽管Hsp16.3中有三个甲硫氨酸在体外能被过氧化氢(H_2O_2)逐一氧化，我们将Hsp16.3基因克隆到pMV261质粒上，并转染到耻垢分枝杆菌中发现，转染了pMV261-16.3的耻垢分枝杆菌抗过氧化氢的能力大大下降，而对照含pMV261-38kDa的耻垢分枝杆菌抗过氧化氢的能力却有明显的增强，据此，我们认为，Hsp16.3在细胞内可能不具有抗过氧化氢的能力。

The results showed that the content of Se element in the Se-MT was 2.5 folds higher than that of Zn-MT, on the contrary the content of sulfur element in Se-MT was 2 folds lower then Zn-MT, C, H, N contents in MT were no significant differences in both groups. In terms of chemical property, the element of selenium is very similar to sulfur because they are in the same group in the periodic table. The above results illustrated that the Se could replace S from SH-sulfhydryl to SeH-selenihydryl in cysteine, which presents the existence of a novel Se-MT.

结果表明Se－MT中Se元素含量高于Zn－MTZ.5倍左右，而 S元素含量低于 ZnMT 2倍左右，C、H、N元素含量无明显差异，这说明了麦硒康诱导后Se仙T分子结构上的差异，由于Se元素与S元素同属氧族元素，在化学性质上极为相似，故Se元素极可能替代了MT的合成过程中所必需半味氨酸残基中的SH一基因上的S元素成为SeH一基团，这样我们就得到了一种含SeH一基团的新型Se－MT。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分，第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究，确立了一种较好的培养方法：与颗粒细胞共培养，找到了一种适合猪卵母细胞体外成熟的培养基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%，国外报道的最高成熟率为(85.7±4.1)%；第二部分对猪卵母细胞孤雌激活的化学方法进行了研究，确立了化学激活猪卵母细胞的两种最佳方法：1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min，再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中，卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min，再与8mM的DTT共孵育30min，卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%；第三部分对孤雌激活胚胎的培养条件进行了研究，确立了一种最佳的胚胎培养条件：在SOF简单培养基中添加颗粒细胞进行前3天的培养，然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养，其桑椹胚和囊胚的发育率为(59.5±3.2)%；第四部分研究了IGF-I

Green eggs with a shell of the egg is different from other nutritional content and structure of the green shell of the egg yolk than Cao Ji 8% of the large egg yolk, egg white thick, easily digested and absorbed by the body, egg yolk pigment Roche up to 13 About class (ordinary egg yolk pigment Roche for the general level 4 -5), lecithin, a living -A, B, E, amino acid content than the 5-10 times higherthanordinary eggs, the yolk of the egg ball ammonia β White and γ-globulin were higher than other kinds of chicken, the best is good tonic, Green Egg high-quality, nutritious, high-selenium, high zinc, high-PC PE, high Amino acid, high-vitamin A, E, the high non-saturated fatty acid, low-fat, low cholesterol, high Flavin light, the lightpigment αβγ and unknown immune globulin antibodiesanddiseaseresistantfactorisextremely desirable Health food, the scientists as "the ginseng eggs."

绿壳蛋具有不同于其它蛋品的营养结构和含量，绿壳蛋的蛋黄比草鸡蛋黄大8%，蛋清稠，极易被人体消化吸收，蛋黄色素可达罗氏13级左右（普通鸡蛋的蛋黄色素一般为罗氏4级-5级），卵磷脂、维生素A、B、E、氨基酸的含量比普通鸡蛋高出5-10倍，蛋黄中的氨β球蛋白和γ球蛋白均高于其它鸡种，是上等滋补佳品，绿蛋蛋品质高，营养丰富，含高硒、高锌、高卵磷脂脑磷脂、高氨基酸、高维生素A、E、高不饱合脂肪酸，低脂肪、低胆固醇、富含光黄素、光色素αβγ球蛋白和未知免疫抗体和抗病因子，是极为理想的保健食品，被科学家誉为&鸡蛋中的人参&。

In a product free of citric acid or citrates, for oral ingestion, the taste of arginine is counteracted by lactic, malic or adipic acid; in a formulation for tube-feeding, citric acid and citrates in a specified ratio controls the product's stability.

在不含柠檬酸或柠檬酸盐的用于口服的产品中，通过乳酸、苹果酸或已二酸来抵消精氨酸的味道；在用于管饲的制剂中，以特定比例的柠檬酸和柠檬酸盐控制产品的稳定性。

Cell lines were co-tranfected with bi-plasmids and cultured at 1% O〓 condition, luciferase assay demonstrated that all of the expression systems with different domains could be hypoxia-activated. GAL-CAD achieved more than 100-fold induction in A549 cell and more than 40-fold in HepG2; expression level of the system markedly increased after addition of glycin arm, almost 10-fold enhancement in HepG2 cell. Because basic expression also increased, the induction fold by hypoxia decreased.

双质粒共转染不同细胞株后，在1％O〓环境下培养24h，荧光素酶活性检测结果提示含不同结构域的表达系统均有受缺氧激活的能力，其中GAL-CAD在A549中获得100倍以上的诱导，在HepG2中获得40倍以上的诱导；加入甘氨酸臂后该表达系统的转录活性大为增强，在HepG2细胞中增强了10倍以上，但是由于基础表达也有所增加，受缺氧诱导的倍数下降。

I'm strongly against the death penalty — it's an eye for an eye.

我不赞成死刑——这是以牙还牙的报复行为。

And to get you the support you need, we're enlisting all elements of our national power: our diplomacy and development, our economic might and our moral suasion, so that you and the rest of our military do not bear the burden of our security alone.

并给你们所须的支援，我们正徵召国家所有各种的力量：我们的外交及发展，我们的经济力量与道德劝说，所以你们与其他军人不须要孤独地负起国家安全的责任。