可诱导的

The direct incorporation of U-13C glucose was estimated by the intensity increase of m/z to F(F was parent fragment, and n was the carbon number in the fragment), while the total isotope incorporation from the added 13C could be calculated according to the abundance ratio increment summation from m/z (Fa+1) through (Fa was the fragment containing all original skeleton carbons, and T was the carbon number in the amino acid molecule). The 13C enrichment in the target compound was expressed as atom percentage excess, and that of D-amino acid needed to be corrected by the coefficient of hydrolysis-induced racemization. The 13C enrichment reflected the carbon turnover velocity of individual amino acid enantiomers, and was powerful to investigate the dynamics of soil amino acids.

外加葡萄糖直接合成氨基酸的比例可利用质谱碎片的相对强度变化来评价（n为质谱碎片F中含有的碳原子数目）；而源于葡萄糖的13C同位素在氨基酸分子中的富集程度是所有同位素峰相对强度变化的总和。13C在目标化合物中的富集比例用原子百分超评价，D氨－基酸的APE还需进一步利用水解诱导的外消旋化系数校正。13C在目标化合物中的富集程度可反映各氨基酸手性异构体的碳循环速率大小，是研究土壤氨基酸动态变化的有力工具。

So, Z-1 was named IEM106 and regarded as a live oral cholera vaccine candidate eliciting both antibacterial and antitoxic immunity.

以上结果表明，Z-1无毒，免疫家兔可诱导抗菌抗毒免疫，具有很好的保护作用，可以作为霍乱弧菌抗菌抗毒口服活菌苗候选株，命名为IEM106。

Japonicus, but also displayed the inhibition-effect, i.e., the change of MT levels in the copepod was correlated with metal concentration and duration.

在12 d的暴露过程中，镉或镍既可诱导日本虎斑猛水蚤MT的合成，也可表现为抑制效应，即MT的变化与暴露剂量和时间均有相关性。

By Percoll fluid and density gradient centrifugation,the MSC was obtained;after the cells filled the bottom of vessel,subcultured them,when they subculture in third generation ,redigested them,500 R/min centrifugate,alter the

经percoll液密度梯度离心法能获得纯度较高的人骨髓间充质干细胞，这种细胞在体外培养体系中保持旺盛的增殖能力；经轻度离心和化学限定培养基法可诱导骨髓间充质干细胞向成软骨方向分化，增殖旺盛，可传一代培养，是软骨组织工程理想的种子细胞。

MSC can be obtained by percoll fluid and density gradient centrifugation, this cells kept proliferation and differentiation ability in vitro; MSC can differentiate into chondroblast by lightly centrifugation and chemical definition medium, after one generation, the differential cells kept the phentype of chondroblast, they are a kind of ideal seed-cells for cartilage tissue engineering.

经percoll液密度梯度离心法能获得纯度较高的人骨髓间充质干细胞，这种细胞在体外培养体系中保持旺盛的增殖能力；经轻度离心和化学限定培养基法可诱导骨髓间充质干细胞向成软骨方向分化，增殖旺盛，可传一代培养，是软骨组织工程理想的种子细胞。

In region conservational development, the aim for landscape ecology evaluation is to make good use the human factors which can induce and improve the landscape ecology, to curb and to prevent the human factors affection which can lead to the reduction of the landscape ecosystem, to establish the nature-human compound landscape ecosystem with reasonable structure and highly effective function.The fenshuiling-dajiangou-jiuqu region, an important ecological protection area of Shandong Province, lies in the southward hilly region in Jinan.

在区域保护性开发建设中引入景观生态评价，旨在区域开发建设过程中充分发挥可诱导和促进景观生态系统进化的人为因素，抑制和防止可导致景观生态系统退化的人为因素的影响，建设结构合理、功能高效且适合人类生存的自然-人工复合景观生态系统。

CaN Aβ gene silencing can reduce myocardial hypertrophy in cultured cells, si1280 (21bp) of CaN Aβ gene is the most effective target site for siRNA. The method of intrapericardial injection of plasmid, microbubbles and erzymes can improve transfection efficiency of non-viral plasmid with satisfying targeted transfection. But the scope of transfected myocytes is still limited. CaN Aβ shRNA expressing plasmid transfection in vivo by pericardial injection results in decreased CaN Aβ protein expression of small part of myocytes, and CaN Aβ mRNA only shows decreased trend. The dosage of non-viral vector and the parameters of ultrasound energy should be optimized in further study.

结论RNAi技术抑制CaN Aβ基因表达可有效减轻Ald诱导的心肌细胞肥大程度；CaN Aβ基因中si1280(21bp)片段为实现RNAi的更有效片段；微泡+酶类心包腔内注射超声导入的方法可有效改善非病毒载体在体心肌的转染效率，同时具有一定的靶向性，但总的转染范围仍然有限；采用这一方法进一步进行&一肾一夹&心肌肥大模型大鼠在体心肌细胞的CaNAβ的RNAi干预，发现心肌肥大大鼠心外膜下局部心肌细胞CaN Aβ蛋白水平降低，CaN AβmRNA水平虽有下降趋势，但无统计学差异，提示质粒的用量及超声导入的参数有待进一步研究使其优化。

The ability to modulate the expression of multiple genes by tetracycline and its derivatives is likely to regulate the timing and level of gene expression, quantitatively induce the expression of toxic protein, increase therapeutic efficacy and safety, and provide a safe and regulatable way for gene therapy.

CHO-Tet-On细胞株的建立，可利用四环素及其衍生物调节多种外源基因的表达，有效调控基因表达的时间和水平，定量诱导毒性蛋白的表达，增加治疗的疗效和安全性，有望为基因治疗提供一条可控的安全途径。

2Radiofrequency ablation with anchor-shaped electrodes could induce coagulation necrosis of most of the VX2 nodules in rabbit lung, and could induce apoptosis of tumor cells; The course of pathological changes of ablated area was surrounded by organization, the ablated area would be encapsulated by fibrosis tissue.

2采用锚状电极RFA治疗兔肺内VX2肿瘤，其基本病理改变为：绝大多数肿瘤病灶的凝固性坏死，进一步的改变为凝固性坏死区被机化包裹，可有钙盐沉积；RFA可诱导肿瘤细胞凋亡。

This paper endogens carbon tax and subsidy two variables, discussing how to decide the optimal level of carbon tax and subsidy. The results of the research are obtained as the following:(1) price and capital subsidy which one is priority depend on the productivity of renewable energy capital, if productivity is high enough, then should adopt capital subsidy policy;(2) price and capital subsidy indeed exist trade-off and non-independent relationship;(3) renewable energy regulation ratio has negative effect on carbon tax, however has positive effect on price subsidy;(4) green certificate price increase will result in decrease carbon tax and price subsidy, but increase capital subsidy;(5) if the authority loose the environmental target, then should impose high carbon tax which maintains the environmental quality, but have ambiguous effect on subsidy policy;(6) increasing stability of renewable energy, then increase price subsidy, however, under budget constraint should decrease capital subsidy;(7) analyzing Taiwan's three wind power energy with different price subsidy, which can improve the technology of wind power energy. In another word, it can raise the capacity of wind power energy and reach the goal of renewable energy.

本文考量政府预算限制下，探讨最适碳税税率与补贴率订定之问题，获得如下发现：(1)发电量补贴与资本补贴对促进再生能源发展效果不一样，决定於再生能源资本的生产力，如果资本生产力足够高，则发电量补贴效果较佳；反之，则应优先采行资本补贴措施；(2)发电量补贴与资本补贴的确存在抵换而非独立关系，亦即在政府预算限制下，为促进再生能源发展，应将种种补贴措施整合考量，以最低行政成本达到再生能源发展目标；(3)再生能源管制配比与碳税税率及资本补贴率呈反向变化关系；而与发电量补贴呈正向关系；(4)绿色权证交易制度具促进再生能源管制目标达到之奖励效果，因此，绿色权证价格提高可降低发电量补贴及碳税税率，降低碳税对经济不利冲击效果；(5)政府如果采行较宽松环境目标，宜提高碳税税率，避免环境品质进一步恶化，然而，对发电量或资本补贴之影响效果不确定；(6)再生能源发电效率愈高，则应提高再生能源发电补贴，在预算限制下，应降低再生能源资本补贴；(7)透过台湾三座风力机组的实证分析，采取差异性发电量补贴，将可诱导较高效率风力机组的投资行为，提高整体风力发电效率，达到再生能源发展之目标。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。