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Citric acid production with cellulase hydrolyte of wheat stalk by Aspergillus niger , the effects of media component, fermentation at constant temperature, and at periodic variable temperature on citric acid were studied.

以麦草纤维素酶解液为原料,用黑曲霉发酵制备柠檬酸,研究了培养基组成、恒温发酵和周期变温发酵对柠檬酸发酵的影响,结果表明,适宜的培养基组成为NH4 NO3 0 。3%,KH2 PO4 0 。1%,Mg2 + 30 0× 10 -6,Fe2 + 10×10 -6,此时,柠檬酸产量为 10 。5 2 g/L ,糖转化率为 6 0 。80 %。

Moreover,no difference was observed between trace elements added and not added into the medium,which proved that the trace elements contained in Jerusalem artichoke tubers are sufficient for 2,3-BD production.On the basis of the results,separate hydrolysis and fermentation and simultaneous saccharification and fermentation were performed,respectively.

通过摇瓶发酵实验考察了不同底物、不同水解方式以及微量元素对发酵的影响,结果表明,菊芋是良好的发酵生产2,3-丁二醇的底物,采用酸水解或酶水解两种方式制得的糖液发酵效果基本一致,菊芋中含有的微量元素完全能够满足发酵的需要,发酵过程中无需添加微量元素。

The study on different consislency bone paste liquid fermented by lactic acid bacteria showed that the conteat of Ca ++ considerably increased. The conteat of Ca ++ in 10% of fermented bone paste was 8 times higher than that of unfermented bone paste; in 20% of bone paste, 5 times higher.

通过用乳酸菌对不同浓度的骨泥进行发酵发现,发酵骨泥液中游离钙的含量明显提高。10%的骨泥经乳酸菌发酵其游离钙的含量比未经发酵的鲜骨泥提高8倍,20%的骨泥经发酵后比鲜骨泥提高5倍。

The results showed that the use of tourmaline could increase alcoholic fermentation speed of molasses, the fermentation speed kept increasing with the increase of the use level of tourmaline, however, as its use level was above 11 %, the increase of fermentation speed was inevident; the use of tourmaline had no side-effects on the content of the main impurities including methanol, n-propyl alcohol, isobutyl alcohol, isoamyl alcohol; though it had weight loss after a period of use, it could still promote alcoholic fermentation of molasses.

结果表明,电气石对提高酵母酒精的发酵速率有促进作用,随着使用量的增加,发酵速率也随着提高,当使用量超过11 %时,发酵速率的继续提高不明显;电气石对发酵过程主要杂质甲醇、正丙醇、异丁醇、异戊醇等没有影响;电气石在使用一段时间后,重量减轻,但仍有促进发酵的作用。

Under the studies on conditions of liquid fermentation of Poria cocos,the techniques of Erlenmeyer flask additional medium liquid fermentation and additional medium liquid fermentation in fermentor were studied in this paper.

本文在茯苓摇瓶液体发酵条件研究的基础上,进一步探讨了茯苓的摇瓶补料培养液体发酵和发酵罐补料液体发酵的发酵工艺。

Studied the effect of quality of the one step liquid to the second step fermentation rate and periods first time, confirmed the effect factor of the one step leavens process versus the second step.

首次研究了从一步发酵工艺的醪液质量对二步发酵的收率及周期影响,并确定了一步发酵工艺对二步发酵工艺影响因素。

With saccharified liquid of corn meal and cassava starch as a material respectively, the effects of corn steep liquor concentrations on glycerol fermentation by osmophilic yeast were discussed.

然后分别以玉米粉和木薯淀粉糖化液为原料,用耐高渗酵母发酵生产甘油,研究了玉米浆对二者甘油发酵的影响并对二者进行了比较,结果表明:当玉米粉和木薯淀粉糖化液还原糖含量分别为 2 5 %,尿素为 0 。2 %,pH为 4 。5时,用玉米粉糖化液发酵甘油时可不添加玉米浆,甘油产量最高可达 2 %,而用木薯淀粉糖化液发酵甘油时,适宜的玉米浆为 0 。15 %,甘油产量最高可达 4 。9%。

Based the optimized batch-fermentation condition, it was confirmed that the DE of cassavahydrolysate in the fed batch culture was 80, 4%(NH4)2SO4, adding fed batch culture four times 36hours after the fermentation beginning, and 1.5 hours of fed interval, were the optimized fed-batchfermentation conditions, by studying the culture composition, method, original time and interval offed batch, and some main parameters of the citric acid fermentation, such as citric acid yield, invertrate, fermentation periods and residual glucose.

在批式发酵优化条件的基础上,通过对补料液的组成、补料方式、流加起始时间、流加间隔时间等对柠檬酸发酵过程的主要参数,如产酸率、转化率、发酵周期、残糖等的影响进行了研究,确定了柠檬酸补料发酵的优化条件,即:补料液中木薯糖化液的DE值为80,加入0.04%(NH4)2SO4;发酵36h小时开始分四次流加补料液,流加的间隙时间为1.5小时。

From black song fungi with produce the Ruan false silk yeast fungus to constitutePass to design single factor and was hand over to experiment to make sure KB and H Ruan hybrid the fungi ferment of the best condition, test result enunciation:The KB is in N source 5%, anticipate water to compare 1:2, inoculate to compare 1:1, inoculate to measure 20%, pH to be worth 3.5, ferment time for 5 days, ferment to connect to go in to inoculate to measure to 20% H Ruans to mix the germ stub as hybrid fungi on the 3rd day the best ferment a condition, in that case, the Chinese herbal medicine residue true egg white content after ferment is 17.26%, true egg white growth rate is 82.39;The thick fiber content is 26.09% and decline the solution rate is 14.54%.

通过设计单因子以及正交试验确定了KB及H朊混合真菌发酵的最佳条件,实验结果表明:KB在N源5%、料水比1:2、接种比1:1、接种量20%、pH值3.5、发酵时间5天,发酵第3天接入接种量为20%的H朊混合菌株为混合真菌最佳发酵条件,在该条件下,经过发酵后的中药渣真蛋白含量为17.26%,真蛋白增长率为82.39;粗纤维含量为26.09%,降解率为14.54%。

Natural fermentation of corn in the process of lactic acid bacteria as the most important advantage of strain, the article on lactic acid fermentation process in detail the analytical results show that: from chemical constituents, the ferment of lower ash, soluble sugar, fat content, protein, starch proportion cases raising; from the structure of starch, amylose fermentation in the increase, in molecular weight amylopectin rice amylopectin close to the molecular weight of the development.

在玉米米粉制作的过程中,发酵的方法能改良玉米的性质使其具有可加工性、改善玉米的不良风味。玉米自然发酵的过程中,乳酸菌为最主要的优势作用菌种。对乳酸菌发酵的过程进行详细的解析表明:从化学成分上来讲,发酵作用降低了灰分、可溶性糖、脂肪的含量,使蛋白质、淀粉所占比例提高;从淀粉结构来讲,发酵使直链淀粉含量增加,支链淀粉分子量朝着接近大米支链淀粉分子量的范围发展。

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