动物化学

Of the late two years,in order to find out a kind of dental implant ing materialwhich would possess both the very good properties of biological compatibility,the chemical compatibility and the mechanical compatibility,an animal experi-mental preselection was accomplished by our research team with six kinds of im-planting material chosen from four main sorts of oral implant ing material(met-al,ceramic,high polymer and carbon),As the result of the Preselection,thematerial……bio-ceramic was chosen by ...

为了寻求生物相容性、化学相容性和力学相容性均好的种植材料，在口腔四大类种植材料(金属、陶瓷、碳素、高分子)中，选用6种材料作了动物实验预选。根据预选结果，选定以生物陶瓷为主攻方向，从而研制成功锆磷灰石陶瓷，以之进行了理化性能、生物学性能、及动物实验考查，合平种植要求。并以考查结果与钛合金、多晶氧化铝、单晶氧化铝、钛合金喷涂氧化铝、低温各向同性碳、聚砜种植作了比较，主要性能均较其它几种比较材料为优。经临床初试，锆磷灰石陶瓷的种植效果也较好，其界面为骨性结合。

The method inducing periodontitis animal model contains biological,physical,chemical,and various kinds of environment factors.

建立牙周病动物模型的方法，主要是用生物的、物理的、化学的和各种环境因子作用于实验动物。

It is respectively: Production, facility for transporting operates personnel: Jockey of lathe work, milling, grinding work, boring labour, solderer, modular machine tool, machining center jockey, casting matchs benchwork, besmear to install ship-fitter of ship-fitter of equipment of benchwork of labour, tool, boiler, electric machinery, discretion to control ship-fitter of appearance of ship-fitter of appearance of instrument of electric equipment ship-fitter, electron, electrical engineering instrument, machine; Irrigation works of aricultural herd fishing produces personnel: Inspector of quarantine of the member that animal epidemic disease prevents and cure, animal, firedamp produces work; Personnel of line of business of commerce, service: The member that assistant, bagman, publication is issued, Chinese traditional medicine purchase and sale, clerk of the member that the division of division of appraisal appraisal division, Chinese style noodle, Chinese style noodle, Western-style

分别是：生产、运输设备操作人员：车工、铣工、磨工、镗工、焊工、组合机床操作工、加工中心操作工、铸配钳工、涂装工、工具钳工、锅炉设备装配工、电机装配工、高低压电器装配工、电子仪器仪表装配工、电工仪器仪表装配工、机修钳工、汽车修理工、摩托车维修工、精密仪器仪表修理工、锅炉设备安装工、变电设备安装工、维修电工、计算机维修工、手工木工、精细木工、音响调音员、贵金属首饰手工制作工、土石方机械操作工、砌筑工、混凝土工、钢筋工、架子工、防水工、装饰装修工、电气设备安装工、管工、汽车驾驶员、起重装卸机械操作工、化学检验工、食品检验工、纺织纤维检验工、贵金属首饰钻石检验员、防腐蚀工；农林牧渔水利生产人员：动物疫病防治员、动物检疫检验员、沼气生产工；商业、服务业人员：营业员、推销员、出版物发行员、中药购销员、鉴定估价师、中式面点师、中式面点师、西式烹调师、西式面点师、调酒师、营养配菜员、餐厅服务员、前厅服务员、客户服务员、保健按摩师、职业指导员、物业指导员、物业治理员、锅炉操作工、美容师、美发师、摄影师、眼镜验光员、眼镜定配工、家用电子产品维修工、家用电器产品维修工、照相器材维修工、钟表维修工、办公设备维修工、保育员、家政服务员、养老护理员；办事人员和有关人员：秘书、公关员、计算机操作员、制图员、话务员、用户通信终端维修员。

Animals after chemolysis failure to homogenate respectively,centrifugate and take the pure liquid,mcasure collagenase activity with Blanckaert method,the data handled with statistics did not show obvious difference.

组织化学检测：将实验动物组、对照组、手术组和化学溶解术失败再手术组标本的纤维环内层、中层和外层分别匀浆、离心，取上清液，按Blanckaert法测定胶原酶，所得据经统计学处理差异无显著性。

Thunberg Fritillary Bulb is one of the most popular Traditional Chinese herbs. Some trials in vivo and animals indicated that Peimine and Peiminine, extracted from Thunbery Fritillary Bulb, have the function of reversing MDR of two different kinds of mechanisms. They can improve the cytotoxic of ADR to cancer cells by 5～10 folds under concentration of 10% cancer cell growth inhibitory rate. Peimine and Peiminine not only feather typical character of modifier of MDR, but also differ from other moldifiers such as Calcium channel blocker, Cyclosporines in chemical structure, they belong to cevine groups alkaloid, a subgroup of C-Nor-D-homosteroid alkaloid.

浙贝母是临床常用中药，体外及动物试验证明，浙贝母中的有效活性成分贝母甲素和贝母乙素具有逆转肿瘤细胞MDR的作用，而且能逆转两种不同机制的多药耐药肿瘤细胞的耐药性，在无明显细胞毒剂量下，可使阿霉素对耐药肿瘤细胞的杀伤活性提高5-10倍，不但具备明确的多药耐药逆转剂的特征，而且在化学结构上属异甾类生物碱中的瑟文类生物碱，完全不同于钙离子拮抗剂、免疫抑制剂和其它现有的耐药逆转剂，具有作用靶点宽、化学结构独特的特点。

Samples were extracted 6 and 12 weeks after operation for histological, histochemical and immunohistochemical analysis.

术后 6周和 12周分别处死动物、取材，进行缺损区大体、组织学、组织化学和免疫组织化学分析。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

This survival time was not significantly altered by bilateral denervation of cervical vagi (the major sensory system for detecting changes in pulmonary conditions), by bilateral denervation of peripheral arterial chemoreceptors (the major sensory system for detecting changes in arterial blood gases and pH), by pretreatment with dimethylthiourea, or by pretreatment with milrinone (a phosphodiesterase Ⅲ inhibitor that may avoid respiratory muscle fatigue).

而截断双侧颈部迷走神经（负责侦测肺内部情况的最主要感觉神经）、摘除双侧的周边化学接受器（负责侦测动脉血液气体及酸碱值的最主要化学接受器）、或投予Milrinone（一种phosphodiesteraseⅢ抑制剂，藉抑制cAMP被分解，以增加细胞内cAMP的量，可以使疲乏的呼吸肌内再增加收缩力）、Dimethylthiourea等药物的动物，都无法延长其存活时间。

At these nadirs, chemosynthesis—the thermal and chemical energy given off by deep-sea vents—gives life to creatures like the eelpout fish and tubeworms pictured here.

在这些nadirs ，化学合成-热和化学能源鉴于深海喷口――生命的动物一样， eelpout鱼类和管虫图案在这里。

In our research,Wistar rats and SD rats which were parathyroidectomized were used as donors and recepients respectively.The parathyroid gland of the Wistar rat were allografted into the cerebroventricle and other different sites of the brains in SD rat s tereotaxically. Using methods of testing serum Ca~(2+) concentration,histological examination,histochemical and immunocytochemical methods and electron microscopic observation,we investigated the survival of the grafting tissue,its revascularization, infiltrating of monocytes, changes of microglia and astrocytes, the relationship of the CNS with the immune system, and the mechanism of rejection were investigated.The results as following: I .Effects on PTG after allografting into cerebroventricle and cerebral parenchyma of the brain: Testing serum Ca~(2+) conc.

本研究以Wistar大鼠作为供体，切除甲状旁腺的SD大鼠作为受体，在立体定位下将同种异体的PTG移植到大脑的不同部位，设计了侧脑室-内分泌系统移植的代替途径，比较了侧脑室内移植和脑实质内移植的异同，通过测定血清Ca~(2+)的浓度、组织学检查、组织化学和免疫细胞化学以及电镜观察对移植后PTG组织的存活、血管的再生、单核细胞的浸润、CNS内胶质细胞的变化、对免疫系统的影响及免疫排斥的机制进行了研究，结果如下：一、同种异体PTG侧脑室内移植与脑实质内移植的比较：血清Ca~(2+)的检查发现，PTG侧脑室内移植后第7天，血清Ca~(2+)的浓度恢复到8mg％以上，一直持续到移植后第8-12周；移植后第12周仍有60％移植PTG的动物血清Ca~(2+)维持在正常水平。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。