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动物化学

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Comparison of four annona cultivars;2. Advances in the Studies on Postharvest Physiology and Fresh-keeping Methods of Annona Fruits;3. Plants of Annonaceae and their chemical components have very s.

番荔枝科植物及其化学成分具有很好的抗肿瘤作用,体外实验研究显示强大抑瘤效果,而体内实验不多,为此以番荔枝总提取物进行了防治肝癌的整体动物实验。

Methods The animal model of optic nerve injury at the canalicular segment were produced in healthy adult rabbits.

建立家兔视神经损伤的动物模型,利用免疫组织化学方法观察管内段视神经损伤后GAP-43的表达。

The results are as follows: Both genistein and CNQX attenuated the ventilatory responses of peripheral chemoreflex, while no changes occurred following daidzein. The amplitude of integrated phrenic nerve discharge and the phrenic burst frequency were decreased by (-21.77±6.93%) and (-24.70±7.61%) respectively after administration of genistein.

结果显示,吸入低氧混合气后,动物呼吸加深加快;在NTS 处微量注射CNQX或genistein 都会不同程度削弱外周化学感受性反射引起的通气反应,而微量注射daidzein后对反射没有影响。

This paper reviewed detailedly chemical structure,physical and chemical characteristics,relation of structure and effect,toxicity of chloramphenicol,metabolism and residual in animal body,measurement method of residual of chlor...

本文就氯霉素的化学结构、理化特性、构效关系、毒性作用,在动物体内的代谢与残留,检测方法中的筛选法、定量测定法、确证法等进行了详尽阐述。

Methods:The animal models made through implantation from combined bacteria, were randomly into clysis group and the oral group, which were then detected the changes in the patho-morphology, of the oviduct by means of optical and electronic microscopes and the content of sICAM-1 in the serum measured with ELISA and the expression of ICAM-1 protein in tissues of oviduct measured with immunohistochemistry.

用&混合菌接种法&制作大鼠慢性输卵管炎性不孕动物模型,以妍婷颗粒对模型大鼠进行治疗,分为灌肠和灌胃两组,治疗后观察光镜和电镜下输卵管病理形态学改变情况,用ELISA法检测血清中sICAM-1水平和免疫组织化学方法检测输卵管组织中ICAM-1表达。

Methods: 30 adul male Sprague-Dawley rats were divided randomly into model group and control group. The rats were injected into lateral ventricle with coriaria lactone (50g/kg). The sections of the brains were processed by immunochemistry with antibodies against SYN and GFAP.

成年健康雄性SD大鼠30只,随机分为模型组及对照组,采用马桑内酯(50g/kg)侧脑室注射法建立急性癫痫动物模型,应用免疫组织化学方法观察癫痫持续状态下SYN和GFAP的表达。

After varied survival periods, the model mice were sacrificed to get the specimens of SN fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry to label the DA neurons and Calbindin D28k-containing neurons with the antibodies against tyrosine hydroxylase and CR respectively. The labeled cells were counted under microscope and analyzed statistically.

模型动物存活1周后,一部分取其中脑黑质节段,固定、包埋做连续冠状石蜡切片,以免疫组织化学染色方法,显示各组酪氨酸经化酶和钙结合蛋白表达阳性细胞,光镜观察并细胞计数,统计学分析;另一部分取其中脑黑质组织匀浆,行TH、CB的Westem blot免疫印迹方法检测,用LabWorks软件分析处理。

The mice were killed 3, 7 and 14 days after operation (8 mice per group at a time), the tissue of cystiform was harvested to receive gross, histology and immunohistochemistry observation, as well as RT-PCR and Western blot detection.

术后观察动物一般情况,于给药后3、7 及14 d,每组取8 只小鼠背部囊壁组织行大体、组织学观察及免疫组织化学、RT-PCR、Western blot 检测。

The experimental vitiligo models were prepared by chemical decolour method in Guinea Pigs. And we choose the Meladinines recommeded level (0.15%) as high concentration, use the high, middle and low concentration levels (0.15%, 0.075 %, 0.03 %) of LMOP gels to progress the pharmacodynamics comparative study, 8-MOP tincture was used as controll group, observing the melanin distribution, the number of DOPA active cells, the number of hair follicles contented melanin, the levels of ChE and MDA of the experimental vitiligo models.

用化学脱色法制备豚鼠实验性白癜风动物模型,以市售敏柏宁酊的推荐使用浓度(0.15%)为最高浓度,设定LMOP凝胶低、中、高三个浓度组(0.03%,0.075%,0.15%),进行药效学比较性研究,以8-MOP酊剂(敏柏宁酊稀释至含8-MOP 0.15%)为对照,考察其对皮肤黑素分布、DOPA阳性细胞数、含黑素毛囊数、胆碱酯酶及丙二醛的影响。

The pressor effect of adenosine was due to carotid body cheoreceptor activation. However, a slight increase of ABP induced by intracarotid bolus injection of adenosine following the section of carotid sinus nerves was observed in a few rats, which implied that the central effect might be involved in the pressor effect of adenosine. The delayed effect of adenosine may be attributed to the direct actions (including the negative chronotropic action, thenegative dromotropic action, the negative inotropic action and the vasodilator effect) of adenosine on the heart and blood vessels.

前一效应主要与颈动脉体化学感受器的激活有关,但在少数动物,颈动脉内注射腺苷的升压效应在切除窦神经后并未完全消除,表明腺苷有可能进入神经中枢而发挥作用;后一效应可能由腺苷直接作用于心脏和血管,通过其负性变力、变时、变传导和血管扩张等作用,导致心脏自律性降低,输出量减少和外周阻力下降。

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