分枝杆菌

Methods The total proteins of untreated and INH-treated mc^2 155 cells were separated by ampholyte pH gradient-based two-dimensional gel electrophoresis (2-DE). Tire differential expression proteins were analyzed by using image analysis software. Results Proteins of control group arid INH-treated group contained 159 and 221 protein spots, respectively.

观察不同浓度的异烟肼处理前后耻垢分枝杆菌生长曲线，确定药物处理SM细胞的最佳时间和浓度，并提取在最佳处理时间和浓度条件下的SM细胞总蛋白，采用载体两性电解质pH梯度双向凝胶电泳技术分离异烟肼处理前后耻垢分枝杆菌mc^2 155细胞的总蛋白质，用PDuest 7.3.1图像分析软件比较分析，以识别差异表达的蛋白质。

Paratuberculosis (MAP,Mycobacterium paratuberculosis) induce a series of characteristics as chronic hyperplastic enteritis andprogressing extenuation in ruminants also called Johne\'s disease.

副结核病是由副结核分枝杆菌即禽分枝杆菌副结核亚种Mycobacterium avium subsp。

objective to study the curative effect of fulguration combining mycobacterium injection in treat ment of condyloma acuminatum.methods the patients with ca were treated by fulguration in large range and local mycobacterium injection under fulguration wound.results the recurrence rates were 20.59% and 58.82% respectively in the observation group and in the control group.

目的 探讨电灼联合局部注射草分枝杆菌治疗尖锐湿疣的效果。方法治疗组应用电灼大范围炭化疣体，并在创面即疣体的基底部注射草分枝杆菌。对照组单纯采用高频电灼治疗。结果两组愈后复发率分别为20.59%、58.82%，前者明显较后者低，两组比较差异有非常显著性（ p ＜0.01）。

The genus Mycobacterium consists of nonmotile,nonspore - forming aerobic bacilli,(0.2~0.6) m m ×(1~10) m m in size.

分枝杆菌属是一类细长或稍弯的杆菌，因有分枝生长的趋势而得名。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

The main obstacles to the global control of the disease are emerging multi-drug resistant strains of Mycobacterium tuberculosis and the recalcitrance of persistent infections to treatment with conventional anti-TB drugs.

目前，结核分枝杆菌的多重耐药，以及在抗结核药物作用过程中结核分枝杆菌的持留状态，已成为全世界结核病控制工作的主要障碍。

Objective: To investigate the in-vitro interferon-gammarelease assay based on different Mycobacterium tuberculosis antigens in the diagnosis of tuberculosis and of latent tubercular infections, and to compare it with the tuberculin skin test.

目的 探讨三种结核分枝杆菌抗原刺激外周血单个核细胞后γ干扰素检测对于结核分枝杆菌潜伏感染及结核病的诊断意义，并与结核菌素皮试进行比较。

We observed marophages pre-treated with ConA spread more rapidly on the well bottom compared with PBS injected controls under phase-contrast microscope. SEM of macrophages treated with ConA showed marked larger size and many surface ridges or ruffles and fine fingerlike processes or filopodia in comparison to controls, surface of which were smooth.

结果表明：①ConA处理后，倒置显微镜下可见Mφ粘附性增强；扫描电子显微镜下见其体积增大，指状突起增多；透射电子显微镜下见Mφ内线粒体等细胞器增多，伸出伪足包绕结核分枝杆菌并内吞，ConA活化的Mφ吞噬结核分枝杆菌数量增多，菌体的完整性受损。

In view of these facts, We tried to find something in the L-form induction, plasmid extraction and their function, and the cloning and expression of promotors from BCG in Bacillus subtilis. Our aim is to provide a basis for the expression of HBsAg gene in BCG and to add something to the molecuiar biology of mycobacteria.

正是有鉴于此，我们试图在分枝杆菌在液体培养基中的L型实验诱导、质粒的提取及其功能分析、卡介苗启动子在枯草杆菌的克隆和表达等方面有所发现，为利用卡介苗表达乙型肝炎表面抗原基因提供基础，也为我国分枝杆菌的分子生物学研究增加内容。

Ethambutol is a first-line drug used for antituberculosis therapy. It is often used in combination with isoniazid, rifampin, pyrazinamide, and streptomycin. The action of EMB in antituberculosis mechanism is the destruction of cell wall of tubercle bacilli via binding the target molecule -- arabinosyl transferases. These enzymes are involved in the polymerization of the cell wall arabinan. Inhibition of arabinan synthesis by EMB results in the accumulation of mycolic acids, leading to cell death.The EMB-resistance of MTB is involved with the mutation of emb operon or the over expression of emb operon.

乙胺丁醇是和异烟肼、利福平、链霉素、吡嗪酰胺联合治疗结核病的一线抗结核药物，EMB对结核分枝杆菌的作用机制与分枝杆菌胞壁结构破坏有关，主要作用于靶分子阿拉伯糖基转移酶，抑制了阿拉伯糖基聚合入阿拉伯半乳聚糖，从而影响细胞壁分枝菌酸-阿拉伯半乳聚糖-肽聚糖复合物的形成，导致菌细胞的死亡。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。