分枝杆菌

Objective A prospective study was organized to determine the accuracy and costeffectiveness of the TB AccuProbe DNA hybridization test,the PCR bases LiPA assay,and a verifying automated DNA sequencing of the rpoβ gene,for the routine rapid and direct identification of the Mycobacterium tuberculosis complex and Non Tuberculosis Mycobacterium and rifampin susceptibility.

目的用 3种先进的技术-TBAccuProbeDNA杂交法、INNO LIPATMRIF TB探针试验和rpoβDNA基因序列测定的前瞻性研究，以鉴定结核分枝杆菌复合物和非结核分枝杆菌及其成本效益。

Results There was no hybridizing reaction with other Mycobacterium except human MTB, cow MTB, Microtus and Africa Mycobacterium.

结果 通过PCR联合探针检测结果显示：该探针除了与人型、牛型、田鼠及非洲分枝杆菌有特异杂交信号外，与其他的分枝杆菌没有杂交反应。

Objective To investigate the feasibility of multiplex PCR for rapid identification of Mycobacterium tuberculosis and non-tuberculosis Mycobacteria.

目的 探讨应用多重PCR技术快速鉴定结核分枝杆菌与非结核分枝杆菌的可靠性。

Surgical debridement and anti-Mycobacterium therapy is the treatment of the nontuberculous mycobacteria hand infection in an effective way to.

手术清创和抗分枝杆菌联合治疗是治疗手部非结核分枝杆菌感染的有效途径。

To set up a quantitative suspension mycobactericidal test, and study the efficacy of 6 frequently used disinfectants against mycobacteria.

目的： 1。建立分枝杆菌定量杀灭试验方法，研究常用消毒剂对分枝杆菌的杀灭效果。

Methods From June 2005 to March 2008, treated 20 cases of the nontuberculous mycobacteria hand infection patients after the start of functional rehabilitation treatment time were divided into Group A, B, each of 10 cases, both surgical debridement and anti-Mycobacterium therapy, rewirable after the first day until the wound for wound healing time, follow-up to 6 months after the operation, observe the wound healing time and the recurrence; according to the joint activity score standard score comparison group A, B group of before and after six months of the functional differences.

对2005年6月～2008年3月收治的20例手部非结核分枝杆菌感染患者按术后开始进行功能康复治疗的时间随机分为A组（术后1周）、B组（术后3周），每组10例，均行手术清除病灶和抗分枝杆菌治疗，以术后第一日起至伤口拆线为伤口愈合时间，随访到术后6个月，观察伤口愈合时间、复发情况；根据关节活动度评分标准评分比较A组、B组术前与术后6个月功能的差异。

We concluded that sputum cultures in the Taipei Hospital Branches for Communicable Disease Control revealed that 4.06% were multi-drug resistant strains of Mycobacterium tuberculosis and a very high percentage of multi-drug resistance in Nontuberculous Mycobacterium, which requires the attention of health workers.

研究分析结果显示台北市立联合医院疾病管制院区所培养分离的菌株中结核分枝杆菌多重性抗药菌比例达4.06%，但非典型结核分枝杆菌抗药性之菌株比例却非常的高，值得密切的注意。

Methods Targeting the most common mutation types at codon 526 and codon 531 of Rifampiein Resistance Determining Region of rpoB gene from Mycobacterium tuberculosis ,labeled probes （526CAC, 526TAC, 531TCG and 531TTG） were designed to detect 38 Rifampin-resistant clinical isolates with known RRDR sequence, 24 Rifampin-sensitive clinical isolates with wild type sequence of RRDR and 5 nontuberculous mycobacteria isolates, then a method using real-time PCR was established.

方法针对结核分枝杆菌rpoB基因利福平耐药决定区（Rifampicin Resistance Determining Region，RRDR）526密码子和531密码子常见的突变形式设计探针（526CAC，526TAC，531TCG和531TTG），应用已知rpoB基因RRDR区序列的38株利福平耐药临床分离株和24株利福平敏感临床分离株以及5株非结核分枝杆菌菌株建立荧光定量PCR检测基因突变的方法。

Conclusions］There is a significant difference at protein level between untreated and ethambutol treated mc2155 cells. It suggests that the differential expression analysis of proteomes may be useful for further study on microbiostatic mechanism of ethambutol and search for new targets toward anti-TB drug development.

结论］乙胺丁醇处理前后耻垢分枝杆菌mc2155细胞的蛋白质组具有差异，这种蛋白质组的差异分析有助于进一步研究乙胺丁醇抑制分枝杆菌生长的作用机制并为寻找新的抗结核药物作用靶标奠定基础。

［Objective］To investigate the microbiostatic mechanism of ethambutol, proteomic methods were used to find differential expression proteins in Mycobacterium smegmatis mc2155 cells treated with ethambutol.

［目的］利用蛋白质组学方法，识别乙胺丁醇处理前后耻垢分枝杆菌mc2155细胞后差异表达的蛋白质，为进一步探讨乙胺丁醇抑制分枝杆菌生长的作用机制及寻找新的抗结核药物作用靶标奠定基础。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。