分枝杆菌

Professor Jon Rhodes, from the University's School of Clinical Sciences, explains: Mycobacterium paratuberculosis has been found within Crohn's disease tissue but there has been much controversy concerning its role in the disease.

"乔恩罗德教授，从大学学院的临床科学，解释说："分枝杆菌已发现克罗恩病组织，但已经取得了很大争议，关于它的作用，这种疾病。

The research results and expression product could serve as a basis for further studies on the gene-engineering vaccine, subunit vaccine and nucleonicacid vaccine against Bovine Paratuberculosis.

为进一步研究副结核分枝杆菌基因工程亚单位疫苗、核酸疫苗及诊断试剂奠定了基础。

Paratuberculosis;map0862 gene;map2154c gene;splicing overlapping extension;recombinant expression

禽分枝杆菌副结核亚种；map0862基因；map2154c基因；重叠延伸剪接技术；重组表达

The allergy quarantine with avian bacillustuberculosis PPD indicated that there is significantly difference between the pVAXl-hsp65,pVAX1-hsp65-10 groups and supersonic antigen of MAP group (P＜0.01).This indicates that the Hsp65 andHsp 10 does not interfere with detection of Paratuberculosis allergy.

禽结核杆菌PPD皮试结果显示，pVAX1-hsp65和pVAX1-hsp65-10免疫组与副结核分枝杆菌超声抗原免疫组差异极显著(P＜0.01)，表明Hsp65和Hsp10不干扰变态反应检测。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

In order to develop an indirect ELISA for diagnosing bovine paratuberculosis, the DNA fragmentsof map086 2and map2154c were amplified from the genome DNA Mycobacterium avium Subspeciesparatuberculosis K10. Then the DNA fragments of map0862 and map2154c were spliced byoverlapping extension, yielding a fusion gene map0

为了建立一种有效的牛副结核病间接ELISA方法，本研究设计了两对引物，从副结核分枝杆菌P_(18)株的基因组中PCR扩增出两个目的基因map0862和map2154c、，采用重叠延伸剪接技术(splice by overlapping extension，SOE)获得融合基因map0

Ozdemir HM, Us AK, Ogun T The role of anterior spinal instrumentation and allograft fibula for the treatment of Pott disease［J］ Spine,

8〕周劲松，陈建庭，金大地，等结核分枝杆菌对材料粘附能力的体外实验研究［J］中国脊柱脊髓杂志，2003，

Objective To explore the feasibility of crystalline nitrate reductase reagent used in the drug susceptibility test of Mycobacterium tuberculosis.

目的探讨固体硝酸盐还原酶试剂用于结核分枝杆菌药物敏感性试验的可行性。

In addition, the 3D-model structure of PvFadA is composed of 11 helixes, moreover,α3,α4,α6 and α7 form a core 4-helix bundle to regards as an active center in this enzyme, similar as acyl-ACP desaturase of Ricinus communis and Mycobacterium tuberculosis H37Rv.

PvFadA的3D结构类似于蓖麻和结核分枝杆菌H37Rv的acyl-ACP去饱和酶。

To construct the recombinant plasmid of protein 38kDa of M. tuberculosis and express, purificate and renaturate fusion protein 38kDa and to evaluate its potential value for TB serodiagnosis.

目的 构建结核分枝杆菌38kDa的重组质粒，在大肠杆菌中表达、纯化和复性重组蛋白，并评价其在结核病血清学诊断方面的价值。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。