冰冻切片

Observed the skin 24h,5d and 10d after injection,and maked frozen section from the ultraviolet radiation area of guinea pig skin.Observed migration and ecesis of the BMSCs marked by DAPI in ultraviolet radiation area of guinea pig skin by laser scanning confocal microscope.

注射后24h、5天及10天观察豚鼠皮肤变化，同时取照射区的皮肤制备冰冻切片，激光共聚焦显微镜下观察DAPI标记的BMSCs在紫外线照射区皮肤组织内的迁徙及定居情况。

Rapid immunohistochemical staining by EPOS combined with intraoperative frozen section could reduce the false negative rate of SLN. And those who with nagative SLNs could apply simplified ALND safely, and decrease operation injury and complications.

EPOS法联合冰冻切片可减少SLN的假阴性率，SLN阴性可安全使用简化的ALND，减小手术创伤和并发症。

SLNs of 48 patients with early-staging breast cancer were detected by methylene blue staining SLNB with intraoperative frozen section combined with rapid immunohistochemical staining by EPOS. 28 patients were found negative, and were randomized to receive simplified ALND(n=15, experiment group) or standard ALND(n=13, control group).

30例早期乳腺癌行美蓝染色SLNB术中行SLN冰冻切片联合EPOS法快速免疫组化检查，其中阴性者28例分成两组，15例行简化的ALND(仅清扫胸小肌外侧组淋巴结，简化组)，13例行标准ALND。

The results showed that the relationship of circumferential stress-strain of SMV could be expressed with the exponential equation.

原始管壁厚度的测量，采用冰冻切片苏木精染色后，用测微尺测量8个位点，取均值。

Methods 45 RA patients, 46 patients with other rheumatic diseases and 20 volunteers were enrolled in the study. The frozen sections from human gena mucous and rat esophagus were respectively used as the substrates. The APF, AKA and RF were determined by indirect immunofluorescence.

为45例RA患者，46例非RA的风湿性疾病患者及20例健康人，分别以人颊黏膜和大鼠食管冰冻切片为底物，用间接免疫荧光法检测APF、AKA及RF。

ObJective To investigate the distribution of hematoporphyrin monomethyl ether in rabbit choroidal melanomas by dye fluorescence angiography and fluorescence microscopy.

目的 应用血管造影和组织冰冻切片荧光显微镜观察的方法，研究国产新型光敏剂血啉甲醚（hernatoporphyrin monomethyl ether，HMME）在兔脉络膜黑色素瘤眼部组织的分布。

Orthotopic hybridism in situ: To study the expression in situ of MMP-3 and NF-κB in frozen slices.

原位杂交：观察MMP-3和NF-κB p65在对照组和通心络组冰冻切片中的原位表达。

Abdominal LA is a rare and special condition of lymphatic tissue maldevelopment with diffused involvement.

腹腔脏器LA是一种少见特殊类型的疾病，一经诊断应积极手术治疗，术中冰冻切片、术后病理检查， LA完整切除是关键。

Brains were removed cut on a freezing microtome at a thickness of 30 m. Immunofluorescence technique was used to detect BrdU and Nestin, NSE and GFAP were detected by immunohistochemistry.

取10一巧代的细胞，BrdU标记后在立体定向下通过细针移植入成年鼠的纹状体和大脑皮层，分别于移植后1周和6周取脑行冰冻切片，行BrdU和Nestin免疫荧光检测，以及NSE和GFAP免疫组化染色。

Methods AD model was established by injecting β amyloid protein into the hippocampus of rat. The rats were killed ten days after injection. The hippocampus sections were made coronally on a freezing microtome.

采用海马注射A淀粉蛋白的方法建立AD模型。10d后对大鼠进行灌注，取脑，冰冻切片，用NGF、BNDF和NT3抗体行免疫组织化学染色。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。