冰冻切片

To characterize the isotype of human xenoreactive nat ural antibodies against porcine pancrease islet cells, cyostat sections of the p ancrease were incubated with the sera from 18 cases of IDDM or 20 human controls respectively, further with FITC-conjugated goat antihuman IgG or IgM secondary antibodies. IgG was found to bind to pancrease islet cells in sera from 8 cases of IDDM and 8 human controls, while IgM in 4 IDDM and 6 human controls.

为检测人抗猪胰岛细胞天然抗体的同种型，采取18份胰岛素依赖型糖尿病患者血清及20份正常人血清分别与杂种猪胰腺组织的冰冻切片孵化后，再用免疫荧光标记的羊抗人IgG、IgM单克隆抗体染色。8份IDDM血清和8份正常人血清中含有直接针对猪胰岛细胞的IgG型抗体；4份IDDM血清和6份正常人血清中含有IgM 型抗猪胰岛细胞抗体。

Nerve repair using freezing and fibrin glue: immediate histological improvement of axonal coaptation.

应用"细胞外科"技术进行神经修复后的早期冰冻切片观察。

Three gastric noncancerous tissues were cryo-sectioned, stained with Cresyl Violet, and pathologically rechecked. Epithelial cells were obtained by LCM and RNA was isolated. Agilent 2100 bioanalyzer was used to check the RNA quality.

选取3个低温保存的胃癌旁组织样本，冰冻切片进行甲酚紫染色和病理学检查，利用激光显微切割技术分离非癌上皮细胞，提取RNA并以Agilent 2100生物分析仪鉴定RNA的纯度和完整性。

The inner earspecimens From E8 to Da of C57BL/6 mouse were made into cryostat sections.

对E8-Da的C57BL／6小鼠的内耳发育时间段的内耳标本进行冰冻切片处理。

The results indicatedthat the area and perimeter of nucleis on frozen slices were much larger than those on paraffined slices.

结果发现冰冻切片时核面积及周长均较常规切片时显著增加，而形状因子无明显差异。

Three parameters (the area, perimeter,form factor of nucleis)were measured on frozen and paraffined slices respectively and comparatively.

本文采用电子计算机图象分析技术对6种16例常见肿瘤细胞核的周长、面积、形状因子3组参数，进行了冰冻切片及相应石蜡切片的对比检测。

All experiment animals lived well with no death or malignant arrhythmia following cell transplantation. Frozen sections of the heart showed a few blue-stained cells under the fluoroscope in the cell transplantation group 4 weeks later. This indicated that stem cells were successfully homing. Using hematoxylin-eosin staining, homing cells were distributed over the infarct zone, with the presence of single or mass. In the model control group, no blue-stained cells were determined.

细胞移植后两组大鼠均存活良好，未出现死亡及恶性心律失常，4周后细胞移植组心脏冰冻切片荧光镜检显示心肌内有少量胞核蓝染的细胞，提示干细胞成功归巢，结合苏木精-伊红染色，归巢细胞位于心肌梗死灶周边区域，呈单个或小团聚集；而模型对照组心肌切片未见胞核蓝染的细胞。

Methods We established the model of human hair follicle in vitro in Williams E serum-free medium. We can continuously observe morphologic change of hair follicle under an invert microscope. The length of hair follicle was measured by eyepiece micrometer and DNA synthesis rate of human hair follicle in vitro by measuring the rates of incorporation of 3H-TdR.Histology form was observed by Cryo-section and transmission electron microscope.

在Williams E无血清培养基中，建立离体人头皮毛囊培养模型；通过倒置显微镜观察毛囊的形态变化；目镜测微器测量毛囊长度；3H-TdR 掺入检测毛囊DNA合成率；组织学检测用冰冻切片光镜观察和电镜切片透射电镜观察。

Pulp reactions were obvious during 3 days after lased. Alkaline phosphatase examination: After fixed, decalcified and selected, all experimented teeth were stained with the Ca-Co method in order to examine the alkaline phosphatase activity in dental pulp cells.

碱性磷酸酶的检测：实验牙在丙酮中固定1 d，再置入复合酸中脱钙，冰冻切片后，进行钙-钴法染色以显示牙髓细胞中碱性磷酸酶活性，根据米德冷图象分析系统测定切片的灰度值，进行统计学分析。

Classify 31 patients with leukorrhea diseases of CIN into 4 syndromes: syndrome of spleen asthenia, syndrome of kidney asthenia, syndrome of dampheat and syndrome of damptoxin.

结果：60例CIN和炎症患者，宫颈鳞状上皮苏木素染色后直接冰冻切片与HE石蜡切片病理结果对比，两者无统计学差异(p=0.833)，说明苏木素染色后苏木素富染区能预知病理结果。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。