伪狂犬病

Key words: pseudorabies virus;gC gene;PCR;biological characteristic

伪狂犬病病毒；gC基因；PCR；生物学特性

Methods Sindbis virus, Pseudorabies virus and poliovirusl (PV1) were used as indicated viruses in this test.

将Sindbis病毒、伪狂犬病毒和脊髓灰质炎病毒(PV1)3种指示病毒分别加入不同的UTI原料样品中，进行60℃水浴10h和乙醇处理3h，处理不同时间后分别取样，用微量细胞病变法检测不同时间段样品中的病毒残留滴度。

this article be main to used for the chicken non- typical model epidemic disease in Hsin-cheng, the contagion method surname bag, geniality flue, contagion bronchitis, contagion enterogastrtis, breed with breath system comprehensive disease, small virus, foot-and-mouth disease virus, false rabies;The kidney disease of the marine products animal fish disease, the issue of blood openings disease, crazy visit a disease, the terrapin issue of blood bowel way be bad dead, turtle mumps, grass carp bleed far-gone virus the Wen be febrile to prevent°from.

本品主要用于鸡非典型新城疫，传染性法氏囊，温和性流行性感冒，传染性支气管炎，传染性胃肠炎，繁殖与呼吸系统综合症，细小病毒，口蹄疫病毒，伪狂犬病；水产动物鱼病肾脏病，出血性开口病，狂游病，鳖出血性肠道坏死，甲鱼腮腺炎，草鱼出血病等病毒性瘟热病的防止。

When a car is inadvertently overfilred and the tank becomes hydrostatically full, internal pressure can increase asymptotically from even small temperature increases or Balanced Bellows Safety Relief Valves product addition.

当一辆汽车是无意中overfilred和坦克变成静水充分，内部压力增加，即使是小规模渐进的温度升高或产品增加。伪狂犬病毒引起过多的产品驱动放电，它可以帮助防止罐车由于压壳灾难性的失败。 PRVs也有助于防止无意。。。

Attempts to reduce the economic losseshave also included passive immunization with hyperimmune serum and monoclonal antibodies.

为尽可能减少经济损失，人们也尝试了以抗血清、单克隆抗体对伪狂犬病的被动免疫预防与治疗，并取得一定效果。

The eukaryotic expression plasmids were used as genetic vaccines to immunized intramuscularly experimental animals and post-weaning piglets.

同时还研究证明猪白细胞介素2和6基因对猪伪狂犬病核酸疫苗的免疫增效作用。

Through DOT-blot hybridiztion the result indicated SA215 and SA215 were of the same distribution on animal ,simply planting the following trigeminal ganglion .21-day-old SPF pigs inoculated intramuscularly SA215 strain could be protected from being affected at day 28 after oronasal challenge-exposure with 106 PF.U high-dose virulent PRV Fa strain and at day 42 after i.m.

SAZ巧株lml接种21日龄健康仔猪(伪狂犬病和猪瘟检测阴性)，在接种后28天用10毕FU的PRV Fa强毒株进行滴鼻攻毒，42天用猪瘟强毒SM株血毒lml肌肉注射攻击，结果表明接种猪能抵御两次病毒攻击，而同条件下对照结果成立，表明SAZ 15株具有良好免疫原性。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

There was no accumulative effect on immune protection in groups with mix plasmids.

这为猪伪狂犬病核酸免疫研制与应用提供了重要的实验依据。

Methods Aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with pH 4, human immunoglobulin and human hepatitis B immunoglobulin for intravenous injection with pH 4/pasteurization,treatment on human fibrinogen and human coagulation factor Ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.

系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒，人血白作者：魏宪义，魏红，蹇远勇，陈海，刘文芳目的分析血液制品特定灭活因子的时效动力学曲线，科学性地评价与改进不合理的病毒灭活参数。

I'm strongly against the death penalty — it's an eye for an eye.

我不赞成死刑——这是以牙还牙的报复行为。

And to get you the support you need, we're enlisting all elements of our national power: our diplomacy and development, our economic might and our moral suasion, so that you and the rest of our military do not bear the burden of our security alone.

并给你们所须的支援，我们正徵召国家所有各种的力量：我们的外交及发展，我们的经济力量与道德劝说，所以你们与其他军人不须要孤独地负起国家安全的责任。