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Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

This algorithm constructs a 64-D crossband vector according to the characteristics of the decomposed image, and adopts nonlinear interpolative vector quantization and soft computing based codebook generation algorithm. Hence, this algorithm can improve the vector quantization performance significantly from both aspects of speed and precision, and it can get a high reconstruction quality (PSNR＞34dB) with a very low bit rate (0.172bpp).

该方案依据图象小波变换的特点构造了64维的跨频带矢量，并采用了非线性插补矢量量化和柔性计算码书生成算法，从速度和精度两方面提高矢量量化的性能，从而在极低比特率的条件下(0.172bpp)获得了高质量的重构图象(PSNR＞34dB)。

The three conserved histidine residues with a conserved glutamate residue fits the active site of the homology model of oxalate oxidase.2. Construction of plant binary expression vector of germin geneThe germin gene fragment cut with BamH I from intermediated vector pDG28 was inserted into binary vector pBI121 cut with the same enzyme to formrecombinants which was selected by plaque PCR .

Germin基因植物表达载体的构建将中间载体上的germin基因片段用Bam H I酶切下来，插入用同样酶切的双元表达载体pBI121上，以菌落PCR筛选重组子。

This method uses what Professor Salton has proposed as the vector spacial model to express text as an invisible vector in hyperspherical space.It makes use of certain pre-disposal methods (reduce the dimensions,clustering thesaurus,filtering out old-fashioned words) to reduce the dimensions.It usescosines to measure the degree of similaritybetween texts and uses the total similarity as the target convergence vector.It produces clustered results by using restricted iteratives to achieve partial perfection.

该算法用Salton教授提出的向量空间模型把文本表示成不可视的超球向量空间的一个向量，采用一定的预处理手段(降维、同义词归并、停用词过滤)完成维数约减，使用余弦相似度度量文本之间的相似度，并选择总凝聚度作为目标收敛函数，通过有限次的迭代达到局部最优，输出聚类结果。

Our research utilized two cell lines carrying a bisatellite microchromosome originated from two normal phenotypic families found by Professor Jiahui Xia to make the BM become a new vector for gene therapy. The research will finally establish a novel vector that can be steadily transmitted, has enough capacity to carry foreign genes, has no harm to human being, has no immunogenecity and is easy to transfer. This research can provide brand new vector tool for gene therapy of human hereditary diseases and tumors.

本研究利用夏家辉教授发现的2个带有双随体小染色体而表型正常的家系所建立的细胞株作为研究材料，用BM作为基因治疗新载体，以求最终建立一种能稳定遗传、携带外源基因容量足够大、对人体无害、无免疫原性和易于基因转移的新型载体，为人类遗传病和肿瘤的基因治疗提供崭新的载体工具。

When the voltage space vector is located within overmodulation mode I, the locus in the hexagon remain the circular, but the amplitude is larger than the modulus of voltage space vector to compensate the voltage distortion on the edge of hexagon; the reference voltage vector tracks the hexagon sides in the remaining.

实验结果表明，该算法简单易实现，能够有效的提高直流母线的电压利用率，具有在低频运行稳定，逆变器输出电流正弦度好等优点。

To develop an iphRNA vector for seed-specffic expression targeting the fad2 gene in Brassica napus L., an inverted repeated unit of the 537bp segment of the fad2 gene was first cloned into a medium vector, the pllurrican Vector, with a spliceable fad2 intron sequence in between.

通过PCR扩增分离到甘蓝型油菜种子特异性表达的NaPin启动子序列(11147bp)以及油酸脱饱和酶基因(fad2)的一个537bp的片段，并将它们分别克隆到pGEM-T easy载体中。

It is concluded that AD5/F35 vector infection has difinite selectivity to hematologic malignant cells of varions origin,the infection ability of AD5/F35 vector to cells of myeloid origin is stronger than that to cells of B cell origin,the cytotoxicity of AD5/F35 vector to infective targer cells is small.

AD5/F35载体对不同来源的血液恶性细胞的感染具有一定的选择性，对髓系来源细胞感染能力强，对B系来源细胞有一定感染能力，而且对感染靶细胞毒性小。

A highly conserved sequence of 210 bp of AtNHX1 gene that encodes a Na+/H+ antiport protein was isolated from A.thaliana,the 210 bp fragment was amplified as RNAi target site and used in construction of RNAi vector,and was inserted into pHANNIBAL as reverted repeat with an intron.The expressional box was linked into the vector of pART27,NPTⅡgene is the screening marker in this vector.

利用RNAi研究植物基因功能，可选择靶基因的一段单一序列使基因家族中的单个成员沉默；也可以选择某一基因家族的多个成员具有的一段同源性很高的保守序列，设计针对这一序列的dsRNA表达载体使多个基因同时沉默，RNAi成为功能基因组学研究的重要手段。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。