查询词典 vector basis

With the method of reverse transcription-polymerase chain reaction and nest PCR, P80 gene of CSFV is cloned from the cells infected by the strain Shimen of csfv, and connected with eukaryotic expression vector pEGFP-C1. Then recombined eukaryotic expression plasmid pEGFP-P80 of P80 gene is achieved successfully. It builds the basis for expressing and gaining protein p80 in mammiferous cells.

利用反转录PCR和套式PCR技术，从猪瘟病毒Shimen株细胞毒中克隆出P80 基因，并进一步把P80 基因连接在真核表达载体pEGFP-C1 上，成功地构建出P80 基因重组真核表达质粒(pEGFP-P80)，为在哺乳动物细胞中表达并纯化出p80 蛋白奠定了基础。

Malayi to niches in its human and vector hosts and insights into the molecular basis of a mutualistic relationship with its Wolbachia endosymbiont.

负责这项研究计画的美国匹兹堡大学医学院盖汀表示，确定是马来丝虫基因组后，科学家将更快找出更有效的治疗方法。

Objective: On the basis of the random mutagenesis library of ARS, we wish to preparation by screening new ARS sequence which not only have higher ARS activity, but also display higher transformation frequency, in order to constructed new gene recombination vector which have higher efficiency in the Nonconventional yeast expression system.

目的：通过构建酵母ARS 随机突变体库，从中筛选出具有较强的转化效率的ARS序列，用以构建新型高效的克鲁维酵母基因工程载体。

In this package, the operations and functions of vector, matrix and pronumber are implemented by object-oriented programming technique, so that the gradient and Hessian of objective function can be calculated automatically and up to the machine precision of computers, and it provides the basis for accurate and effective optimization.

用面向对象技术实现了向量、矩阵和延拓数的相关运算和函数，使得目标函数的梯度和赫斯矩阵可以自动地求出，并且可达机器精度，为准确而有效的优化算法提供了基础。

A RU486 regulatable replication-defective recombinant adenoviruses vector containing single chain fusion murine IL-12 gene was successfully constructed,which provides a good basis for further research on gene therapy in vivo.

成功构建了重组腺病毒 Ad-RUmIL-12，可以通过RU486诱导mIL-12单链融合蛋白的表达，为下一步体内基因治疗奠定了良好的基础。

Barley yellow dwarf virus is reported to be the most important viral pathogen of cereals worldwide. In fact, BYDV is a group of at least five luteoviruses that cause similar symptoms in cereals but can be distinguished serologically and on the basis of vector specificity.

禾谷类作物的黄矮病是由大麦黄矮病毒(Barley yellow dwarf virus，简称BYDV)的侵染所引起的，在世界各国均有发生，由蚜虫进行循环性的永久传播。

The FEM, whose process is very *** and is fit to deal with complex boundary condition, is used to simulate the frequency-domain IP. Studying the characters and laws of IP\'parameters under different measuring devices and undulate topography will provide scientific references and basis and reduce the multiplicities of interpretation. It is will be an attempt and exploration.Because of the frequency of the exciting currents is usually between 10-2～102 Hz, the vector A can be seen is not change with the time and the EM effect can be ignored in the low frequency.

双频以及伪随机三频激电法以它特有的轻便、快捷、高效率等特点在有色金属矿躲勘探领域里获得了广泛的应用，运用有限单元法的推导过程简单、适合处理复杂几何外形等特点来对双频以及伪随机三频激电法进行正演数值模拟，分析和研究了激电参数在不同丈量装置、模型参数和起伏地形情况下的特点和规律，为后续的资料解释提供科学的依据和参考，为减少地球物理中的多解性做一些有益的探索和尝试。

On the basis of the equivalent mechanism of swinging movable teethed transmission-crank-rocker mechanism, the tooth profile equation of central gear was derived by the use of complex numbered vector method.

基于摆动活齿传动的等效机构－曲柄摇杆机构，利用复数矢量法，导出了中心轮的齿廓方程。

To further elucidate the mechanism of BMP in regulating cell differentiation and to provide basis for BMP gene therapy, this study centers on the effects of BMP2 gene transfection on fibroblasts and contains the following works:① A 1.2kb fragment containing full length human BMP2 coding region was inserted into pBK-CMV (expressed both in protocaryon and eukaryon) by using predetermined orientation methods, resulting in phagemid expression vector for BMP2 (pBK-B2).② pBK-B2 was transfected into NIH3T3 cells by using LipofectAMINE.

为了进一步从分子水平探讨BMP对细胞分化的调控作用，并为BMP基因疗法的建立提供依据，本实验围绕BMP2基因转染对成纤维细胞的生物学作用进行了以下几个方面的工作：①将含有人BMP2全长编码序列的基因片段(1.2kb)定向克隆于噬菌粒表达载体pBK-CMV中，成功构建了人BMP2噬菌粒表达载体pBK-B2。

The finite element method has been used to analyze and calculate the space magnetic fields of wafer magnets and square magnets as well as sphere magnets. Vector magnetic potential, magnetic induction, magnetic force lines and magnetic induction isolines are obtained which provide theory basis for the application of magnetic field.

采用有限元法对磁疗中常用的圆片磁源、方片磁源、球形磁源的空间磁场进行了分析和计算，得到了上述磁源空间磁场的矢量磁位、磁感强度、磁力线和磁感强度等值线等参数，为磁源的使用提供了理论依据。

What was it that made you want to change sider ?

是什么使你临阵倒戈？

I don't know what he wants, but let's play along with him.

我不知道他要干什么，可我