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In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

Even until advanced age about 70 years old she still comes out again to see a matter,.Chanel group is in 1983 served as a vogue director general by Karl Lagerfeld, but the up to now each quarterly new product still take Chanel spirit as in order to design a principle, Chanel, lasting Chanel.Quote from a words in Chanel:"I not apprehensibility woman why can't just for the sake of the performance manner, before go out all dress up well once, every day who know to can be to in the clout a predestined big day?"

甚至一直到70多岁的高龄她都还复出视事，香奈儿集团在1983年由Karl Lagerfeld出任时尚总监，但至今每一季新品仍以香奈儿精神为为设计理念，香奈儿，永远的香奈儿引用香奈儿一句话："我不能理解女人为何不能只是为了表现礼貌，出门前都好好打扮一下，每一天谁知道会不会是命中注定的大日子？"

Mankind's history up to now helps a culture by torture Jun method to often see not fresh, like the Xing of fire Fen, commit by poison of Xing, behead, cut a waist, bury alive, boil Lao, car crack, Ling2 Chi2, disorderly the stick kill, execute by shooting, exterminate whole family, five horse cent corpses, dig to the prisoner's large intestine to bind while also having Sung Dynasty Bad is at the horsetail up, whip son a ring the horse drive fast and the prisoner immediately draws last breath an etc.

人类历史至今以酷刑峻法协助教化者屡见不鲜，如火焚之刑、服毒之刑、斩首、斩腰、活埋、烹烙、车裂、凌迟、乱棍打死、枪决、灭门、五马分尸，还有宋朝时把犯人的大肠挖出绑在马尾巴上，鞭子一响马飞驰而去，犯人立即气绝等等。

Shanghai Fufeng labor tradz industrial CO.,LTD will be a have many a year mold development,Manufactures together 's enterprise,Company in recent years and increases trade department,And set up have lapin purchase at Anhwei Fuyang area, There is dress trade department at Jiangsu changshu enactment ;Our company a section develop design, Manufacture sale, Serves trade together 's large-scale comprehensive enterprise,Company since put up a factories develop up to now, Many year be like a day, Consideration quality is like life, As good faith win market, Down-to-earth, With steady steps realistic, Manufactures high accuracy quality's mold, And " Appearance modelling beauty, Product quality high "'s sports appliance, But long minus great reputation.

上海阜峰工贸有限公司是一家有多年模具开发，生产于一体的企业，公司近年来又增加了贸易部，并在安徽省阜阳地区设置有兔毛收购点，在江苏省常熟市设置有服装批发部；本公司集研发设计、生产销售、服务贸易于一体的大型综合性的企业。公司自建厂发展至今，多年如一日，视质量如生命，以诚信赢得市场，脚踏实地，稳步求实，所生产高精度品质的模具，和"外观造型美，产品质量高"的体育用品，而久负盛誉。

IE is released can use in Windows system only all the time up to now, but look now, no matter be to average consumer or enterprise user character, microsoft wants have the aid of this browser retains the user the think of a way in Windows system already malapropos.

IE发布至今一直都只能在Windows系统中使用，但现在看来，无论是对普通消费者还是企业用户而言，微软想要借助这款浏览器将用户保留在Windows系统中的想法已经不合时宜。

Look be like all using up to be in people is expected, however, the person of history of course of study of act on one's behalf of twilight China goods knows, walk out of the carry outside the China that come from inside harships course of 50 years, still can shed industry group stride to the contemporary and integrated content of modern, internationalization with steady pace up to now, still can walk to shed the market at domestic and international content with the figure with bright, abundant vigor, its successful backside, have logic one time oneself.

看似一切尽在人们意料之中，然而，略知中国货代行业历史的人知道，从50年的风雨历程中走出来的中国外运，至今仍能以稳健的步伐向现代化、国际化的现代综合物流企业集团迈进，仍然能够以鲜亮、活力充沛的形象行走于国内外物流市场，其成功的背后，是自有一番逻辑的。

Teleview drama is video website most the user behavior habit of the mainstream, actor cruel from 2008 bottom up to now, its " of significant progress strategy adds up to the working focus that plans " to be put to the plan and construction of teleplay theater mode, such as heat sows theater, Han drama theater, TVB theater, a tough guy the many themes theater such as the theater, cater to close differently inspect be fond of, the lock decides different user crowd. March, actor cruel with the war epic gigantic make " my colonel my group " head hair roll out heat to sow a theater, from the warm-up to this drama conduct propaganda arrives broadcast the strong complement that all becomes TV, earn the eyeball of countless netizens, be less than the time of half month, had achieved day of UV200 10 thousand, thin volume is average VV is close the discharge dimensions of 1 million.

收看电视剧是视频网站最主流的用户行为习惯，优酷自2008年底至今，将其重要发展战略"合计划"的工作重心放到电视剧剧场模式的规划建设上，诸如热播剧场、韩剧剧场、TVB剧场、硬汉剧场等多个主题剧场，迎合不同的收视喜好，锁定不同的用户人群。3月份，优酷以战争史诗巨制《我的团长我的团》首发推出热播剧场，从对该剧的预热宣传到播出均成为电视的有力补充，赚得了无数网民的眼球，不到半个月的时间，已经达到日UV200万、单集平均VV近百万的流量规模。

In view of this point, Lu Ling's novels are of value, because strong intellectual complex is embedded in his novels describing either workers or peasants, in which the character's spiritual dynamics receives utmost concernViewing from the perspective of the intellectual quality-freedom, enlightenment and reflection, this dissertation seizes up the traits of intellectual character of Lu Ling's novels in 1940's, in an attempt to reproduce the sheen of his novels, and also to take a glimpse at the psychological journeys of the youth generation at that ageThose characters in Lu Ling's novels behave unbridledly all the way through their life journeyo They rebel against all the absurd things, declare war on tradition, family and newly rising authority, also anatomize themselves They resort nowhere Their life is closely linked to tramp It is right during the course of tramp and rebellion that they realize themselves, look for freedom and luminosity, and achieve real contact with people; they have enjoyed the unrestraint to their heart's content as well as experience torture from various forces in heartLu Ling's novels are affluent in connotation This dissertation only studies from the perspective of intellectual, the topics among which like: freedom, tradition, individual and collective are still worthwhile studying up to now.

从这一点出发，路翎的小说很有价值。他的小说不管是描写工人还是农民，都带有浓重的知识分子情结。他的关于知识分子的小说，更是极力关注人物的精神动态。本文从现代知识分子品质的角度——自由、启蒙与反思，打量路翎四十年代小说中的知识分子人物的特质，企图在这种论说中使路翎小说的光彩重现，也从中窥视那一代青年在那个时代的心路历程。他小说中的那些人物，在人生的道路上一路狂飙过去。他们反叛一切不合理的东西，对传统、家庭、新生的权威开战，也对自己身上旧的、暧昧的因子毫不留情。他们在哪里也停留不下，他们的人生和流浪紧密地联系在一起。在这个不断的流浪和叛离中，他们认识自己，追寻自由、光明，力求与人民的真正接触。也正是在这个背叛—寻找的过程中，他们享受了狂飙的酣畅淋漓，也体会到了内心各种力量撕裂的痛苦。路翎小说的内涵很丰富，本文只是抽取了知识分子这个角度进行研究。当中的一些命题：自由、传统、个人与集体在今天还是很有研究价值的。

Ex : j Until now, I have not seen any audience.k Up to now, I have not been able to forget how nervous I wasat that time.

until now / up to now是表示从过去到现在的一段时间，因此要用「现在完成式」来表达。

During Catholicism was spreaded in Shanxi the roles that the foreign missionaries,Chinese clergies and its believers played were important parts of Shanxi Catholic history.They respectively played important roles during the Catholicism incultured in Shanxi.Up to now,the behef of the Catholics,their rituals and their basic conditions such as their age,sex,status and economic situation were shortages of Chinese Catholic research field.Studying the policies and attitudes that Shanxi local officials towards the Catholicism and the relationship between the believers and non-believers can provide experiences and lessons for Shanxi temporary government and people,now they are building up harmonious society,these experiences and lessons can help them treat the Catholics properly.

天主教在山西传播过程中外国传教士、国籍神父及本地教徒各自充当的角色、发挥的作用是山西天主教史重要的组成部分，他们分别为天主教在山西的本土化发挥了各自的作用；教徒的信仰内容、各种礼仪以及身份、年龄、性别、经济状况等基本情况是目前为止中国天主教史研究领域的短缺部分；对自天主教传入山西以来山西地方官对天主教的政策、态度以及民教关系的研究，则能于当前构建和谐社会的过程中，为政府、民众正确对待山西天主教徒，处理好和他们的关系，提供历史经验借鉴。

Methods: Five patients with parkinsonism or dystonia were assigned to general anesthesia using an modified endotracheal tube.

本实验依照人体实验之相关规定进行，五位患有帕金森氏症或肌张力异常的病人接受神经立体定位手术。

If you can benefit from this book, it is our honour.

如果您能从本书获益，这将是我们的荣幸。