查询词典 tumor

Under inhibit tumor fall increment andalert confession The objective of this experiment is to prove the antitumor effect and its mechanism in vivo of Erysimum cheiranthoides. In this study we observe the ratio of inhibitory of mice bearing S180 and the effect of prolonging lives of mice bearing H22;the effect on immune function of mice bearing S180;the phagocytosis of MO cells; measuring serum TNF of mice bearing S180 with ELISA; the expression level of p53 in tumor tissue with ICH and the change of tumor cells shape with pathology test.

实验采用S_(180)、H_(22)两种瘤株，以昆明利，小鼠为研究对象，对体内的抑瘤作用、生命延长作用进行研究；对S_(180)荷瘤小鼠免疫功能影响的研究；采用碳粒廓清法研究该药对巨噬细胞吞噬功能影响的研究；采用ELISA法检测S_(180)荷瘤小鼠外周血TNF含量；采用免疫组化法测定S_(180)小鼠瘤组织中p53蛋白的含量并观察瘤细胞的病理形态。

Methods Nuclear β-catenin expression was detected by immunohistochemistry in 77 lesions with desmoid-type fibromatosis and 171 other spindle cell lesions, including superficial fibrmoatosis (n=18), nodular fasciitis (n=36), keloid (n=16), scar (n=10), granulation tissue (n=9), synovial sarcoma (n=38), neufibroma (n=13), solitary fibrous tumor (n=12), gastrointestinal stromal tumor (n=10), low-grade myxofibrosarcoma (n=3), low-grade fibromyxoid sarcoma (n=3), and smooth muscle tumor (n=10). In addition, the immunohistochemical expressions of ER-α, ER-β and Ki-67 were examined in all of the lesions with desmoid-type fibromatosis. The nuclear immunohistochemical staining for nuclear βcatenin and ER-β was graded as high level (≥25% of cells), low level (5%-25%) or none.

采用免疫组织化学染色法检测77例韧带样型纤维瘤病和171例其它良、恶性梭形细胞病变（包括结节性筋膜炎36例、浅部纤维瘤病11例、瘢痕疙瘩16例、增生性瘢痕10例、肉芽组织9例、滑膜肉瘤38例、神经纤维瘤13例、孤立性纤维性肿瘤12例、胃肠间质肿瘤10例、低度恶性黏液纤维肉瘤3例、低度恶性纤维黏液样肉瘤3例及平滑肌肿瘤10例）组织中β-catenin核阳性的表达，同时检测韧带样型纤维瘤病中ER-α、ER-β和Ki-67的表达。

Result: Among 48 cases, 35 patients (72.9%) had tumor,which was in the top causes for bloody ascites, in which 18 cases were gastroenterological tumor, while 13 patients had not malignant tumor (27.1%).

结果：病因为恶性肿瘤35例，居首位，占72.9％，以消化道肿瘤占大多数，其中癌性腹水22例。病因为非恶性肿瘤13例，占27.1％，病因较多样化，呈分散。

Result: Among 48 cases, 35 patients (72.9%) had tumor,which was in the top causes for bloody ascites, in which 18 cases were gastroenterological tumor, while 13 patients had not malignant tumor (27.1%).

对该病的病因，临床表现特点，实验室及影像学综合分析。结果：病因为恶性肿瘤35例，居首位，占72.9％，以消化道肿瘤占大多数，其中癌性腹水22例。

The efficacy of tumor vaccine is mainly affected by both heterogenicity of tumor cells and complexity of tumor antigens.

另 外肿瘤疫苗的效价也会受到肿瘤抗原成分的异质性及多样性所影响。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Several anti-tumor cell lines and carbonic anhydrase Ⅱ model were established. More than 10000 compounds and extracts from plants were screened using these models and about 10 compounds showed strong inhibitory activities. Two species of plants Inula helianthus-aquatica and Saussurea deltoidea were chosen to investigate chemical and bioactivity constituents based on the results of screening of extracts and fractions. 58 compounds were isolated and identified from these two plants, 6 compounds were found to be new and 14 compounds showed anti-tumor activities. We also studied the anti-tumor mechanism of the compound bigelovin isolated from Inula helianthus-aquatica. Bigelovin was found with apoptotic activity against cell line U266 by lowering the amount of important transcription factor E2F-1 which inhibits the cell cycle at G0/G1 stage. Docking study on 7 natural new phenolic inhibitors of CA Ⅱ and 3D-QSAR study on 61 sulfonamide inhibitors of CA Ⅱwere also investigated. The calculated results were in good correlation with experimental results.

基于以上两种重要疾病和研究组方向，本论文工作开展了多个肿瘤细胞株和碳酸酐酶Ⅱ靶标模型的建立和筛选工作，累计筛选1万多个/次样品，发现10余个活性较强化合物；从粗提取物筛选结果中选取两种抗肿瘤活性强的菊科植物水朝阳旋覆花(Inula helianthus-aquatica)和三角叶凤毛菊进行了系统化学和活性成分研究，分离鉴定了58个化合物，发现6个新化合物和14个抗肿瘤活性化合物；通过合作对水朝阳旋覆花中活性较强的bigelovin进行了抗肿瘤作用机制研究，发现它可能是通过降低G2期重要的转录因子E2F-1，致使细胞周期阻滞在G0/G1期，引起多发性骨髓瘤U266细胞凋亡；开展了7个新的天然酚类CAⅡ抑制剂的对接研究和61个合成的系列磺酰胺类CAⅡ抑制剂的3D-QSAR分析，结果表明计算与实验一致。

CCSK is a rare type of renal neoplasm in childhood and its diagnosis relies mainly on histopathology and immunohistochemistry. It is important to be familiar with the morphological variants of CCSK to avoid confusion with other similar lesions, such as Wilm's tumor, congenital mesoblastic nephroma, malignant rhabdoid tumor of kidney, primitive neuroectodermal tumor.

CCSK是一种罕见的儿童期恶性肾肿瘤，诊断主要依靠组织病理学和免疫组化，熟悉其形态学变异有利于与其它类似病变如肾母细胞瘤、先天性中胚叶肾瘤、肾恶性横纹肌样瘤、原始神经外胚叶肿瘤等鉴别。

Metanephric adenoma is a very rare benign renal tumor with an embryonic architecture and primitive cytological appearance, superficially mimicking Wilms' tumor. This tumor sometimes reaches a considerable size (20cm) and still runs a benign clinical course.

后肾腺瘤是一个非常罕见的良性肾脏肿瘤，它表现出类似胚胎的结构并且具有原始的细胞外观，有时会形成一个大於20公分的大型肿瘤。

The features of ultrasound image of adrenal cortex carcinoma were 1The tumor was big,mainly isoechogenic,more than 8.2cm in diameter;2The tumor shape was varied,irreqular or multilobar;3Color Doppler showed plenty blood supply of the tumor and its surroundings and 4Tumor necrosis frequently seen.

1。肾上腺皮质腺癌的声像图特征为：①肿瘤是以等回声为主的较大肿瘤，本组均大于8.2cm；②肿瘤形态多样，以不规则形及分叶状常见；③彩色多普勒血流显示瘤内及瘤周血流丰富；④肿瘤内多见液化坏死。2。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。