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Second, residues in the reactive site of the inhibitor were replaced by the consensus substrate recognition sequence of furin, namely, Arg at P_1, Lys at P_2, Arg at P_4 and Arg at P_6. In addition, the P_7 residue Asp was substituted with Ala to avoid possible electrostatic interference with furin inhibition. Finally, the extra N and C terminal residues beyond the doubly conjugated disulfide loops were further truncated. However, all resultant synthetic peptides were found to be temporary inhibitors of furin and kexin during a prolonged incubation, with the scissile peptide bond between P1 and P'1 cleaved to different extents by the enzymes.

高活性、低分子量的furin酶抑制剂有可能发展成为新型的抗细菌内毒素和抗病毒感染的药物,我们以绿豆胰蛋白酶抑制剂Lys片段长链22肽为模板,设计了一系列能抑制furin和kexin活性的突变体,采用如下三种方法逐步优化:一、去除Lys片段长链三对二硫键中的一对,用Ser替换Cys,减少可能存在的二硫键错误配对;二、抑制剂的活性中心根据furin底物的专一性逐步改变,即P1、P2、P4和P6位分别变为Arg、Lys、Arg和Arg,同时为了避免P7 Asp和P6 Arg之间可能存在的相互作用,将P7位Asp替换为Ala;三、将两对二硫键形成的双环的外面5个氨基酸残基去除。

The statement 'if there were not in existence something other than sensa, we should not be able to do what we are able to do (viz., name things)' can perhaps be seen as offering a truncated argument.

如果感觉之外别无他物,我们将不能做我们现在能做的事情,即命名。这个论证或许可以看做是一个删节版。

A dominating antigenic region located at the amino-terminus of Hantaan virus nucleocapsid protein.These recombinant truncated proteins can be used to diagnose the infection of HFRSV serologically.

汉滩病毒核蛋白的氨基端含有一主要抗原决定簇区;原核表达的截短核蛋白在HFRS的血清学诊断中具有一定的价值。

Three expression vectors containing mutant gfp which were truncated from 3′ termine were constructed in the present paper.The mutation gfp with different length under the control by T 7 promoter were expressed in E. coli BL21 cells.

本文将突变型 gfp3′末端不同程度地截短,构建成表达载体,在大肠杆菌 BL 2 1中表达了不同长度的绿色荧光蛋白,当其生色基因中 65位 Ser点突变成 Thr,则 GFP的激发波由 396nm增至 4 70 nm,不用 UV激发,仅在自然光源下就可看到强烈的绿色荧光,发光强度增加 6倍,若从 3′端截短 12个氨基酸密码,对发光无影响,但截短 75个氨基酸密码,则激发波长降到 360 nm,发光强度减少 12倍。

The subcellular localization of TIAR full length and truncated mutants were observed by using fluorescent microscopy.

酶切鉴定及测序结果显示TIAR全长及缺失体的pEGFP-C3重组表达质粒构建成功。

Subcloning technique was performed to construct the expression plasmids of EGFP-fused TIAR full length and truncated mutants.

结果细胞免疫荧光结果显示在SMCC-7721细胞中TIAR主要浓集于细胞核,细胞浆也有少量弥散分布。

Two kinds of new inversion structural styles including tyrannizing fault and truncated inversion structure are first found in Shiwu fault depression even in Songliao basin on the basis of restudying the forms of inversion structures.

将研究区反转构造划分为四个反转构造带,在对反转构造样式再认识的基础上在十屋乃至松辽地区新发现了欺压式断层和截断型反转构造两种反转构造样式。

Further more, except the detachment-type fold in depression formation, the inversion structural styles can be subdivided into five types: reviving type, forward-expanding type, backward-expanding type, tyrannizing type and truncated type according to the mode of association of the thrust faults and the early normal faults.

进而将反转构造样式除坳陷层滑脱褶皱外,依据反转过程中形成的逆断层与早期伸展断层的组合关系归纳为五种类型:复活型、前展型、后展型、欺压型、截断型。

For most readers, the lines will appear truncated, or will be wrapped unattractively.

对于大多数阅读器来说,这些行将被截断,或将被自动换行。

Truncated ballots with some options unranked were not allowed.

截断票数便陷入困境的一些办法是不允许的。

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推荐网络例句

There had been a moment during the breakfast meeting, though, after the backslapping and the small talk and when all of us were seated, with Vice President Cheney eating his eggs Benedict impassively and Karl Rove at the far end of the table discreetly checking his BlackBerry, that I witnessed a different side of the man.

那次早餐会期间,在表示过关心和寒暄以后,我们所有人坐了下来,我注意观察了一下,副总统切尼面无表情地吃着班尼迪克蛋(Eggs benedict,源自美国,以英式松饼、火腿、水煮蛋以及荷兰酱组合而成),卡尔。罗夫在餐桌的远端谨慎地经常查看他的黑莓手机,我目睹了他所不为人知的一面。

Talk Undelete failed; someone else may have undeleted the page first.

Talk 无法删除选定的页面或图像(它可能已经被其他人删除了)。

When you leave a part of my life.

当你离开了,我只是希望你能幸福在以后的日子里。