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The present invention relates to a process for rapid solution synthesis of a peptide, the process comprising repetitive cycles of steps-: a coupling step, using an excess of an activated carboxylic component to acylate an amino component, a quenching step in which a scavenger is used to remove residual activated carboxylic functions, wherein the scavenger may also be used for deprotection of the growing peptide, one or more aqueous extractions and optionally, a separate deprotection step, followed by one or more aqueous extractions, characterised in that the process comprises at least one step, referred to as step, in which an amine or a thiol comprising a free anion or a latent anion is used as a scavenger of residual activated carboxylic functions.

本发明涉及快速溶液合成肽的方法,该方法包括步骤-的重复循环:偶联步骤,使用过量的活化羧基成分酰化氨基成分,淬灭步骤,在该步骤中使用清除剂除去残余的活化羧基官能团,其中该清除剂还可以用于使生长中的肽去保护,一或多个水抽提和任选地,独立的去保护步骤,之后进行一或多次水抽提,其特征在于该方法包括至少一个步骤,称作步骤,在该步骤中包含自由阴离子或潜阴离子的胺或硫醇用作残余活化羧基官能团的清除剂。

Alternating minimization cost function and maximization poster probability is proposed to restore the blurred image. The method is divided two step, one step is EM step, it is to estimate the PSF by using minimimum-error criteria, the other step is M step, we use MAP criteria to restore the blurred image.

EMM方法分为两个步骤,第一个步骤是EM步骤,利用最小恢复残差准则,估计系统的点扩散函数PSF,第二个步骤是M步骤,利用最大后验概率准则恢复模糊图像。

Based on the systematic analyses of the applications and developments of step motor, the driving technique of step motor are analyzed in this paper, and upon these statements, a mathematical model of VR step motor is built. With this model, the coil current of step motor with chopping driver and the non-loaded starting frequency are simulated for the base of designing of step motor servo system.

本文在系统分析了步进电机应用和发展的基础上,对步进电机的驱动技术作了详尽的论述,建立了三相反应式步进电动机的数学模型,并利用该模型对斩波驱动的步进电机绕组电流及空载运行的启动频率等进行了仿真,为步进电机驱动系统的设计提供了依据。

A variable-step modified Euler time integration method is proposed and analyzed. Four major factors that affect the efficiency of the variable-step modified Euler time integration method are discussed. These include stability and accuracy analysis for the damped fix-step modified Euler integration formula, adjustment method of the fix-step basic integration formula to accommodate step-size changes, suitable range of the step-size selection strategy based on truncation error concept and development of the step-size selection strategy based on apparent frequency concept to satisfied the specified error bound and stability condition.

提出了变步长修正Euler时间积分法,详细讨论了影响变步长修正Euler时间积分算法效率的四个主要因素,它们包括:有阻尼问题的修正Euler法的稳定性和精度分析,调整固定步长的基本积分公式使之容纳步长大小变化的研究,基于截断误差概念的步长大小选择策略适用范围的讨论,同时满足规定误差界和稳定性条件的、基于表观频率概念的步长大小选择策略的建立。

One of the methods for estimating the vanishing point from the image of the invention includes: a dividing step for dividing the image into snippets; a first detecting step for detecting a local direction of each snippet; a combining step for combining lines of directional pencil of lines according to the local direction detected in the first detecting step; and a first calculating step for calculating at least one vanishing point according to the combined lines of directional pencil of lines in the combining step.

根据本发明的用于从图像检测消失点的方法之一包括:用于将图像划分成小片的划分步骤;用于检测每片的局部方向的第一检测步骤;用于根据在第一检测步骤中检测出的局部方向组合方向线束的直线的组合步骤;和用于根据在所述组合步骤中组合的方向线束的直线计算至少一个消失点的第一计算步骤。

The best survuval rate of each stage thawed-embryos existed in groups of Zygote EDT3O-two-step method with I mm equilibration(66%), of Morulae EDT3O-two-step method with I mm equilibration(94%) and EDT4O-two--step method with 0.5 mm equilibration(94%), of Blastocyte EDT3O-two-step method with I mm equilibration(91%) and EDT4O-one-step method with I mm equilibration(91%).

总之,我们的研究表明,DET玻璃液冷冻方法是简单、经济而又高效的,冷冻小鼠各阶段胚胎都能获得较高的存活率,其中受精卵的EDT30二步法1分钟平衡组(66%)、桑椹胚的EDT30二步法1分钟平衡组(94%)和EDT40二步法0.5分钟平衡组(94%)、囊胚的EDT30二步法1分钟平衡组(91%)和EDT40一步法1分钟平衡组(91%)冷冻效果最好。

Through the actual design experience of Xuhui Campus general plan of Shanghai Jiaotong University, it makes the conclusion of exterior space design steps for hisoried campuses. Step 1: research the buildings in campus and analysis in grade. Step 2: make the function distribution area. Step 3: make out the buildings distribution plan. Step 4: design the campus general plot plan. Step 5: finish the exterior space plan of historied campus. Step 6: set up the evaluation standard of historied campus exterior space.

以上海交通大学徐汇校区总体规划实际项目为例,总结出老校园建筑外部空间的设计步骤——第一步:调研校园建筑并分级研究;第二步:确定校园功能分区;第三步:完成校园房产资源配置方案;第四步:完成校园总体规划方案;第五步:完成校园建筑外部空间设计;第六步:建立校园建筑外部空间评价标准。

The results showed high class PbSt2 could be synthesized by this new saponifying process.Compared with metathetical process,the reaction time shortened,liquid-solid ratio and moisture content of filter cake decreased.Powder product could be prepared by one-step process,and particle product by two-step process.For the optimum processing conditions,the reaction temperature and time for one-step process were 65℃ and 10min,the amount of alkali added in the first step was 50%;reaction temperture and time were 65℃ and 10min for the two-step process.

结果表明,用皂化法工艺可得到优等品PbSt2,较复分解工艺,反应时间缩短、液-固比降低、滤饼湿含量比减小,一步式工艺得到粉状产品,二步式工艺得到颗粒状产品;最佳工艺条件为:一步式工艺反应温度65℃,反应时间10min;二步式工艺第一步的加碱量比50%,反应温度65℃,反应时间10min。

The process of three-step countercurrentleaching is presented, in the first leaching step Fe as leading impurity inthe matte is leached priorly, Co and Ni are enriched in the residue of thefirst step and then are leached in the second leaching step with a lowconcentration acid, the residue from the second step containing hardleaching Fe, Co and Ni are leached with a high concentration sulfuricacid in the third step, and Cu stays in the final resude, the leachate of thethird leaching step is diluted and divided into two parts as the leachantsto the first and second leaching step.

在此基础上,提出了三段逆流选择性浸出技术:即第一段用低酸优先浸出钴冰铜中杂质铁,将钴镍富集到第一段的浸出渣中,第二段再用低酸浸出渣中部分钴镍,第三段用高酸浸出第二段渣中难浸的钴镍铁,将铜留在最终的浸出渣中,第三段浸出液经稀释,分流,分别返回至第一段,第二段作低酸浸出介质。

For example, an objective polyester can be produced in a method of producing an ester comprising, a first step wherein a diol is reacted with an ester produced beforehand from a dibasic acid and a monofunctional alcohol, or an ester produced beforehand from a monofunctional alcohol and diol, a second step wherein unreacted ester is separated from the product produced in the first step to obtain another ester, and a third step wherein unreacted ester separated in the second step is recycled to the first step, 0.05-5 millimoles of the catalyst relative to one mole of acid being used in said first step.

例如,可以应用制备酯的方法制备目的聚酯,包括,第一步,使二醇与产生的酯反应,该酯由二元酸和单官能团醇预先制得,或由单官能团醇和二醇预先制得;第二步,将未反应的酯与第一步所制得的产物分离,得到另一种酯;第三步,将第二步中分离出的未反应的酯循环回到第一步中,在第一步中相对于1摩尔酸来说,应用了0.05-5毫摩尔所述的催化剂。

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Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来,英国,在此地早期居住的是凯尔特人,已经被征服了三次。

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